##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547227_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2817053
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313798497933835	31.0	31.0	33.0	30.0	34.0
2	31.708247945636806	31.0	31.0	34.0	30.0	34.0
3	31.77704040357068	31.0	31.0	34.0	30.0	34.0
4	35.511079486257444	37.0	35.0	37.0	33.0	37.0
5	35.39908620817571	37.0	35.0	37.0	33.0	37.0
6	35.43174764550046	37.0	35.0	37.0	33.0	37.0
7	35.97579456261561	37.0	35.0	37.0	35.0	37.0
8	35.97468524731342	37.0	35.0	37.0	35.0	37.0
9	37.676322738691816	39.0	37.0	39.0	35.0	39.0
10	36.973005832691115	39.0	37.0	39.0	32.0	39.0
11	36.778801818780124	39.0	37.0	39.0	32.0	39.0
12	36.567399335404765	39.0	35.0	39.0	32.0	39.0
13	36.53246886018829	39.0	35.0	39.0	32.0	39.0
14	37.65201648673277	40.0	36.0	41.0	32.0	41.0
15	37.74558199650486	40.0	36.0	41.0	32.0	41.0
16	37.744011206036944	40.0	36.0	41.0	32.0	41.0
17	37.665583856604755	40.0	36.0	41.0	32.0	41.0
18	37.48191390080343	39.0	36.0	41.0	32.0	41.0
19	37.323789080290645	39.0	36.0	41.0	32.0	41.0
20	37.04880561352591	39.0	35.0	41.0	32.0	41.0
21	36.95848959888224	38.0	35.0	41.0	32.0	41.0
22	36.89721102158887	38.0	35.0	41.0	32.0	41.0
23	36.78148582934009	38.0	35.0	41.0	31.0	41.0
24	36.71640789150932	38.0	35.0	41.0	31.0	41.0
25	36.550554071932616	38.0	35.0	40.0	31.0	41.0
26	36.459871007041755	38.0	35.0	40.0	31.0	41.0
27	36.337252795740795	38.0	35.0	40.0	30.0	41.0
28	36.36748048403775	38.0	35.0	40.0	31.0	41.0
29	36.26932791111847	38.0	35.0	40.0	30.0	41.0
30	36.173978267359544	38.0	35.0	40.0	30.0	41.0
31	35.939702589905124	38.0	35.0	40.0	30.0	41.0
32	35.437345694241465	38.0	34.0	40.0	26.0	41.0
33	35.09953806335912	38.0	34.0	40.0	24.0	41.0
34	34.741386122305826	38.0	34.0	40.0	21.0	41.0
35	34.412449109051195	38.0	34.0	40.0	19.0	41.0
36	34.2394782774765	38.0	33.0	40.0	18.0	41.0
37	34.195111345082964	38.0	33.0	40.0	18.0	41.0
38	34.14996664954475	38.0	33.0	40.0	18.0	41.0
39	34.0275305434438	38.0	33.0	40.0	18.0	41.0
40	33.94657324516081	38.0	33.0	40.0	17.0	41.0
41	33.92222262058967	38.0	33.0	40.0	17.0	41.0
42	33.70701722686793	38.0	33.0	40.0	15.0	41.0
43	33.74350855308722	38.0	33.0	40.0	15.0	41.0
44	33.478376516167785	37.0	33.0	40.0	15.0	41.0
45	33.39787394841347	37.0	33.0	40.0	15.0	41.0
46	33.37758821009047	37.0	33.0	40.0	15.0	41.0
47	33.28166207735531	37.0	32.0	40.0	15.0	41.0
48	33.22199688823746	37.0	32.0	40.0	15.0	41.0
49	33.19831398273302	37.0	32.0	40.0	14.0	41.0
50	32.66039155102868	36.0	31.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	5.0
13	9.0
14	26.0
15	77.0
16	230.0
17	621.0
18	1562.0
19	3162.0
20	5656.0
21	9072.0
22	13416.0
23	19901.0
24	30193.0
25	47714.0
26	70817.0
27	82426.0
28	78123.0
29	72566.0
30	75114.0
31	85122.0
32	100215.0
33	123394.0
34	178269.0
35	249463.0
36	210005.0
37	259705.0
38	389219.0
39	710962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.244514391458022	22.544694757251637	26.173415977619165	21.037374873671176
2	37.95200161303319	23.394377031600044	26.41061421279614	12.243007142570622
3	25.73206822874827	23.456037213357366	38.035493119937755	12.776401437956617
4	22.62051867678741	25.917829731992974	37.84078609809613	13.620865493123487
5	20.443917810563025	29.661635759071626	36.75706491855141	13.137381511813942
6	17.426615686676822	38.002550892723704	34.28945071320987	10.281382707389602
7	79.51178057352844	3.115525338003935	15.33304485219128	2.03964923627635
8	79.48675442031087	2.596862749831118	14.851548763903272	3.0648340659547406
9	73.43642451881452	5.138028996969528	17.69217689550037	3.7333695887155836
10	32.59250003461064	30.08537645546605	24.376183195701323	12.94594031422199
11	26.1155185933669	24.862649016543177	34.16350349105963	14.8583288990303
12	23.874666184839263	21.399348894039267	37.000368825151675	17.725616095969794
13	20.686831238176918	25.68396831724501	37.58367343461412	16.04552700996396
14	17.886812921162647	28.221052284071334	36.3071266319803	17.58500816278572
15	16.920128943260917	23.769698333684172	42.65450454783776	16.65566817521715
16	18.53759939908834	23.231298807654667	40.418657369953635	17.81244442330336
17	18.69641785227328	23.97242792379128	36.28980356422119	21.041350659714247
18	20.79052115810388	23.27932772297859	37.5340826033447	18.396068515572832
19	22.143388853528847	27.098354202068613	33.21325512867525	17.545001815727286
20	23.589119551531333	24.19748581230101	33.53479682490887	18.678597811258786
21	21.27588653816595	27.59312657589332	33.737739403553995	17.393247482386737
22	20.448284075592472	24.480015107986965	33.54058301352513	21.531117802895437
23	18.355671689528027	27.65833656661767	33.51420793290009	20.471783810954214
24	18.660919762602976	24.515903676643642	37.91827842784641	18.90489813290698
25	18.816188406820885	26.332198932714434	35.15851494451826	19.69309771594642
26	18.363303778807143	27.15145934421539	34.63779346714457	19.8474434098329
27	19.14305481650505	25.704699201612463	34.25469808342264	20.897547898459845
28	18.701813561903165	25.8677064293785	36.52288402099641	18.90759598772192
29	19.363391459088632	23.96550579630557	35.06863378147305	21.602468963132747
30	20.360177817030774	25.240632675352575	34.283025558979546	20.1161639486371
31	22.610721204038406	24.96917878364376	31.74853295269915	20.67156705961869
32	22.43489916590139	25.375880396996436	31.30051156297024	20.88870887413194
33	22.154783740313015	26.064117359524296	30.034791677685867	21.746307222476823
34	19.29828796263329	27.115357787020695	31.677962750434585	21.90839149991143
35	19.95773597443854	27.206197398487003	30.930727962874677	21.905338664199785
36	21.349225591424798	28.038272620358935	30.553667254396704	20.05883453381956
37	20.773695063600154	29.40349365098917	29.9349355514433	19.887875733967377
38	20.058373058653846	29.035555951556468	31.581159459903663	19.32491152988602
39	21.52664504359698	28.90325457135524	29.139316867662767	20.430783517385013
40	22.48541294750223	26.023010571686083	31.022526022762086	20.4690504580496
41	20.49031381376211	25.828410044113475	30.248916154577145	23.43235998754727
42	21.156328972156363	27.398668040679393	28.873684662659876	22.571318324504368
43	21.31227208007801	26.41654239377108	30.045050625600584	22.226134900550328
44	21.397715981914434	28.09496306956241	29.625037228621544	20.882283719901615
45	19.794409263865465	27.837317934735346	29.865678778496534	22.50259402290266
46	21.79483311105613	28.10689042769163	28.938610668666865	21.159665792585372
47	21.145147073910216	26.56829672711163	30.051227293203215	22.23532890577494
48	21.136734026658356	25.01720769896768	30.885148415738005	22.960909858635958
49	21.646060617247883	24.552289218555703	30.42512867170053	23.37652149249588
50	20.02326544796992	25.766785360445827	32.17340958796303	22.03653960362123
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2043.0
1	2389.0
2	2735.0
3	37617.5
4	72500.0
5	51541.0
6	30582.0
7	30630.0
8	30678.0
9	31807.5
10	32937.0
11	32882.0
12	32827.0
13	32381.5
14	31936.0
15	30468.0
16	29000.0
17	27626.5
18	26253.0
19	24706.0
20	23159.0
21	22476.5
22	21794.0
23	22102.5
24	22411.0
25	23760.0
26	25109.0
27	27667.0
28	30225.0
29	34946.0
30	39667.0
31	45458.5
32	51250.0
33	58741.5
34	66233.0
35	75581.0
36	84929.0
37	93244.5
38	101560.0
39	113315.5
40	125071.0
41	143943.5
42	162816.0
43	181066.0
44	199316.0
45	218585.5
46	237855.0
47	252148.5
48	266442.0
49	259264.0
50	252086.0
51	225343.0
52	198600.0
53	174390.5
54	150181.0
55	134302.0
56	118423.0
57	106832.0
58	95241.0
59	87048.0
60	78855.0
61	71073.5
62	63292.0
63	53342.0
64	43392.0
65	34128.5
66	24865.0
67	19728.0
68	14591.0
69	12219.0
70	9847.0
71	8415.5
72	6984.0
73	6139.0
74	5294.0
75	4183.0
76	3072.0
77	2298.0
78	1524.0
79	1136.0
80	748.0
81	599.5
82	451.0
83	331.5
84	212.0
85	123.0
86	34.0
87	27.0
88	20.0
89	12.5
90	5.0
91	4.0
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2817053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.92380070364679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3548520947901	25.138919331252975
2	7.670230844528094	5.050663033524147
3	3.2511019555552414	3.2111589855581135
4	1.9867809419726588	2.6164951910124583
5	1.3758594163350375	2.264926060982529
6	1.0317297377947166	2.0381078560307406
7	0.8403108113318944	1.9366357976987725
8	0.6868710176530335	1.8091523595455627
9	0.5709319557644906	1.691752493424004
>10	5.762504604938506	39.7370987657026
>50	0.41117450518022264	8.720830782478009
>100	0.05395200234077972	2.8241701136504265
>500	0.001958882725649283	0.42444128800336073
>1k	0.0016324022713737811	0.956193200484747
>5k	0.0	0.0
>10k+	1.0882681809158542E-4	1.5794547406515764
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44082	1.564826788846358	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT	3132	0.11118001684739336	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07312606472082706	0.0
2	0.0	0.0	0.0	0.26957249295629154	0.0
3	0.0	0.0	0.0	0.5262591793622626	0.0
4	0.0	0.0	0.0	0.7648063419467082	0.0
5	3.549808967030439E-5	0.0	0.0	1.2562418953424022	0.0
6	3.549808967030439E-5	0.0	0.0	2.324343915432191	0.0
7	3.549808967030439E-5	0.0	0.0	3.0531196963635403	0.0
8	7.099617934060878E-5	0.0	0.0	4.872503286235651	0.0
9	7.099617934060878E-5	0.0	0.0	6.001023054944298	0.0
10	7.099617934060878E-5	0.0	0.0	7.431347582029873	0.0
11	7.099617934060878E-5	0.0	0.0	8.061545167946788	0.0
12	7.099617934060878E-5	0.0	0.0	8.767921654296174	0.0
13	7.099617934060878E-5	0.0	0.0	9.25612688153187	0.0
14	7.099617934060878E-5	0.0	0.0	9.495100021192359	0.0
15	7.099617934060878E-5	0.0	0.0	9.67692123648366	0.0
16	7.099617934060878E-5	0.0	0.0	9.98025241271641	0.0
17	1.0649426901091318E-4	0.0	0.0	10.267715942866534	0.0
18	1.0649426901091318E-4	0.0	0.0	10.807641886751865	0.0
19	1.0649426901091318E-4	0.0	0.0	10.999438065240518	0.0
20	1.0649426901091318E-4	0.0	0.0	11.251758486617042	0.0
21	1.0649426901091318E-4	0.0	0.0	11.460913230954477	0.0
22	1.0649426901091318E-4	0.0	0.0	11.686184108002228	0.0
23	1.0649426901091318E-4	0.0	0.0	11.940101943413914	0.0
24	1.0649426901091318E-4	0.0	0.0	12.151386573131568	0.0
25	1.0649426901091318E-4	0.0	0.0	12.338709992321764	0.0
26	1.0649426901091318E-4	0.0	0.0	12.516129444493945	0.0
27	1.0649426901091318E-4	0.0	0.0	12.741364823452026	0.0
28	1.0649426901091318E-4	0.0	0.0	12.938237228763533	0.0
29	1.0649426901091318E-4	0.0	0.0	13.142741723354158	0.0
30	1.0649426901091318E-4	0.0	0.0	13.436630407734608	0.0
31	1.0649426901091318E-4	0.0	0.0	13.661475307706315	0.0
32	1.0649426901091318E-4	0.0	0.0	13.892567871459997	0.0
33	1.0649426901091318E-4	0.0	0.0	14.127139248001368	0.0
34	1.0649426901091318E-4	0.0	0.0	14.356811888168238	0.0
35	1.0649426901091318E-4	0.0	0.0	14.59958332342345	0.0
36	1.0649426901091318E-4	0.0	0.0	14.831776327956911	0.0
37	1.0649426901091318E-4	0.0	0.0	15.074725253660475	0.0
38	1.0649426901091318E-4	0.0	0.0	15.330417993555677	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	33745	0.0	43.28286	1
TCACGAC	390	0.0	41.74359	25
CGGTCTA	395	0.0	41.21519	31
CTCACGA	400	0.0	40.699997	24
CGACGGT	395	0.0	40.65823	28
GTTTTTT	37685	0.0	39.113705	2
TCGTAAG	80	0.0	38.5	1
ACGGTCT	440	0.0	37.0	30
GACCGAT	1510	0.0	36.569534	9
TAGGGAC	2560	0.0	36.523438	5
GCGCGAC	555	0.0	36.468468	9
TACGGGA	810	0.0	36.12346	4
GTTAGCG	165	0.0	36.0	1
ATAGGGA	3625	0.0	35.988968	4
AGGGCGA	2980	0.0	35.953022	6
ACGGGTA	355	0.0	35.94366	5
TAGGGAT	3540	0.0	35.920906	5
AGGGATT	3750	0.0	35.904	6
ACGGGAT	990	0.0	35.77778	5
CACGACG	450	0.0	35.68889	26
>>END_MODULE