FastQCFastQC Report
Thu 26 May 2016
SRR1547226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547226_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2383390
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160840.6748371017752025No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27330.11466860228498063No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT207.858185E-444.011
TACGCTA254.4442837E-544.01
TCGCTAA254.4442837E-544.027
CGTCAAT254.4442837E-544.033
CGTTTTT103900.042.2848851
CGGTCTA7000.041.48571431
CGACGGT7150.040.92307728
TACGCGG1700.040.117652
CACGACG7350.040.1088426
TCACGAC7550.039.62914325
AGGGTAC8700.039.1954046
TCGATAG900.039.111111
GTCGTAA452.3526809E-839.1111127
TACGGGA5800.038.6896554
CGGTTTA404.127014E-738.538
CGGCATA404.127014E-738.513
ACGGTCT7550.038.46357730
GACGGTC7650.038.24836729
TTTAGCG1500.038.133331
ACGTATC357.293498E-637.71428710
Produced by FastQC (version 0.11.5)