FastQCFastQC Report
Thu 26 May 2016
SRR1547225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547225_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2024455
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230211.137145552753704No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC27010.13341862377775748No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC207.8579027E-444.00000431
CGTTTTT138750.041.859461
TAACGCC2700.037.4814812
ACGGGAC5200.037.2307665
GTTTTTT163700.036.904092
ATGGTCG1850.036.86486444
GACCGAT12250.036.8163269
ATAACGC2750.036.811
AGGGCGA21750.036.717246
TCGTAAG900.036.6666681
CGTAAGG2950.036.5423742
CGCATCG1750.036.4571421
ATACGAA3500.036.4571419
TAGGGCG10350.036.1352655
TATAGCG1100.036.0000041
TAGGGAT18350.035.9673045
CATACGA3550.035.9436618
GGGCGAT44150.035.877697
ACCCGCT12150.035.8518534
TACGGGA6350.035.685044
Produced by FastQC (version 0.11.5)