FastQCFastQC Report
Thu 26 May 2016
SRR1547224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547224_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4166103
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311980.7488533048750835No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC63000.15122045710343696No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45040.10811062520537779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT183550.042.4658131
TCACGAC7050.039.9432625
CGACGGT7050.039.63120728
TCGTAAG1700.038.8235321
TCTCACG7350.038.31292723
CGGTCTA7500.037.8431
AGGGTAC19150.037.566586
TAGGGAC31550.037.2361345
CATATGC28300.036.7703233
CACGACG7450.036.61744726
TTACGGG11300.036.601773
ATAGGGC27200.036.5588264
TAGCATA28350.036.47266430
ACGGTCT7800.036.38461730
GGCGATA19100.036.2827228
TAGGGTA23500.036.136175
ACGGGTA2750.036.05
GGGCGAT88400.035.8619887
TACGGGA9100.035.7802164
AGGGCGA44200.035.7375566
Produced by FastQC (version 0.11.5)