##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547224_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 4166103 Sequences flagged as poor quality 0 Sequence length 50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33292119757961 31.0 31.0 33.0 30.0 34.0 2 31.73195789926461 31.0 31.0 34.0 30.0 34.0 3 31.87724379354039 31.0 31.0 34.0 30.0 34.0 4 35.566047934964644 37.0 35.0 37.0 33.0 37.0 5 35.454035101868584 37.0 35.0 37.0 33.0 37.0 6 35.49055628245389 37.0 35.0 37.0 33.0 37.0 7 35.955893313247415 37.0 35.0 37.0 35.0 37.0 8 35.945170582676425 37.0 35.0 37.0 35.0 37.0 9 37.662088287303504 39.0 37.0 39.0 35.0 39.0 10 37.00538008781828 39.0 37.0 39.0 32.0 39.0 11 36.80193936635748 39.0 37.0 39.0 32.0 39.0 12 36.38245429841749 38.0 35.0 39.0 32.0 39.0 13 36.24338764548068 39.0 35.0 39.0 32.0 39.0 14 37.31127290899913 39.0 35.0 41.0 32.0 41.0 15 37.46196049401563 39.0 35.0 41.0 32.0 41.0 16 37.49442896635057 39.0 35.0 41.0 32.0 41.0 17 37.42214510779018 39.0 35.0 41.0 32.0 41.0 18 37.36382561832965 39.0 35.0 41.0 32.0 41.0 19 37.325321049431565 39.0 35.0 41.0 32.0 41.0 20 37.172586947562266 39.0 35.0 41.0 32.0 41.0 21 37.066032452870225 39.0 35.0 41.0 32.0 41.0 22 37.00963610357209 39.0 35.0 41.0 32.0 41.0 23 36.92042731540723 39.0 35.0 41.0 31.0 41.0 24 36.85956396181275 38.0 35.0 41.0 31.0 41.0 25 36.65798421210421 38.0 35.0 41.0 31.0 41.0 26 36.54835514148354 38.0 35.0 41.0 31.0 41.0 27 36.46907385631128 38.0 35.0 40.0 31.0 41.0 28 36.515935875805276 38.0 35.0 40.0 31.0 41.0 29 36.4142893250599 38.0 35.0 40.0 30.0 41.0 30 36.35974218592291 38.0 35.0 40.0 30.0 41.0 31 36.237108396023814 38.0 35.0 40.0 30.0 41.0 32 36.01624131712538 38.0 35.0 40.0 30.0 41.0 33 35.907058226836924 38.0 35.0 40.0 29.0 41.0 34 35.78019074420388 38.0 35.0 40.0 29.0 41.0 35 35.56185528778333 38.0 35.0 40.0 27.0 41.0 36 35.44551394912704 38.0 34.0 40.0 27.0 41.0 37 35.396655339534334 38.0 34.0 40.0 27.0 41.0 38 35.35501618658972 38.0 34.0 40.0 27.0 41.0 39 35.27480861610959 38.0 34.0 40.0 27.0 41.0 40 35.16334209691887 38.0 34.0 40.0 26.0 41.0 41 35.161384632113034 37.0 34.0 40.0 26.0 41.0 42 34.95874201862028 37.0 34.0 40.0 26.0 41.0 43 34.97594778621652 37.0 34.0 40.0 26.0 41.0 44 34.72422549322472 37.0 34.0 40.0 25.0 41.0 45 34.60577810966267 37.0 34.0 40.0 24.0 41.0 46 34.603464916733934 36.0 34.0 40.0 25.0 41.0 47 34.48907720236394 36.0 33.0 40.0 24.0 41.0 48 34.44183233107775 36.0 33.0 40.0 24.0 41.0 49 34.42216623064768 36.0 34.0 40.0 25.0 41.0 50 33.89901737907104 35.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 2.0 11 8.0 12 7.0 13 21.0 14 38.0 15 119.0 16 280.0 17 722.0 18 1575.0 19 3162.0 20 5759.0 21 9307.0 22 14231.0 23 20699.0 24 29073.0 25 42337.0 26 59055.0 27 73241.0 28 81355.0 29 90289.0 30 104081.0 31 125669.0 32 155425.0 33 197989.0 34 311681.0 35 462442.0 36 335638.0 37 425345.0 38 613740.0 39 1002809.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.87717490422104 23.298655842162326 27.456570324833546 15.367598928783085 2 32.931302946662626 24.949119116834126 28.654164335351282 13.465413601151962 3 30.028086199501068 24.97245027307294 30.979118855198735 14.020344672227258 4 25.597662851830595 27.42450198662875 31.5302334099757 15.447601751564951 5 22.325660215313928 31.746478663633614 30.693672239980625 15.23418888107183 6 19.18061075302267 41.34756149811947 28.23427553279408 11.23755221606379 7 85.96249300605386 3.8734760038337988 7.919367332012675 2.2446636580996677 8 85.9174389111359 3.11518942282512 7.542372332129091 3.4249993339098914 9 80.73076925846529 4.681545319450815 10.647408381405835 3.9402770406780627 10 41.11516205912336 25.16975696472219 17.07677414600647 16.638306830147982 11 34.394972951940936 24.517684752393304 24.167309353609355 16.9200329420564 12 31.2571004605503 20.714778295207775 28.35517988873535 19.672941355506573 13 24.27491591062439 25.929963805503608 30.23938678424417 19.55573349962783 14 20.006514481279027 29.818729877777866 30.23014553408785 19.944610106855258 15 18.69384890387972 22.497451455232863 38.79087963019637 20.017820010691047 16 21.17228018606357 20.122234135833896 36.90456044893754 21.800925229165003 17 21.28528747368944 21.178160981617594 30.051897420683073 27.484654124009893 18 23.942206901749667 22.58340708331023 31.32949425398268 22.14489176095742 19 27.056508204429896 26.106795727325995 25.88586984047202 20.950826227772094 20 28.387392246423097 24.41012620187259 25.066855044150373 22.135626507553944 21 25.356958289317376 27.27373759122134 26.788751982368176 20.580552137093104 22 23.73405074238443 23.542624846289208 26.718710507157407 26.004613904168956 23 22.374674846013168 27.688441692392146 25.672841021933447 24.26404243966124 24 23.31857853730453 24.420279575420963 30.584961533596267 21.676180353678244 25 23.45702926691923 24.363199853676203 28.750873418156008 23.428897461248557 26 20.64797725836351 27.44502476294993 27.55063905045074 24.35635892823581 27 22.276477561884573 24.96294498719787 28.456953656690676 24.303623794226883 28 21.224391235646358 26.054036590069906 31.324981643516736 21.396590530767 29 22.380723664297307 25.348221107351403 29.405994042874116 22.86506118547717 30 23.080394315743032 25.51336344780722 29.08675085565575 22.319491380793995 31 27.001036700244807 24.51691664848421 24.518932921245586 23.9631137300254 32 26.566265884448846 26.84460753850781 24.485520401199874 22.103606175843467 33 25.069879453292444 25.660575362635058 24.45947687803206 24.810068306040442 34 21.21006129709227 27.73935257961697 27.67415976033238 23.37642636295838 35 21.50599733131898 26.322488906299245 27.34581934244065 24.82569441994113 36 25.263825690339388 26.704356565356164 25.186655250722318 22.845162493582134 37 22.18471314799466 28.069013176102466 25.266802092987138 24.47947158291574 38 22.613627171483756 28.289627020743367 24.348221827448818 24.74852398032406 39 22.122040669661793 25.760308854581847 24.148490807836485 27.969159667919875 40 23.330436141401208 23.519677742004937 27.303717646923275 25.846168469670577 41 20.689262843477465 23.165389813934027 27.62728141863031 28.51806592395819 42 23.118559478726283 26.312551561975305 24.26865106311582 26.300237896182594 43 22.32470008542756 24.92185142806119 26.712781705109066 26.040666781402187 44 23.128760858768974 26.195463722332356 27.342962956028693 23.332812462869974 45 21.91496465641872 26.832149853232146 26.843263356666892 24.409622133682245 46 24.106245092836158 25.913281548727912 27.02220756423929 22.958265794196638 47 23.020458207586323 25.520276382989092 27.152569199561317 24.306696209863272 48 23.09438820883689 24.500018362484077 27.44768912338461 24.95790430529442 49 23.17863960636595 23.263635104556947 27.9205770956695 25.637148193407604 50 21.749438264008354 24.69552481059638 28.658868971794504 24.896167953600763 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3967.0 1 4392.5 2 4818.0 3 28238.0 4 51658.0 5 35364.0 6 19070.0 7 19304.5 8 19539.0 9 20080.5 10 20622.0 11 20526.0 12 20430.0 13 20157.5 14 19885.0 15 18428.0 16 16971.0 17 15858.5 18 14746.0 19 14112.0 20 13478.0 21 13761.5 22 14045.0 23 14916.0 24 15787.0 25 17458.0 26 19129.0 27 21923.5 28 24718.0 29 31153.0 30 37588.0 31 42104.5 32 46621.0 33 54047.0 34 61473.0 35 68116.0 36 74759.0 37 87323.5 38 99888.0 39 119284.5 40 138681.0 41 173579.5 42 208478.0 43 235758.5 44 263039.0 45 292508.0 46 321977.0 47 348078.5 48 374180.0 49 384207.5 50 394235.0 51 356049.0 52 317863.0 53 284544.5 54 251226.0 55 237236.0 56 223246.0 57 221032.0 58 218818.0 59 213782.0 60 208746.0 61 195575.5 62 182405.0 63 167190.0 64 151975.0 65 125726.5 66 99478.0 67 83871.0 68 68264.0 69 61532.0 70 54800.0 71 46488.0 72 38176.0 73 30862.5 74 23549.0 75 19053.0 76 14557.0 77 10813.0 78 7069.0 79 5115.0 80 3161.0 81 2386.5 82 1612.0 83 1187.5 84 763.0 85 516.5 86 270.0 87 248.5 88 227.0 89 153.5 90 80.0 91 44.5 92 9.0 93 13.5 94 18.0 95 12.5 96 7.0 97 4.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4166103.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.676045863155505 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7388704645786 17.06747367403266 2 7.606687079990718 3.297657960251024 3 2.687787900681203 1.7478184141680064 4 1.3989996109521312 1.212991189181404 5 0.8792021760900588 0.9528813345957119 6 0.6732338001889737 0.8755828037713598 7 0.507338465416594 0.7697964291159117 8 0.4265090347090261 0.7396023517922427 9 0.34111017915396996 0.6654527898957581 >10 4.383239601755102 22.916944402931946 >50 1.6274658734816552 25.213581485318358 >100 0.7201613868288236 21.514991486541117 >500 0.0061511123752354974 0.9176378902870227 >1k 0.00301963698420396 1.1985558590772654 >5k 1.1183840682236889E-4 0.1527255094392664 >10k+ 1.1183840682236889E-4 0.7563064196009895 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31198 0.7488533048750835 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC 6300 0.15122045710343696 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 4504 0.10811062520537779 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.07570624154035557 0.0 2 0.0 0.0 0.0 0.25213010816103204 0.0 3 0.0 0.0 0.0 0.4389713840488341 0.0 4 0.0 0.0 0.0 0.6313574100304289 0.0 5 0.0 0.0 0.0 1.0201140010220582 0.0 6 0.0 0.0 0.0 1.9109945193385762 0.0 7 0.0 0.0 0.0 2.4597567558939373 0.0 8 0.0 0.0 0.0 3.9844430154511303 0.0 9 0.0 0.0 0.0 4.876787731844364 0.0 10 0.0 0.0 0.0 6.11139474948171 0.0 11 0.0 0.0 0.0 6.749833117424125 0.0 12 0.0 0.0 0.0 7.366524543440237 0.0 13 2.4003247159275705E-5 0.0 0.0 7.718268127312263 0.0 14 2.4003247159275705E-5 0.0 0.0 7.898532513478424 0.0 15 2.4003247159275705E-5 0.0 0.0 8.034270876164127 0.0 16 2.4003247159275705E-5 0.0 0.0 8.263117834580662 0.0 17 2.4003247159275705E-5 0.0 0.0 8.492084809232994 0.0 18 2.4003247159275705E-5 0.0 0.0 8.908157095491879 0.0 19 2.4003247159275705E-5 0.0 0.0 9.066146468294232 0.0 20 4.800649431855141E-5 2.4003247159275705E-5 0.0 9.254787987718979 0.0 21 4.800649431855141E-5 2.4003247159275705E-5 0.0 9.438508841476075 0.0 22 4.800649431855141E-5 2.4003247159275705E-5 0.0 9.627462403113894 0.0 23 4.800649431855141E-5 2.4003247159275705E-5 0.0 9.845027835365569 0.0 24 4.800649431855141E-5 2.4003247159275705E-5 0.0 10.020875624054423 0.0 25 4.800649431855141E-5 2.4003247159275705E-5 0.0 10.17003180190216 0.0 26 4.800649431855141E-5 2.4003247159275705E-5 0.0 10.324132648664712 0.0 27 4.800649431855141E-5 2.4003247159275705E-5 0.0 10.518870992867916 0.0 28 4.800649431855141E-5 2.4003247159275705E-5 0.0 10.677196411130497 0.0 29 4.800649431855141E-5 2.4003247159275705E-5 0.0 10.83984241388175 0.0 30 4.800649431855141E-5 2.4003247159275705E-5 0.0 11.064776843011323 0.0 31 4.800649431855141E-5 2.4003247159275705E-5 0.0 11.229823170478502 0.0 32 4.800649431855141E-5 2.4003247159275705E-5 0.0 11.418392680161773 0.0 33 4.800649431855141E-5 2.4003247159275705E-5 0.0 11.598345024114863 0.0 34 4.800649431855141E-5 2.4003247159275705E-5 0.0 11.766271741241155 0.0 35 1.4401948295565423E-4 2.4003247159275705E-5 0.0 11.958897799694343 0.0 36 1.4401948295565423E-4 2.4003247159275705E-5 0.0 12.124928260295052 0.0 37 1.4401948295565423E-4 2.4003247159275705E-5 0.0 12.308529097816352 0.0 38 1.4401948295565423E-4 2.4003247159275705E-5 0.0 12.51442895194862 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 18355 0.0 42.465813 1 TCACGAC 705 0.0 39.94326 25 CGACGGT 705 0.0 39.631207 28 TCGTAAG 170 0.0 38.823532 1 TCTCACG 735 0.0 38.312927 23 CGGTCTA 750 0.0 37.84 31 AGGGTAC 1915 0.0 37.56658 6 TAGGGAC 3155 0.0 37.236134 5 CATATGC 2830 0.0 36.77032 33 CACGACG 745 0.0 36.617447 26 TTACGGG 1130 0.0 36.60177 3 ATAGGGC 2720 0.0 36.558826 4 TAGCATA 2835 0.0 36.472664 30 ACGGTCT 780 0.0 36.384617 30 GGCGATA 1910 0.0 36.282722 8 TAGGGTA 2350 0.0 36.13617 5 ACGGGTA 275 0.0 36.0 5 GGGCGAT 8840 0.0 35.861988 7 TACGGGA 910 0.0 35.780216 4 AGGGCGA 4420 0.0 35.737556 6 >>END_MODULE