FastQCFastQC Report
Thu 26 May 2016
SRR1547223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547223_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1350878
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115250.8531488409760171No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15320.11340772445772304No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG207.8569737E-444.01
ATTCACG254.4432567E-544.01
ATATACG302.5280478E-644.01
TATCGAT207.8569737E-444.013
CGTTTTT77400.042.6640851
AGGCGAT1400.042.4285746
TCACGAC2650.041.50943825
CGGTCTA2550.041.41176631
TTCACGG800.041.252
TTTGACG700.040.8571431
ACGGGTA2050.040.7804875
GTAGCAT5450.040.36697429
ATAACGC557.8216544E-1140.011
CACGACG2650.039.8490626
CGCATGG1000.039.62
GTATGCG452.3514076E-839.111111
GCGATTC850.038.8235329
GTTGATC5850.038.73504616
GGTAATC1250.038.728
TACGTAG404.1252497E-738.51
Produced by FastQC (version 0.11.5)