##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547223_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1350878
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.322738248753772	31.0	31.0	33.0	30.0	34.0
2	31.708089849712557	31.0	31.0	34.0	30.0	34.0
3	31.82609680518892	31.0	31.0	34.0	30.0	34.0
4	35.52385189484173	37.0	35.0	37.0	33.0	37.0
5	35.405457783752496	37.0	35.0	37.0	33.0	37.0
6	35.44107832091425	37.0	35.0	37.0	33.0	37.0
7	35.94711661600826	37.0	35.0	37.0	35.0	37.0
8	35.94123303510754	37.0	35.0	37.0	35.0	37.0
9	37.65222322074976	39.0	37.0	39.0	35.0	39.0
10	36.97537527445113	39.0	37.0	39.0	32.0	39.0
11	36.749753863783404	39.0	35.0	39.0	32.0	39.0
12	36.21707215603482	38.0	35.0	39.0	32.0	39.0
13	35.98988065539597	38.0	35.0	39.0	31.0	39.0
14	37.02911661896929	39.0	35.0	41.0	31.0	41.0
15	37.25506226320956	39.0	35.0	41.0	32.0	41.0
16	37.31971132848414	39.0	35.0	41.0	32.0	41.0
17	37.25713054768824	39.0	35.0	41.0	32.0	41.0
18	37.191454002507996	39.0	35.0	41.0	32.0	41.0
19	37.13106809053075	38.0	35.0	41.0	32.0	41.0
20	36.930277197496736	38.0	35.0	41.0	32.0	41.0
21	36.8010442097658	38.0	35.0	41.0	31.0	41.0
22	36.72227395812205	38.0	35.0	41.0	31.0	41.0
23	36.6283491181291	38.0	35.0	40.0	31.0	41.0
24	36.545864245327856	38.0	35.0	40.0	31.0	41.0
25	36.38155259024131	38.0	35.0	40.0	31.0	41.0
26	36.24403388018755	38.0	35.0	40.0	31.0	41.0
27	36.15347129792624	38.0	35.0	40.0	30.0	41.0
28	36.22313413942636	38.0	35.0	40.0	31.0	41.0
29	36.154300388340026	38.0	35.0	40.0	30.0	41.0
30	36.141996538547524	38.0	35.0	40.0	30.0	41.0
31	35.99145888821936	38.0	35.0	40.0	30.0	41.0
32	35.72730994212653	38.0	35.0	40.0	29.0	41.0
33	35.61098485577528	38.0	35.0	40.0	29.0	41.0
34	35.47631392324103	38.0	35.0	40.0	28.0	41.0
35	35.25122327848999	38.0	34.0	40.0	27.0	41.0
36	35.06818602420056	38.0	34.0	40.0	25.0	41.0
37	35.0117819669874	37.0	34.0	40.0	25.0	41.0
38	34.97217883480226	37.0	34.0	40.0	26.0	41.0
39	34.892176791686595	37.0	34.0	40.0	25.0	41.0
40	34.770926019966275	37.0	34.0	40.0	24.0	41.0
41	34.77516474470678	37.0	34.0	40.0	25.0	41.0
42	34.57299548885984	37.0	34.0	40.0	24.0	41.0
43	34.5936820349432	37.0	34.0	40.0	24.0	41.0
44	34.33196336012578	36.0	34.0	40.0	23.0	41.0
45	34.23494497652638	36.0	33.0	40.0	23.0	41.0
46	34.21982147906769	36.0	33.0	40.0	23.0	41.0
47	34.10287013335031	36.0	33.0	40.0	23.0	41.0
48	34.06871827063584	35.0	33.0	40.0	23.0	41.0
49	34.06368154637207	36.0	33.0	40.0	24.0	41.0
50	33.516185769551356	35.0	33.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	2.0
13	4.0
14	12.0
15	18.0
16	103.0
17	241.0
18	587.0
19	1199.0
20	2185.0
21	3479.0
22	5293.0
23	7641.0
24	10821.0
25	16214.0
26	22313.0
27	26208.0
28	28619.0
29	29977.0
30	34676.0
31	41533.0
32	51867.0
33	66822.0
34	108515.0
35	174117.0
36	104267.0
37	127763.0
38	181718.0
39	304680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.647803872740546	23.927623367913313	27.742993815873824	15.681578943472319
2	33.23771650733819	25.075395409504043	28.330833724436992	13.356054358720773
3	28.235192223131918	25.447301680832762	33.15140227318825	13.166103822847067
4	25.02920322930716	26.89206575279189	32.841529731034186	15.237201286866764
5	22.21111010764851	31.559696730570785	31.70530573449268	14.52388742728803
6	19.393979323077286	40.67347310415892	28.805932141910667	11.12661543085312
7	84.39081841587472	3.9921443683293383	9.319568458439623	2.297468757356327
8	84.21959644024109	3.3090330881101035	8.822706417603959	3.6486640540448505
9	78.96864113561698	4.8965931786586205	12.01588892557285	4.1188767601515455
10	42.55173302104261	25.653167791614045	17.495954482936284	14.29914470440706
11	36.742104024197594	23.115706969837394	24.709929394068155	15.432259611896857
12	33.527972178094544	19.82636477905481	28.65203223385087	17.99363080899978
13	22.073866033794317	31.465091592282945	30.022400246358295	16.438642127564442
14	17.109094973787418	34.639175410362746	29.63524463349022	18.616484982359623
15	15.531010202253645	22.79317599368707	43.84363354795918	17.83218025610011
16	17.115017048171634	19.32084170443223	41.95071649697456	21.61342475042158
17	17.363744172308675	21.72305715245936	30.92936593830087	29.98383273693109
18	21.452492379030527	23.085208286758686	33.73183958877116	21.73045974543963
19	26.160837618200905	27.25871618310462	26.27742845764014	20.303017741054337
20	27.92902097746799	24.012309031607593	26.1358908798574	21.922779111067026
21	22.803539623859447	28.527224516203535	27.024498141208902	21.644737718728116
22	22.130791973812585	25.00751363187497	25.720309309945087	27.141385084367354
23	20.048294516603278	29.870647090262775	25.61956001948362	24.461498373650322
24	20.299834626072823	24.624355419216243	34.62725723566451	20.44855271904643
25	20.279625547236684	25.93579879160072	31.03144769549878	22.753127965663815
26	18.56888630949649	29.723927697393844	28.72183868565481	22.985347307454855
27	20.761460324322403	27.164333122606187	28.640188085082443	23.434018467988967
28	18.65475638806761	26.57656723997282	34.66604682288112	20.10262954907845
29	18.72833816229149	24.06731029745099	33.25348403038617	23.950867509871358
30	20.857768058995703	26.35685828031843	31.193860585485883	21.59151307519998
31	26.373884244173052	25.43952895820348	25.01624869159169	23.17033810603178
32	26.381730992732134	25.661532721681752	26.147883080485435	21.808853205100682
33	23.857076656811348	25.86843519548027	25.836826123454525	24.437662024253857
34	19.81962841944276	26.74408791911631	28.76921528072853	24.667068380712394
35	19.991812732163822	25.92950658756749	29.252530576410308	24.82615010385838
36	26.741571037503014	26.71166456186273	25.196723908450654	21.350040492183602
37	21.163421123151018	29.106699494699	27.131095480124777	22.5987839020252
38	21.121596472812495	30.605502495414093	25.655980776946546	22.616920254826862
39	20.349209921251216	28.52700243841413	25.278078405303813	25.845709235030846
40	23.000448597134604	25.52562111456401	27.142865602963408	24.33106468533798
41	18.821536807913077	24.04258563689689	27.149305858856238	29.98657169633379
42	21.963197268739293	26.75104635651776	24.928601990705303	26.35715438403764
43	21.445237837909865	26.07711429159406	26.660586670298876	25.817061200197205
44	21.705513007096126	28.380505123334604	27.976175494752304	21.937806374816972
45	19.222091114075436	29.468020058065942	26.467156915724438	24.842731912134184
46	23.206980941284115	28.22208963355684	26.705298331899698	21.865631093259346
47	22.492112537179523	25.54664447862797	27.93020539234483	24.031037591847674
48	22.535269654254492	23.50404699758231	28.521746597398135	25.438936750765055
49	21.18607305767064	23.1599004499296	29.37311881605889	26.28090767634087
50	20.08353085918936	26.795165810680167	29.352983763152558	23.768319566977922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1612.0
1	1661.0
2	1710.0
3	9804.5
4	17899.0
5	12574.0
6	7249.0
7	7413.5
8	7578.0
9	8015.0
10	8452.0
11	8450.5
12	8449.0
13	8305.0
14	8161.0
15	7749.5
16	7338.0
17	7000.0
18	6662.0
19	6546.0
20	6430.0
21	6313.0
22	6196.0
23	6166.0
24	6136.0
25	6853.0
26	7570.0
27	8442.5
28	9315.0
29	11022.0
30	12729.0
31	15451.0
32	18173.0
33	20514.5
34	22856.0
35	26198.0
36	29540.0
37	32411.5
38	35283.0
39	43665.0
40	52047.0
41	66273.0
42	80499.0
43	94273.0
44	108047.0
45	114093.0
46	120139.0
47	126523.0
48	132907.0
49	129799.0
50	126691.0
51	112883.5
52	99076.0
53	88774.5
54	78473.0
55	69788.0
56	61103.0
57	58070.5
58	55038.0
59	54216.0
60	53394.0
61	48321.5
62	43249.0
63	38235.5
64	33222.0
65	28483.5
66	23745.0
67	20842.5
68	17940.0
69	15902.5
70	13865.0
71	11494.0
72	9123.0
73	7290.0
74	5457.0
75	4593.5
76	3730.0
77	2857.0
78	1984.0
79	1461.0
80	938.0
81	736.0
82	534.0
83	384.5
84	235.0
85	152.5
86	70.0
87	45.0
88	20.0
89	13.0
90	6.0
91	5.0
92	4.0
93	2.0
94	0.0
95	1.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1350878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.363613276074656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42052238475567	23.027098922137213
2	7.423397116903624	4.359555242709712
3	2.841713618198401	2.5032893917839814
4	1.5841082782889844	1.8606057148442476
5	1.0842780251938557	1.591916030776916
6	0.7954903009730311	1.4015081737584196
7	0.6304820160085491	1.2959261066916463
8	0.4912161903964112	1.15391057997975
9	0.4256660854778464	1.1249184886840817
>10	5.195308993037128	36.27455067868773
>50	1.0127486607842382	19.640652646778868
>100	0.09128577044718925	4.048635583732134
>500	0.0027738769923781707	0.5756367933159648
>1k	7.565119070122283E-4	0.28841076794001586
>5k	0.0	0.0
>10k+	2.521706356707428E-4	0.8533848781793794
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11525	0.8531488409760171	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1532	0.11340772445772304	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07254541120663746	0.0
2	0.0	0.0	0.0	0.27041672156923124	0.0
3	0.0	0.0	0.0	0.4957516518886235	0.0
4	0.0	0.0	0.0	0.7319683938890115	0.0
5	0.0	0.0	0.0	1.2254992678835543	0.0
6	0.0	0.0	0.0	2.2836259084832236	0.0
7	0.0	0.0	0.0	2.943863176393427	0.0
8	0.0	0.0	0.0	4.933236013910952	0.0
9	0.0	0.0	0.0	6.171615793580175	0.0
10	0.0	0.0	0.0	7.7921914488206925	0.0
11	0.0	0.0	0.0	8.55480657764802	0.0
12	0.0	0.0	0.0	9.348734674781882	0.0
13	0.0	0.0	0.0	9.790151294195331	0.0
14	0.0	0.0	0.0	9.983803126559172	0.0
15	0.0	0.0	0.0	10.141774460758114	0.0
16	0.0	0.0	0.0	10.427070394217687	0.0
17	0.0	0.0	0.0	10.71295853511568	0.0
18	0.0	0.0	0.0	11.259047819270133	0.0
19	0.0	0.0	0.0	11.453736014651213	0.0
20	0.0	0.0	0.0	11.70423976110352	0.0
21	0.0	0.0	0.0	11.951708444433915	0.0
22	0.0	0.0	0.0	12.19525375348477	0.0
23	0.0	0.0	0.0	12.491653576414746	0.0
24	0.0	0.0	0.0	12.722762529258748	0.0
25	0.0	0.0	0.0	12.91537799860535	0.0
26	0.0	0.0	0.0	13.094002567219245	0.0
27	7.402592980269129E-5	0.0	0.0	13.306975167261589	0.0
28	7.402592980269129E-5	0.0	0.0	13.518763352427088	0.0
29	7.402592980269129E-5	0.0	0.0	13.735141145240355	0.0
30	7.402592980269129E-5	0.0	0.0	14.030134475504079	0.0
31	7.402592980269129E-5	0.0	0.0	14.249251227720046	0.0
32	7.402592980269129E-5	0.0	0.0	14.471032913408909	0.0
33	7.402592980269129E-5	0.0	0.0	14.676899024190194	0.0
34	7.402592980269129E-5	0.0	0.0	14.884393705427136	0.0
35	7.402592980269129E-5	0.0	0.0	15.126754599601147	0.0
36	7.402592980269129E-5	0.0	0.0	15.344538885080667	0.0
37	7.402592980269129E-5	0.0	0.0	15.563803689156238	0.0
38	7.402592980269129E-5	0.0	0.0	15.793876278983003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	20	7.8569737E-4	44.0	1
ATTCACG	25	4.4432567E-5	44.0	1
ATATACG	30	2.5280478E-6	44.0	1
TATCGAT	20	7.8569737E-4	44.0	13
CGTTTTT	7740	0.0	42.664085	1
AGGCGAT	140	0.0	42.428574	6
TCACGAC	265	0.0	41.509438	25
CGGTCTA	255	0.0	41.411766	31
TTCACGG	80	0.0	41.25	2
TTTGACG	70	0.0	40.857143	1
ACGGGTA	205	0.0	40.780487	5
GTAGCAT	545	0.0	40.366974	29
ATAACGC	55	7.8216544E-11	40.0	11
CACGACG	265	0.0	39.84906	26
CGCATGG	100	0.0	39.6	2
GTATGCG	45	2.3514076E-8	39.11111	1
GCGATTC	85	0.0	38.823532	9
GTTGATC	585	0.0	38.735046	16
GGTAATC	125	0.0	38.72	8
TACGTAG	40	4.1252497E-7	38.5	1
>>END_MODULE