FastQCFastQC Report
Thu 26 May 2016
SRR1547222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547222_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences736485
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46600.6327352220343931No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14660.1990536127687597No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9930.13482962993136316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGT302.5264726E-644.00000412
ATTAGCG302.5264726E-644.0000041
TACTGGC302.5264726E-644.0000043
ACGTTAG254.441277E-544.01
GACGTAG207.854638E-444.01
AAGCGGT207.854638E-444.03
ACCCTTA207.854638E-444.031
ACGGGTA750.044.05
TCTAGCG408.305506E-944.01
TGCGCAA254.441277E-544.01
TGCGAAC207.854638E-444.037
TCATCGG207.854638E-444.02
GTCCATA254.441277E-544.033
TTACCTA207.854638E-444.040
CGCATCG351.4451325E-743.99999621
GCTTGCG351.4451325E-743.9999961
GTCTCAC2800.043.21428322
TCACGAC2750.043.225
CGGTCTA2650.043.1698131
ACGGTCT2650.043.1698130
Produced by FastQC (version 0.11.5)