##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547222_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	736485
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.354611431325825	31.0	31.0	33.0	30.0	34.0
2	31.749139493676044	31.0	31.0	34.0	30.0	34.0
3	31.871671520804906	31.0	31.0	34.0	30.0	34.0
4	35.558319585599165	37.0	35.0	37.0	33.0	37.0
5	35.427236128366495	37.0	35.0	37.0	33.0	37.0
6	35.46677800634093	37.0	35.0	37.0	33.0	37.0
7	35.95918043137335	37.0	35.0	37.0	35.0	37.0
8	35.97205238395894	37.0	35.0	37.0	35.0	37.0
9	37.69528232075331	39.0	37.0	39.0	35.0	39.0
10	37.016626272089724	39.0	37.0	39.0	33.0	39.0
11	36.73940677678432	39.0	35.0	39.0	32.0	39.0
12	36.0115766105216	37.0	35.0	39.0	32.0	39.0
13	35.652555041854214	37.0	35.0	39.0	31.0	39.0
14	36.6759472358568	38.0	35.0	41.0	31.0	41.0
15	36.98097449370999	38.0	35.0	41.0	32.0	41.0
16	37.12618043816235	38.0	35.0	41.0	32.0	41.0
17	37.06289876915348	38.0	35.0	41.0	32.0	41.0
18	37.010096607534436	38.0	35.0	41.0	32.0	41.0
19	36.943859005953954	38.0	35.0	41.0	32.0	41.0
20	36.760945572550696	38.0	35.0	41.0	32.0	41.0
21	36.62543025316198	38.0	35.0	40.0	31.0	41.0
22	36.5340937018405	38.0	35.0	40.0	31.0	41.0
23	36.47650121862631	38.0	35.0	40.0	31.0	41.0
24	36.38664738589381	38.0	35.0	40.0	31.0	41.0
25	36.21938532353001	37.0	35.0	40.0	31.0	41.0
26	36.09742493058243	37.0	35.0	40.0	31.0	41.0
27	35.99416009830478	37.0	35.0	40.0	30.0	41.0
28	36.07873751671792	37.0	35.0	40.0	31.0	41.0
29	36.047493160077934	37.0	35.0	40.0	31.0	41.0
30	36.04187593773125	37.0	35.0	40.0	31.0	41.0
31	35.9059682138808	37.0	35.0	40.0	30.0	41.0
32	35.6627602734611	37.0	34.0	40.0	30.0	41.0
33	35.58055221762833	37.0	35.0	40.0	29.0	41.0
34	35.47559963882496	37.0	35.0	40.0	29.0	41.0
35	35.3058012043694	37.0	34.0	40.0	28.0	41.0
36	35.09667678228342	37.0	34.0	40.0	27.0	41.0
37	35.00302789601961	37.0	34.0	40.0	27.0	41.0
38	34.987357515767464	37.0	34.0	40.0	27.0	41.0
39	34.8684508170567	36.0	34.0	40.0	26.0	41.0
40	34.73859481184274	36.0	34.0	40.0	25.0	41.0
41	34.72639089730273	36.0	34.0	40.0	26.0	41.0
42	34.55114225001188	36.0	34.0	40.0	24.0	41.0
43	34.55518985451163	36.0	34.0	40.0	26.0	41.0
44	34.30660502250555	35.0	34.0	40.0	24.0	41.0
45	34.216035628695764	35.0	33.0	40.0	24.0	41.0
46	34.22052994969348	35.0	34.0	40.0	24.0	41.0
47	34.100099798366564	35.0	33.0	40.0	23.0	41.0
48	34.091841653258385	35.0	33.0	40.0	24.0	41.0
49	34.074438719050626	35.0	33.0	40.0	24.0	41.0
50	33.52892862719539	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	0.0
15	15.0
16	54.0
17	133.0
18	371.0
19	631.0
20	1114.0
21	1806.0
22	2725.0
23	3981.0
24	5915.0
25	8243.0
26	11208.0
27	13476.0
28	14648.0
29	16249.0
30	19022.0
31	23321.0
32	29302.0
33	37719.0
34	65190.0
35	112928.0
36	54134.0
37	64762.0
38	92756.0
39	156777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.876121034372726	24.599550567900227	29.374664792901417	15.149663604825625
2	31.4846874002865	25.968485440979787	29.466723694304704	13.08010346442901
3	27.955083946040993	25.783281397448693	32.533588599903595	13.728046056606718
4	24.959639368079458	28.1793926556549	32.24274764591268	14.61822033035296
5	22.567873072771338	31.633502379546087	31.338044902475946	14.460579645206623
6	18.91443817593026	40.94801659232707	28.473492331819383	11.664052899923284
7	85.69651791957746	4.541436689138272	7.790246916094692	1.9717984751895832
8	86.66449418521762	3.0603474612517565	7.0210527030421535	3.254105650488469
9	81.64130973475359	4.702607656639307	9.9545815597059	3.7015010489012
10	47.74367434503079	23.731780009097267	15.269421644704236	13.25512400116771
11	41.23817864586516	21.563779303040796	22.96679497885225	14.231247072241798
12	38.104238375527	19.814524396287773	25.70317114401515	16.37806608417008
13	21.53377190302586	37.22411182848259	26.05660671975668	15.185509548734869
14	15.40913935789595	39.50521734997997	27.81848917493228	17.267154117191797
15	13.099791577560982	22.06643719831361	47.093423491313466	17.740347732811937
16	14.714081074292077	17.958410558259843	44.91632551918912	22.41118284825896
17	15.608057190574145	20.170132453478345	29.7897445297596	34.43206582618791
18	20.829887913535238	22.854776404135862	34.00422276081658	22.31111292151232
19	26.46978553534695	27.01100497634032	24.690794788760122	21.828414699552603
20	29.036165027122074	23.859820634500366	24.28902150077734	22.814992837600222
21	20.905924764251818	30.234560106451593	26.207594180465314	22.651920948831272
22	21.957813125861357	25.175122371806623	24.915375058555163	27.95168944377686
23	18.944581356035766	30.972660678764676	23.811075581987414	26.27168238321215
24	19.547173397964656	24.41122358228613	36.18851707774089	19.853085942008324
25	17.991676680448347	26.574879325444513	32.525170234288545	22.9082737598186
26	17.675037509250018	32.0288940032723	26.83462663869597	23.461441848781714
27	19.253481062071867	29.22191219101543	27.60056212957494	23.92404461733776
28	17.392614920874152	27.14447680536603	35.82177505312396	19.641133220635858
29	17.220853106309022	23.22654229210371	34.64972131136411	24.902883290223155
30	19.71567649035622	27.68270908436696	31.416525794822704	21.185088630454114
31	27.18330991126771	26.166452813024026	23.289544254126017	23.360693021582247
32	28.53174199067191	25.789119941343003	24.384882244716458	21.294255823268635
33	25.08781577357312	26.079553555062223	24.36505835149392	24.467572319870737
34	19.748535272273028	25.592646150294986	28.81457191931947	25.844246658112517
35	20.24223168156853	25.047624866765787	28.512053877539937	26.198089574125742
36	28.43656014718562	22.949550907350456	26.51051956251655	22.103369382947378
37	20.391861341371513	29.524430232794963	28.395418779744325	21.688289646089192
38	22.23589075133913	29.135691833506456	26.362926604072044	22.26549081108237
39	20.78725296509773	30.10869196249754	26.244933705370784	22.859121367033953
40	24.38420334426363	24.368181293576924	27.033137131102464	24.21447823105698
41	18.340224172929524	23.08139337528938	29.010909930276924	29.567472521504172
42	22.940046301010884	26.753430144537905	24.14251478305736	26.16400877139385
43	22.52062160125461	26.01858829439839	26.433939591437706	25.02685051290929
44	22.563663889963813	28.626380713795935	27.04087659626469	21.76907879997556
45	18.48075656666463	31.664052899923284	24.779730748080407	25.075459785331677
46	23.264560717462	28.980359409899727	26.543378344433354	21.21170152820492
47	22.02814721277419	26.437334093701843	27.377611220866683	24.156907472657284
48	23.376986632450084	22.90963156072425	27.920731583127967	25.7926502236977
49	21.76201823526616	21.95794890595192	29.957025601336074	26.32300725744584
50	20.191993048059363	27.399336035357138	28.243073518130036	24.165597398453464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1175.0
1	1078.0
2	981.0
3	3943.0
4	6905.0
5	4891.0
6	2877.0
7	3170.0
8	3463.0
9	3726.5
10	3990.0
11	4138.5
12	4287.0
13	4208.5
14	4130.0
15	3846.0
16	3562.0
17	3458.0
18	3354.0
19	3189.5
20	3025.0
21	2867.0
22	2709.0
23	2690.0
24	2671.0
25	2797.0
26	2923.0
27	3375.5
28	3828.0
29	4741.5
30	5655.0
31	6632.5
32	7610.0
33	9948.0
34	12286.0
35	14304.0
36	16322.0
37	17407.0
38	18492.0
39	23424.0
40	28356.0
41	39833.0
42	51310.0
43	59076.0
44	66842.0
45	69191.0
46	71540.0
47	72820.5
48	74101.0
49	74298.5
50	74496.0
51	65890.5
52	57285.0
53	48732.0
54	40179.0
55	36715.5
56	33252.0
57	30440.0
58	27628.0
59	26718.5
60	25809.0
61	22982.0
62	20155.0
63	17992.5
64	15830.0
65	13841.0
66	11852.0
67	10046.0
68	8240.0
69	7495.0
70	6750.0
71	5885.5
72	5021.0
73	4137.5
74	3254.0
75	2639.0
76	2024.0
77	1613.0
78	1202.0
79	895.0
80	588.0
81	433.5
82	279.0
83	215.0
84	151.0
85	104.0
86	57.0
87	39.0
88	21.0
89	17.0
90	13.0
91	8.0
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	736485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.008222798921054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.19072674165334	23.163565260741663
2	7.300915597412967	4.381750037665722
3	2.9923920655583762	2.6938910341499795
4	1.764553666366939	2.118044782439684
5	1.2004488257780541	1.8011667911325504
6	0.8952644389554358	1.6119176848875465
7	0.6792848835614176	1.426889249089511
8	0.5951491699159879	1.428749511154551
9	0.4620013600049077	1.2477455769988626
>10	6.20295825193299	44.16716518551457
>50	0.6664356634981851	12.598678285209541
>100	0.048055904984596456	2.3165667156025145
>500	9.067151883886124E-4	0.21046092958874146
>1k	9.067151883886124E-4	0.8334089558245833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4660	0.6327352220343931	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1466	0.1990536127687597	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	993	0.13482962993136316	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06476710319965784	0.0
2	0.0	0.0	0.0	0.2543161096288451	0.0
3	0.0	0.0	0.0	0.4533697223976048	0.0
4	0.0	0.0	0.0	0.6949225035133099	0.0
5	0.0	0.0	0.0	1.146798644914696	0.0
6	0.0	0.0	0.0	2.1189840933623905	0.0
7	0.0	0.0	0.0	2.7230697162875006	0.0
8	0.0	0.0	0.0	4.5685927072513355	0.0
9	0.0	0.0	0.0	5.69543167885293	0.0
10	0.0	0.0	0.0	7.236535706769317	0.0
11	0.0	0.0	0.0	8.011840023897296	0.0
12	0.0	0.0	0.0	8.778997535591357	0.0
13	0.0	0.0	0.0	9.209827762955118	0.0
14	0.0	0.0	0.0	9.398018968478652	0.0
15	0.0	0.0	0.0	9.552400931451421	0.0
16	0.0	0.0	0.0	9.84405656598573	0.0
17	0.0	0.0	0.0	10.133132378799298	0.0
18	0.0	0.0	0.0	10.649639843309776	0.0
19	0.0	0.0	0.0	10.833486085935219	0.0
20	0.0	0.0	0.0	11.082235211850886	0.0
21	0.0	0.0	0.0	11.306815481645927	0.0
22	0.0	0.0	0.0	11.530173730625878	0.0
23	0.0	0.0	0.0	11.79996877057917	0.0
24	0.0	0.0	0.0	11.993048059363055	0.0
25	0.0	0.0	0.0	12.167253915558362	0.0
26	0.0	0.0	0.0	12.326795521972613	0.0
27	0.0	0.0	0.0	12.533317039722466	0.0
28	0.0	0.0	0.0	12.699647650664984	0.0
29	0.0	0.0	0.0	12.874939747584811	0.0
30	0.0	0.0	0.0	13.140797164911708	0.0
31	0.0	0.0	0.0	13.325865428352241	0.0
32	0.0	0.0	0.0	13.498441923460764	0.0
33	0.0	0.0	0.0	13.668710157029675	0.0
34	0.0	0.0	0.0	13.852963739926814	0.0
35	0.0	0.0	0.0	14.067632063110587	0.0
36	0.0	0.0	0.0	14.24333150030211	0.0
37	0.0	0.0	0.0	14.431522705825644	0.0
38	0.0	0.0	0.0	14.631119438956667	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGT	30	2.5264726E-6	44.000004	12
ATTAGCG	30	2.5264726E-6	44.000004	1
TACTGGC	30	2.5264726E-6	44.000004	3
ACGTTAG	25	4.441277E-5	44.0	1
GACGTAG	20	7.854638E-4	44.0	1
AAGCGGT	20	7.854638E-4	44.0	3
ACCCTTA	20	7.854638E-4	44.0	31
ACGGGTA	75	0.0	44.0	5
TCTAGCG	40	8.305506E-9	44.0	1
TGCGCAA	25	4.441277E-5	44.0	1
TGCGAAC	20	7.854638E-4	44.0	37
TCATCGG	20	7.854638E-4	44.0	2
GTCCATA	25	4.441277E-5	44.0	33
TTACCTA	20	7.854638E-4	44.0	40
CGCATCG	35	1.4451325E-7	43.999996	21
GCTTGCG	35	1.4451325E-7	43.999996	1
GTCTCAC	280	0.0	43.214283	22
TCACGAC	275	0.0	43.2	25
CGGTCTA	265	0.0	43.16981	31
ACGGTCT	265	0.0	43.16981	30
>>END_MODULE