FastQCFastQC Report
Thu 26 May 2016
SRR1547221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547221_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2004047
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318461.589084487539464No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC27890.13916839275725568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT196200.042.845061
GTATACG650.040.6153831
TCACGAC3000.039.60000225
GTTTTTT220300.038.5773962
TATAGCG750.038.1333351
CAACCCG1850.038.05405423
TAATACG357.2929197E-637.7142871
CGGTCTA3150.037.71428731
AGTACGG2350.037.4468082
CTCACGA3250.037.2307724
ACGGGCT6150.036.8455285
TAGCATA11600.036.79310230
GTTGATC11700.036.47863416
GGTACCT12700.036.3779538
GTAGCAT11800.036.3559329
GCCAGTA11900.036.23529425
TTAGGGA19650.036.162854
GGCGATA9200.036.1086968
AGGGCGA21050.036.0570076
TACGTAG1100.036.0000041
Produced by FastQC (version 0.11.5)