##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547221_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2004047
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.455888509600822	31.0	31.0	33.0	30.0	34.0
2	31.855907072039727	31.0	31.0	34.0	30.0	34.0
3	31.934715602977374	33.0	31.0	34.0	30.0	34.0
4	35.64419996137815	37.0	35.0	37.0	33.0	37.0
5	35.55973188253569	37.0	35.0	37.0	33.0	37.0
6	35.59275156720376	37.0	35.0	37.0	33.0	37.0
7	36.03456356063506	37.0	35.0	37.0	35.0	37.0
8	36.036394356020594	37.0	35.0	37.0	35.0	37.0
9	37.76710775745279	39.0	38.0	39.0	35.0	39.0
10	37.1411438953278	39.0	37.0	39.0	33.0	39.0
11	36.94919530330377	39.0	37.0	39.0	33.0	39.0
12	36.665469422623325	39.0	35.0	39.0	32.0	39.0
13	36.60365949501185	39.0	35.0	39.0	32.0	39.0
14	37.7209052482302	40.0	36.0	41.0	33.0	41.0
15	37.8266602529781	40.0	36.0	41.0	33.0	41.0
16	37.83471345731911	40.0	36.0	41.0	33.0	41.0
17	37.73768080289534	40.0	36.0	41.0	33.0	41.0
18	37.60039060960147	39.0	36.0	41.0	33.0	41.0
19	37.46091833175569	39.0	36.0	41.0	33.0	41.0
20	37.21487719599391	39.0	35.0	41.0	32.0	41.0
21	37.127196118653906	39.0	35.0	41.0	32.0	41.0
22	37.06283684963476	39.0	35.0	41.0	32.0	41.0
23	36.95738622896569	38.0	35.0	41.0	32.0	41.0
24	36.87915752474867	38.0	35.0	41.0	32.0	41.0
25	36.69557300801827	38.0	35.0	41.0	31.0	41.0
26	36.60060367845664	38.0	35.0	40.0	31.0	41.0
27	36.520280212989015	38.0	35.0	40.0	31.0	41.0
28	36.541973316993065	38.0	35.0	40.0	31.0	41.0
29	36.407054325572204	38.0	35.0	40.0	31.0	41.0
30	36.30162665845661	38.0	35.0	40.0	31.0	41.0
31	36.10677194696532	38.0	35.0	40.0	30.0	41.0
32	35.74186483650333	38.0	35.0	40.0	29.0	41.0
33	35.44630889395309	38.0	35.0	40.0	26.0	41.0
34	35.10599801302065	38.0	34.0	40.0	24.0	41.0
35	34.7818988277221	38.0	34.0	40.0	23.0	41.0
36	34.61517419501639	38.0	34.0	40.0	22.0	41.0
37	34.553123754083614	38.0	34.0	40.0	22.0	41.0
38	34.48364684061801	38.0	34.0	40.0	22.0	41.0
39	34.362203082063445	38.0	34.0	40.0	21.0	41.0
40	34.23995345418545	37.0	33.0	40.0	21.0	41.0
41	34.19527785525988	37.0	33.0	40.0	21.0	41.0
42	33.99675456713341	37.0	33.0	40.0	20.0	41.0
43	33.992043599775855	37.0	33.0	40.0	20.0	41.0
44	33.71723068371151	36.0	33.0	40.0	18.0	41.0
45	33.611835949955264	36.0	33.0	40.0	19.0	41.0
46	33.5910609880906	36.0	33.0	40.0	20.0	41.0
47	33.48662880660982	36.0	33.0	40.0	19.0	41.0
48	33.41973965680446	36.0	33.0	40.0	20.0	41.0
49	33.387379637303916	35.0	33.0	40.0	20.0	41.0
50	32.91868953173254	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	1.0
13	7.0
14	18.0
15	60.0
16	146.0
17	356.0
18	827.0
19	1623.0
20	3029.0
21	5070.0
22	7773.0
23	11718.0
24	17522.0
25	27367.0
26	40952.0
27	50911.0
28	50984.0
29	49138.0
30	50582.0
31	58261.0
32	70213.0
33	88691.0
34	138469.0
35	191706.0
36	166310.0
37	208763.0
38	296500.0
39	467045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.64451731920459	22.198281776824597	25.8647127537428	20.292488150228014
2	38.64335517081186	23.347456421930225	25.78697006607131	12.22221834118661
3	27.912768512914116	23.688765782439233	35.41718332953269	12.981282375113956
4	24.125082894762446	26.202529182199818	35.21454337148779	14.45784455154994
5	21.4886676809476	29.797055657876285	34.52923010288681	14.185046558289303
6	18.553506978628747	38.68776530690148	32.250291535078766	10.508436179391003
7	81.84907838987809	3.3556099233201615	12.940315272047012	1.8549964147547437
8	81.92058369888531	2.787659171666134	12.645312210741563	2.6464449187069965
9	76.8830770935013	4.588215745439104	14.99730295746557	3.5314042035940276
10	37.64936650687334	26.353773140051107	21.64525083493551	14.351609518140043
11	30.420943221391518	24.6199315684712	28.497535237447025	16.46158997269026
12	26.639993972197257	20.583748784334897	33.63279404125751	19.143463202210327
13	23.111384114244828	23.459529641769876	34.961006403542434	18.468079840442865
14	19.62518843120945	26.423432184973706	35.287445853315816	18.66393353050103
15	19.28567543575575	21.789409130624183	39.735395427352756	19.18952000626732
16	21.414168430181526	20.5545578521861	37.85759515620143	20.173678561430943
17	20.84851303387595	21.06597300362716	34.100148349814155	23.985365612682735
18	23.37365341232017	21.775337604357585	34.42958174134639	20.421427241975863
19	25.61656488096337	25.228899322221487	29.95952689732327	19.195008899491878
20	26.42133642574251	23.724044396164363	29.437932343902112	20.416686834191015
21	24.687195459986714	25.188830401682193	31.216483445747528	18.907490692583558
22	23.576642663570265	21.826084917170103	31.012346516823207	23.58492590243642
23	21.910015084476562	25.81551231083902	30.088266392953855	22.186206211730564
24	22.04109983448492	24.00437714285144	33.32870935661688	20.625813666046756
25	22.58619683071305	23.699593871800413	31.92035915325339	21.793850144233147
26	20.071585147454126	25.414723307387497	31.624956899713432	22.88873464544494
27	21.399597913621786	23.15818940374153	32.330479275186654	23.111733407450025
28	21.52933538983866	24.332263664474933	33.83613258571281	20.302268359973592
29	22.435801156360107	23.528490100282077	32.6782256104772	21.357483132880617
30	23.22380662728968	22.944721356335453	33.62376231695165	20.20770969942322
31	26.746777894929608	22.669777704814308	29.287636467607797	21.295807932648287
32	26.211261512329802	23.414620515387114	29.769611191753487	20.6045067805296
33	25.827038986610596	22.974760571982593	28.862995728144103	22.335204713262712
34	23.862913394745732	24.815286268236225	30.27319219559222	21.048608141425827
35	24.346983878122618	24.48011448833286	29.929936772939957	21.242964860604566
36	27.389627089584227	25.130348739325974	26.6281679022498	20.851856268840002
37	25.283339163203255	25.81067210499554	27.914315382822856	20.991673348978342
38	25.094870529483586	26.73076030651976	26.62327779737701	21.551091366619644
39	24.34543700821388	25.01208803985136	26.755011234766453	23.887463717168313
40	24.29738424298432	23.583079638351794	29.29367425015481	22.825861868509072
41	21.96520341089805	23.88172532879718	29.36572844848449	24.78734281182028
42	23.51381978566371	25.70249100944239	26.931504101450717	23.852185103443183
43	23.03164546540076	24.389697447215557	28.472635621819247	24.10602146556443
44	23.644156050232354	25.078503647868537	29.06433831142683	22.21300199047228
45	22.43240802236674	25.650695817014274	28.886897363185593	23.029998797433393
46	24.067449515904567	25.28543492243445	28.444692165403307	22.20242339625767
47	23.13363908131895	25.22515689502292	28.886697767068338	22.754506256589792
48	23.258187058487152	24.690388997862826	29.359041978556395	22.692381965093634
49	23.535326267298124	23.858971371429913	29.171870719598893	23.433831641673073
50	22.257412126561903	24.366344701496523	29.947301635141294	23.428941536800284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1956.0
1	2337.0
2	2718.0
3	27220.5
4	51723.0
5	35483.5
6	19244.0
7	19383.0
8	19522.0
9	19994.5
10	20467.0
11	20431.0
12	20395.0
13	19585.0
14	18775.0
15	17819.0
16	16863.0
17	15882.5
18	14902.0
19	13904.0
20	12906.0
21	12310.5
22	11715.0
23	11852.0
24	11989.0
25	12601.5
26	13214.0
27	14662.5
28	16111.0
29	17969.5
30	19828.0
31	23136.5
32	26445.0
33	29523.0
34	32601.0
35	38309.0
36	44017.0
37	46319.0
38	48621.0
39	56589.5
40	64558.0
41	72446.5
42	80335.0
43	90567.0
44	100799.0
45	107791.0
46	114783.0
47	126205.5
48	137628.0
49	151000.0
50	164372.0
51	161338.0
52	158304.0
53	143326.0
54	128348.0
55	117375.0
56	106402.0
57	103772.0
58	101142.0
59	98663.5
60	96185.0
61	91689.5
62	87194.0
63	80031.0
64	72868.0
65	61894.0
66	50920.0
67	43523.5
68	36127.0
69	32558.0
70	28989.0
71	25333.0
72	21677.0
73	17594.5
74	13512.0
75	10587.5
76	7663.0
77	5858.0
78	4053.0
79	3074.0
80	2095.0
81	1597.5
82	1100.0
83	839.0
84	578.0
85	420.5
86	263.0
87	165.5
88	68.0
89	56.0
90	44.0
91	32.0
92	20.0
93	12.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2004047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.195786289151577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4372898239959	23.221297852930135
2	7.344333695728478	4.582245288163208
3	3.315712513554505	3.1030877690733583
4	2.115801301772226	2.6401634096158033
5	1.5085640535387168	2.3530420908845016
6	1.1462963201129484	2.1455769015770656
7	0.9950725134448937	2.172944863014507
8	0.8371347455777894	2.0892061294614397
9	0.7268800956727836	2.040803651020159
>10	7.277910101244754	45.72592880432262
>50	0.254821657797941	5.011524610132287
>100	0.035682880374123364	2.0903278385880473
>500	0.003214497989320012	0.725266365322295
>1k	0.001125074296262004	0.5018452160740305
>5k	0.0	0.0
>10k+	1.607248994660006E-4	1.596739209820519
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31846	1.589084487539464	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	2789	0.13916839275725568	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.989902931418275E-5	0.0	0.0	0.04211478074117024	0.0
2	4.989902931418275E-5	0.0	0.0	0.15788052875007422	0.0
3	4.989902931418275E-5	0.0	0.0	0.3397125915709562	0.0
4	4.989902931418275E-5	0.0	0.0	0.46905087555331787	0.0
5	4.989902931418275E-5	0.0	0.0	0.7301724959544362	0.0
6	4.989902931418275E-5	0.0	0.0	1.4105956596826321	0.0
7	4.989902931418275E-5	0.0	0.0	1.829947102039024	0.0
8	4.989902931418275E-5	0.0	0.0	2.9395019178691917	0.0
9	4.989902931418275E-5	0.0	0.0	3.7255114276261985	0.0
10	4.989902931418275E-5	0.0	0.0	4.751435470325796	0.0
11	4.989902931418275E-5	0.0	0.0	5.267092039258561	0.0
12	4.989902931418275E-5	0.0	0.0	5.760593439175827	0.0
13	4.989902931418275E-5	0.0	0.0	6.1186688735344035	0.0
14	4.989902931418275E-5	0.0	0.0	6.31641872670651	0.0
15	4.989902931418275E-5	0.0	0.0	6.447353779626925	0.0
16	4.989902931418275E-5	0.0	0.0	6.6252438191319865	0.0
17	4.989902931418275E-5	0.0	0.0	6.789661120722219	0.0
18	4.989902931418275E-5	0.0	0.0	7.101829448111746	0.0
19	4.989902931418275E-5	0.0	0.0	7.230419246654395	0.0
20	4.989902931418275E-5	0.0	0.0	7.395734730772283	0.0
21	4.989902931418275E-5	0.0	0.0	7.537298276936618	0.0
22	4.989902931418275E-5	0.0	0.0	7.686446475556711	0.0
23	4.989902931418275E-5	0.0	0.0	7.854506406286879	0.0
24	4.989902931418275E-5	0.0	0.0	8.005051777727768	0.0
25	4.989902931418275E-5	0.0	0.0	8.133092686947961	0.0
26	9.97980586283655E-5	0.0	0.0	8.267321075803112	0.0
27	9.97980586283655E-5	0.0	0.0	8.449103239594681	0.0
28	9.97980586283655E-5	0.0	0.0	8.588620925557136	0.0
29	9.97980586283655E-5	0.0	0.0	8.725693559083195	0.0
30	9.97980586283655E-5	0.0	0.0	8.921247854965477	0.0
31	9.97980586283655E-5	0.0	0.0	9.069497871057914	0.0
32	9.97980586283655E-5	0.0	0.0	9.247138415416405	0.0
33	9.97980586283655E-5	0.0	0.0	9.403022982993912	0.0
34	9.97980586283655E-5	0.0	0.0	9.554017445698628	0.0
35	9.97980586283655E-5	0.0	0.0	9.719931718168286	0.0
36	9.97980586283655E-5	0.0	0.0	9.876065780892365	0.0
37	9.97980586283655E-5	0.0	0.0	10.044574802886359	0.0
38	9.97980586283655E-5	0.0	0.0	10.23533879195448	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	19620	0.0	42.84506	1
GTATACG	65	0.0	40.615383	1
TCACGAC	300	0.0	39.600002	25
GTTTTTT	22030	0.0	38.577396	2
TATAGCG	75	0.0	38.133335	1
CAACCCG	185	0.0	38.054054	23
TAATACG	35	7.2929197E-6	37.714287	1
CGGTCTA	315	0.0	37.714287	31
AGTACGG	235	0.0	37.446808	2
CTCACGA	325	0.0	37.23077	24
ACGGGCT	615	0.0	36.845528	5
TAGCATA	1160	0.0	36.793102	30
GTTGATC	1170	0.0	36.478634	16
GGTACCT	1270	0.0	36.377953	8
GTAGCAT	1180	0.0	36.35593	29
GCCAGTA	1190	0.0	36.235294	25
TTAGGGA	1965	0.0	36.16285	4
GGCGATA	920	0.0	36.108696	8
AGGGCGA	2105	0.0	36.057007	6
TACGTAG	110	0.0	36.000004	1
>>END_MODULE