FastQCFastQC Report
Thu 26 May 2016
SRR1547220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547220_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3375980
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330500.978974993927689No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39990.11845449321382237No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34090.10097808636307087No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC254.4446788E-544.021
CGTTTTT251650.043.064571
CGGTCTA3800.039.3684231
TAATGCG1300.038.9230771
TCACGAC3750.038.71999725
GTTTTTT284100.038.718762
TACGGGA13000.038.2461554
CCGTAAG700.037.7142871
AGTACGG5850.037.230772
GACGGTC3850.037.14285729
TAGGGCG13900.036.7194255
ACTACCG301.3016294E-436.66666435
AACGGGA12100.036.5454564
AGGGCGA36900.036.4281856
ACGGGTA2900.036.413795
ACGGTCT3950.036.20253430
GCGTTAG3100.036.1935461
TTAGGGA46550.036.1546754
ACGGGAT14400.035.755
TATACTA22500.035.5911144
Produced by FastQC (version 0.11.5)