##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547220_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3375980
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303698481626075	31.0	31.0	33.0	30.0	34.0
2	31.687658990870798	31.0	31.0	34.0	30.0	34.0
3	31.78634440962328	31.0	31.0	34.0	30.0	34.0
4	35.486262359374166	37.0	35.0	37.0	33.0	37.0
5	35.36566626579541	37.0	35.0	37.0	33.0	37.0
6	35.39481395031961	37.0	35.0	37.0	33.0	37.0
7	35.952888050284656	37.0	35.0	37.0	35.0	37.0
8	35.956670951842135	37.0	35.0	37.0	35.0	37.0
9	37.660710667717225	39.0	37.0	39.0	35.0	39.0
10	36.9937810650537	39.0	37.0	39.0	32.0	39.0
11	36.72201582947766	39.0	35.0	39.0	32.0	39.0
12	36.24434534564778	38.0	35.0	39.0	32.0	39.0
13	36.031579274758734	38.0	35.0	39.0	31.0	39.0
14	37.07779044899555	39.0	35.0	41.0	31.0	41.0
15	37.29214183733316	39.0	35.0	41.0	32.0	41.0
16	37.362996522491244	39.0	35.0	41.0	32.0	41.0
17	37.30434392383841	39.0	35.0	41.0	32.0	41.0
18	37.199271618907694	39.0	35.0	41.0	32.0	41.0
19	37.11193816314078	38.0	35.0	41.0	32.0	41.0
20	36.892044976569764	38.0	35.0	41.0	32.0	41.0
21	36.76647877060883	38.0	35.0	41.0	31.0	41.0
22	36.68356299504144	38.0	35.0	40.0	31.0	41.0
23	36.58709648753843	38.0	35.0	40.0	31.0	41.0
24	36.505013062873594	38.0	35.0	40.0	31.0	41.0
25	36.331244260925715	38.0	35.0	40.0	31.0	41.0
26	36.21898559825591	38.0	35.0	40.0	31.0	41.0
27	36.094723902392786	38.0	35.0	40.0	30.0	41.0
28	36.14589334060036	38.0	35.0	40.0	30.0	41.0
29	36.087359818482334	38.0	35.0	40.0	30.0	41.0
30	35.99880686496958	38.0	35.0	40.0	30.0	41.0
31	35.83032867493291	38.0	35.0	40.0	30.0	41.0
32	35.472898832339055	38.0	34.0	40.0	28.0	41.0
33	35.288034289302665	38.0	34.0	40.0	26.0	41.0
34	35.10720650003851	38.0	34.0	40.0	25.0	41.0
35	34.876212536804125	38.0	34.0	40.0	24.0	41.0
36	34.67612426613902	38.0	34.0	40.0	23.0	41.0
37	34.641741657237304	38.0	34.0	40.0	23.0	41.0
38	34.60976309101357	38.0	34.0	40.0	23.0	41.0
39	34.53195575803174	37.0	34.0	40.0	23.0	41.0
40	34.438230084301445	37.0	34.0	40.0	23.0	41.0
41	34.460141055338006	37.0	34.0	40.0	23.0	41.0
42	34.23821586620774	37.0	33.0	40.0	22.0	41.0
43	34.250761260434004	37.0	33.0	40.0	22.0	41.0
44	33.90315167743885	37.0	33.0	40.0	20.0	41.0
45	33.82688967351702	36.0	33.0	40.0	20.0	41.0
46	33.84573042494328	36.0	33.0	40.0	20.0	41.0
47	33.771612391068665	36.0	33.0	40.0	20.0	41.0
48	33.74051238455204	36.0	33.0	40.0	20.0	41.0
49	33.74182341127613	36.0	33.0	40.0	20.0	41.0
50	33.149107814619754	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	5.0
13	15.0
14	33.0
15	95.0
16	291.0
17	760.0
18	1848.0
19	3695.0
20	6325.0
21	9793.0
22	14849.0
23	21815.0
24	31831.0
25	46954.0
26	66335.0
27	78053.0
28	79547.0
29	81816.0
30	90592.0
31	106530.0
32	129588.0
33	164669.0
34	255731.0
35	401512.0
36	250429.0
37	303090.0
38	446923.0
39	782852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.839371086321606	23.96018341340885	28.257691100065756	16.942754400203793
2	33.57590980989224	25.05322898832339	28.334113353752095	13.036747848032276
3	27.26778594659921	24.79520020853204	34.809122091955516	13.127891752913229
4	23.832724127512602	27.701171215469284	34.566940562444096	13.89916409457402
5	21.814524967564974	31.87646253828518	32.6373378989212	13.671674595228644
6	18.457277590507054	40.30746627645899	30.51454688712611	10.720709245907853
7	83.27747794714423	3.825496596543818	10.874501626194467	2.022523830117477
8	83.19430209894608	3.0915467508693775	10.178851770448876	3.5352993797356618
9	77.33031593789063	5.758120604979887	13.309231689761194	3.60233176736829
10	39.79448930384659	31.777350576721425	16.95697249391288	11.471187625519107
11	33.51684547894241	23.419007221606762	29.47979549641882	13.584351803032007
12	31.26155960639577	21.553859916231733	30.427549926243643	16.757030551128857
13	21.58750940467657	30.8491460257466	31.925988898038494	15.637355671538339
14	17.087897440150712	33.589861314344276	31.51603386275985	17.80620738274516
15	15.543190421744205	23.340007938435654	44.84570406222786	16.271097577592283
16	17.12062867671017	21.954425085456666	41.71846397194296	19.2064822658902
17	17.678096434220585	22.391453740839697	32.89216760762801	27.038282217311714
18	20.753914418924282	23.309735247246728	35.74775324498368	20.188597088845313
19	24.39534594399256	27.14770229681455	29.287199568717824	19.169752190475062
20	26.1246512123887	25.110190226245415	28.562432241897167	20.202726319468717
21	21.913636929128728	28.580856521661858	29.526389374344635	19.97911717486478
22	21.451193431240707	25.23501324059977	28.826207501229273	24.487585826930257
23	18.775970236790503	29.705004176565026	28.29702782599423	23.22199776065024
24	19.231097340624057	24.76128413083016	36.70803736988964	19.299581158656153
25	18.33381714346649	26.401637450458832	33.765691739879976	21.498853666194705
26	17.899424759625354	30.174764068507514	30.654654352217726	21.271156819649406
27	19.49380624292798	28.242169681100005	30.685371358835063	21.57865271713695
28	18.489149817238253	26.71698292051493	35.94088235119876	18.85298491104805
29	18.52620572396756	24.122595513006594	34.20357940509126	23.147619357934584
30	20.643783434735987	27.155699974525916	31.48955266322668	20.71096392751142
31	25.667332152441663	25.74043685092921	26.554008021374536	22.03822297525459
32	26.441003797415863	26.212122109728135	26.33504937825461	21.01182471460139
33	24.618718120368012	28.09806337715271	25.61505103703221	21.66816746544707
34	21.17145243751444	27.741574298425935	27.72421637568943	23.362756888370193
35	22.10164159740283	27.049952902564588	28.0987446608096	22.749660839222983
36	26.190232169621858	26.972642018021432	26.813073537165504	20.024052275191202
37	21.94776035403053	28.960983181179984	27.3598777243941	21.73137874039538
38	20.76019407697913	29.72304338295843	27.648712373888472	21.86805016617397
39	20.5468041872286	28.169509298040868	27.257655554831484	24.026030959899053
40	22.691633244272776	25.65515790970326	28.24930834898311	23.40390049704086
41	18.95896895123786	24.50932766189373	27.92723890544375	28.604464481424653
42	22.51595092387988	26.2838642409019	26.061854631840237	25.138330203377983
43	21.952588581685912	25.715969881338157	28.414741793494038	23.916699743481892
44	22.485915200919436	28.484410452668556	27.950284065663894	21.079390280748108
45	19.596650454090366	30.064870052547704	27.327116866806083	23.011362626555844
46	23.060207702652267	28.601976315025563	26.732060024052274	21.605755958269896
47	21.833097352472468	25.807795069876004	27.83908672444742	24.520020853204105
48	22.908548036422015	23.730383473835744	29.117796906379777	24.24327158336246
49	22.311388100640407	22.807155255659097	29.40633534558854	25.475121298111958
50	20.53513350197572	26.858304847777536	29.66344587349451	22.943115776752233
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4704.0
1	4244.0
2	3784.0
3	27760.0
4	51736.0
5	37136.0
6	22536.0
7	23438.5
8	24341.0
9	25856.5
10	27372.0
11	28186.5
12	29001.0
13	28476.0
14	27951.0
15	26977.5
16	26004.0
17	24629.5
18	23255.0
19	22005.5
20	20756.0
21	19910.5
22	19065.0
23	19318.0
24	19571.0
25	19896.0
26	20221.0
27	22510.5
28	24800.0
29	28968.5
30	33137.0
31	40504.5
32	47872.0
33	56447.5
34	65023.0
35	73931.5
36	82840.0
37	92932.0
38	103024.0
39	119539.0
40	136054.0
41	171261.0
42	206468.0
43	238222.0
44	269976.0
45	276344.0
46	282712.0
47	293317.0
48	303922.0
49	322536.0
50	341150.0
51	325182.5
52	309215.0
53	267565.0
54	225915.0
55	192405.0
56	158895.0
57	139984.0
58	121073.0
59	112307.0
60	103541.0
61	90851.0
62	78161.0
63	66858.5
64	55556.0
65	45442.0
66	35328.0
67	29428.5
68	23529.0
69	20636.0
70	17743.0
71	14784.0
72	11825.0
73	9847.0
74	7869.0
75	6633.0
76	5397.0
77	3760.5
78	2124.0
79	1875.5
80	1627.0
81	1097.0
82	567.0
83	366.0
84	165.0
85	138.5
86	112.0
87	71.5
88	31.0
89	25.5
90	20.0
91	13.0
92	6.0
93	6.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3375980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.357729270567926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.68022513465537	19.95151847911925
2	8.033586543487678	4.074270252828762
3	2.969751591854576	2.259184706012594
4	1.5501502253552029	1.5723315897306838
5	1.022173235236304	1.295999608337137
6	0.7132596433166942	1.0851986960907947
7	0.5295127828728667	0.9399069255376621
8	0.43113667981093307	0.8746117764205736
9	0.3550966522916844	0.8104000296327775
>10	4.001290666401663	23.407721735661692
>50	1.1556323696533715	20.68837619571787
>100	0.551437692464487	20.274936917368706
>500	0.004497855066092478	0.7332260916599895
>1k	0.0021305629260437906	1.0403352393887082
>5k	0.0	0.0
>10k+	1.1836460700243281E-4	0.9919817564927961
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33050	0.978974993927689	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3999	0.11845449321382237	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3409	0.10097808636307087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07420067654429233	0.0
2	0.0	0.0	0.0	0.26809992950195205	0.0
3	0.0	0.0	0.0	0.4830004917090741	0.0
4	0.0	0.0	0.0	0.7712723416607918	0.0
5	0.0	0.0	0.0	1.2735857439913743	0.0
6	0.0	0.0	0.0	2.386240439813032	0.0
7	0.0	0.0	0.0	3.1408361423942086	0.0
8	0.0	0.0	0.0	5.3263052506235224	0.0
9	0.0	0.0	0.0	6.6165083916373915	0.0
10	0.0	0.0	0.0	8.289296737539914	0.0
11	2.962102856059574E-5	0.0	0.0	9.067915094283734	0.0
12	2.962102856059574E-5	0.0	0.0	9.901806290321625	0.0
13	2.962102856059574E-5	0.0	0.0	10.386021244201684	0.0
14	2.962102856059574E-5	0.0	0.0	10.609867357034105	0.0
15	2.962102856059574E-5	0.0	0.0	10.791029567710709	0.0
16	2.962102856059574E-5	6.516626283331063E-4	0.0	11.105693754109918	0.0
17	2.962102856059574E-5	6.516626283331063E-4	0.0	11.41807712130996	0.0
18	2.962102856059574E-5	6.516626283331063E-4	0.0	11.978062666248023	0.0
19	2.962102856059574E-5	6.516626283331063E-4	0.0	12.183484499315755	0.0
20	2.962102856059574E-5	6.516626283331063E-4	0.0	12.460974294871415	0.0
21	5.924205712119148E-5	6.516626283331063E-4	0.0	12.715211583007008	0.0
22	5.924205712119148E-5	6.516626283331063E-4	0.0	12.982097050337975	0.0
23	5.924205712119148E-5	6.516626283331063E-4	0.0	13.284290783713173	0.0
24	8.886308568178722E-5	6.516626283331063E-4	0.0	13.521673706597788	0.0
25	1.1848411424238295E-4	6.516626283331063E-4	0.0	13.722800490524232	0.0
26	1.1848411424238295E-4	6.516626283331063E-4	0.0	13.921913044508557	0.0
27	1.1848411424238295E-4	6.516626283331063E-4	0.0	14.145462947055373	0.0
28	1.1848411424238295E-4	6.516626283331063E-4	0.0	14.360363509262495	0.0
29	1.1848411424238295E-4	6.516626283331063E-4	0.0	14.583972653866432	0.0
30	1.1848411424238295E-4	6.516626283331063E-4	0.0	14.910751840946926	0.0
31	1.1848411424238295E-4	6.516626283331063E-4	0.0	15.155569642000248	0.0
32	1.1848411424238295E-4	6.516626283331063E-4	0.0	15.38282217311714	0.0
33	1.1848411424238295E-4	6.516626283331063E-4	0.0	15.598018945609867	0.0
34	1.1848411424238295E-4	6.516626283331063E-4	0.0	15.828470547811301	0.0
35	1.1848411424238295E-4	6.516626283331063E-4	0.0	16.09837736005545	0.0
36	1.1848411424238295E-4	6.516626283331063E-4	0.0	16.333272116540975	0.0
37	1.1848411424238295E-4	6.516626283331063E-4	0.0	16.57803067553718	0.0
38	1.1848411424238295E-4	6.516626283331063E-4	0.0	16.823766728475878	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATC	25	4.4446788E-5	44.0	21
CGTTTTT	25165	0.0	43.06457	1
CGGTCTA	380	0.0	39.36842	31
TAATGCG	130	0.0	38.923077	1
TCACGAC	375	0.0	38.719997	25
GTTTTTT	28410	0.0	38.71876	2
TACGGGA	1300	0.0	38.246155	4
CCGTAAG	70	0.0	37.714287	1
AGTACGG	585	0.0	37.23077	2
GACGGTC	385	0.0	37.142857	29
TAGGGCG	1390	0.0	36.719425	5
ACTACCG	30	1.3016294E-4	36.666664	35
AACGGGA	1210	0.0	36.545456	4
AGGGCGA	3690	0.0	36.428185	6
ACGGGTA	290	0.0	36.41379	5
ACGGTCT	395	0.0	36.202534	30
GCGTTAG	310	0.0	36.193546	1
TTAGGGA	4655	0.0	36.154675	4
ACGGGAT	1440	0.0	35.75	5
TATACTA	2250	0.0	35.59111	44
>>END_MODULE