FastQCFastQC Report
Thu 26 May 2016
SRR1547219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547219_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2061035
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221131.0729075440252107No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC28530.1384255968481855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124650.042.252711
TCACGAC4750.041.68420825
TAGCCGT3250.041.2923144
CGACGGT4950.040.8888928
CGGTCTA5000.040.4831
TCGTTAG603.6379788E-1240.3333321
TACCCGT404.1266685E-738.536
CATATGC19350.038.2015533
CACGACG5250.038.13333526
TACGGGA4350.037.9310384
CGACGTT3350.037.43283527
TCGACGT3350.037.43283526
GTTTTTT145900.037.2446862
TTGATCC20200.037.13861517
CTCACGA5350.037.0093524
TCTCACG5350.037.0093523
GTAGCAT20200.036.81188229
GTTGATC20550.036.72019616
GGTACCT21350.036.6838388
CGCGTAG301.3014534E-436.6666641
Produced by FastQC (version 0.11.5)