##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547219_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2061035
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.358472320945545	31.0	31.0	33.0	30.0	34.0
2	31.749373494385104	31.0	31.0	34.0	30.0	34.0
3	31.866226919969822	31.0	31.0	34.0	30.0	34.0
4	35.56318936844838	37.0	35.0	37.0	33.0	37.0
5	35.46244047286921	37.0	35.0	37.0	33.0	37.0
6	35.48828331396604	37.0	35.0	37.0	33.0	37.0
7	35.95331762924938	37.0	35.0	37.0	35.0	37.0
8	35.942222718197414	37.0	35.0	37.0	35.0	37.0
9	37.656736542562356	39.0	37.0	39.0	35.0	39.0
10	37.04659940272727	39.0	37.0	39.0	33.0	39.0
11	36.83330511126691	39.0	37.0	39.0	32.0	39.0
12	36.44396383370491	39.0	35.0	39.0	32.0	39.0
13	36.32693040147305	39.0	35.0	39.0	32.0	39.0
14	37.42071871656716	40.0	35.0	41.0	32.0	41.0
15	37.56540718619529	40.0	35.0	41.0	32.0	41.0
16	37.60693438005662	40.0	35.0	41.0	32.0	41.0
17	37.510886520607365	39.0	35.0	41.0	32.0	41.0
18	37.42818729424779	39.0	36.0	41.0	32.0	41.0
19	37.36106713374591	39.0	36.0	41.0	32.0	41.0
20	37.158271936187404	39.0	35.0	41.0	32.0	41.0
21	37.04184887689923	39.0	35.0	41.0	32.0	41.0
22	36.98045059884961	39.0	35.0	41.0	32.0	41.0
23	36.87254753073092	38.0	35.0	41.0	31.0	41.0
24	36.80168410531602	38.0	35.0	41.0	31.0	41.0
25	36.60654719594767	38.0	35.0	41.0	31.0	41.0
26	36.51216937121398	38.0	35.0	40.0	31.0	41.0
27	36.42222378562227	38.0	35.0	40.0	31.0	41.0
28	36.45862976611266	38.0	35.0	40.0	31.0	41.0
29	36.32655437680583	38.0	35.0	40.0	30.0	41.0
30	36.273136069984254	38.0	35.0	40.0	30.0	41.0
31	36.129134633812626	38.0	35.0	40.0	30.0	41.0
32	35.888402186280196	38.0	35.0	40.0	29.0	41.0
33	35.70462655898614	38.0	35.0	40.0	29.0	41.0
34	35.53597876794911	38.0	35.0	40.0	27.0	41.0
35	35.24516808302624	38.0	34.0	40.0	26.0	41.0
36	35.08299519416216	38.0	34.0	40.0	25.0	41.0
37	35.02028883546374	38.0	34.0	40.0	25.0	41.0
38	34.97008978498667	38.0	34.0	40.0	25.0	41.0
39	34.8547448248089	37.0	34.0	40.0	24.0	41.0
40	34.76347660277482	37.0	34.0	40.0	24.0	41.0
41	34.73479344115942	37.0	34.0	40.0	24.0	41.0
42	34.5407457903432	37.0	34.0	40.0	23.0	41.0
43	34.53506757527165	37.0	34.0	40.0	24.0	41.0
44	34.267267174016936	36.0	33.0	40.0	23.0	41.0
45	34.151035280817645	36.0	33.0	40.0	23.0	41.0
46	34.12675088001902	36.0	33.0	40.0	23.0	41.0
47	33.999371674910904	36.0	33.0	40.0	23.0	41.0
48	33.94208880489657	35.0	33.0	40.0	23.0	41.0
49	33.89588240859568	35.0	33.0	40.0	23.0	41.0
50	33.358400997557055	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	7.0
13	6.0
14	22.0
15	61.0
16	161.0
17	368.0
18	907.0
19	1768.0
20	3197.0
21	5186.0
22	8044.0
23	11444.0
24	16311.0
25	23954.0
26	33630.0
27	40879.0
28	44010.0
29	47089.0
30	52970.0
31	62404.0
32	76352.0
33	96392.0
34	151952.0
35	225730.0
36	170710.0
37	211663.0
38	303860.0
39	471951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.70343783584461	22.54605089190625	26.082866132792503	16.66764513945663
2	35.71220284953919	23.93287838391876	26.989740591498933	13.365178175043122
3	29.58382560218531	24.655330938096636	32.26742874332556	13.493414716392493
4	25.487825291661714	27.026033036799475	32.14865346779652	15.337488203742295
5	22.485256194096657	31.316207633543343	30.87094590824513	15.327590264114873
6	19.705633334708047	39.85366575531226	29.183298682458087	11.25740222752161
7	84.1402499229756	3.9639792628460944	9.490231849531911	2.4055389646464036
8	83.48630663719928	3.531478116577351	9.404449706094269	3.5777655401291097
9	78.36164839510246	5.101854165504225	12.37096895491828	4.1655284844750335
10	40.604793222822515	25.819842942987382	17.78203669515559	15.793327139034513
11	32.727488858753006	25.10408605385158	25.83692174077587	16.331503346619538
12	29.416628053380943	21.194593978268202	29.571307619715338	19.817470348635517
13	23.53482594909839	27.38876341255728	30.520296841150195	18.55611379719413
14	20.208341925294814	30.26484266400134	30.466197808382685	19.060617602321166
15	17.991979757743078	22.568515333315542	39.08773989767277	20.35176501126861
16	20.26142205251245	20.08694660692322	37.543418719235724	22.108212621328605
17	19.37026785086134	21.1780488929106	31.9516165421742	27.50006671405386
18	23.182333148151294	22.335525597575977	32.38275914770977	22.099382106562963
19	27.304339809852817	25.89524195367861	26.281504195707495	20.518914040761075
20	28.178124097844044	24.31001899531061	25.72396878267472	21.787888124170625
21	24.715397846227745	27.588371861710257	27.883223720121205	19.813006571940797
22	24.115456554595145	22.902231160557683	26.772810747997973	26.209501536849206
23	21.91903582423394	28.230427916071292	25.33552317161038	24.515013088084384
24	22.25939879720626	25.12043706196159	31.736530432525406	20.883633708306746
25	23.109408622366917	24.705111752105132	29.334048184528648	22.851431440999303
26	20.36981419529508	27.671194327122052	27.80185683406638	24.157134643516486
27	22.055278052046663	24.71733861870371	28.461040205527805	24.76634312372182
28	22.266240020184032	25.340181025552695	32.08150274012814	20.31207621413513
29	22.295545684571103	23.543753502487828	31.155705749781053	23.004995063160013
30	24.305312622056395	24.068392822053	30.305792963244194	21.320501592646412
31	27.49977559818247	23.714492961060827	26.275827436215298	22.509904004541408
32	28.640416101618847	23.841031326493727	25.93677448466426	21.581778087223167
33	26.444140929193345	23.77164871047799	26.376699085653566	23.4075112746751
34	24.906855050981665	25.589570288714164	27.610884822431448	21.89268983787272
35	23.732930299582492	24.694291945551626	29.58343744769012	21.989340307175766
36	29.06656121802881	24.89128035186205	24.86270247715347	21.179455952955674
37	24.185615479601267	28.258860232844178	26.185824112642436	21.369700174912122
38	25.996938431419164	27.765855504637234	24.682841387943437	21.554364676000166
39	23.65879279100064	27.847270910003953	24.412200666170154	24.081735632825254
40	24.97589803181411	24.520689847576584	27.85707181100758	22.64634030960173
41	22.049407215306875	24.35679161198136	27.619521259949494	25.974279912762277
42	24.610159458718556	26.35001346410905	24.45970107251939	24.580126004653003
43	23.52609247295655	25.001855863680138	27.044664452568735	24.427387210794578
44	24.555429674896352	26.37286606001354	27.246553309380968	21.825150955709148
45	21.531318002848085	27.039230289636034	27.71374576365758	23.715705943858303
46	25.533530483470685	25.438141516277017	26.877758019635767	22.150569980616535
47	23.568935025363473	26.233664154174967	26.705465943081997	23.49193487737957
48	24.487454118925687	24.258928159880835	27.33878852130119	23.91482919989229
49	24.45150130880844	23.77038720836861	27.43966017073946	24.338451312083492
50	22.480743898090036	24.706664370085903	28.89048463514691	23.922107096677156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2585.0
1	2878.5
2	3172.0
3	18513.5
4	33855.0
5	22890.0
6	11925.0
7	12305.5
8	12686.0
9	13125.5
10	13565.0
11	13634.0
12	13703.0
13	13117.0
14	12531.0
15	11810.0
16	11089.0
17	10541.5
18	9994.0
19	9582.0
20	9170.0
21	9070.5
22	8971.0
23	9350.5
24	9730.0
25	9776.0
26	9822.0
27	11449.0
28	13076.0
29	15334.5
30	17593.0
31	21219.5
32	24846.0
33	27062.5
34	29279.0
35	34909.5
36	40540.0
37	45567.0
38	50594.0
39	58685.0
40	66776.0
41	79474.5
42	92173.0
43	110029.0
44	127885.0
45	136475.0
46	145065.0
47	161354.5
48	177644.0
49	182497.0
50	187350.0
51	168600.0
52	149850.0
53	135406.0
54	120962.0
55	113829.5
56	106697.0
57	107686.5
58	108676.0
59	103181.5
60	97687.0
61	91105.5
62	84524.0
63	78334.0
64	72144.0
65	61639.0
66	51134.0
67	45168.0
68	39202.0
69	36103.0
70	33004.0
71	29515.0
72	26026.0
73	21382.5
74	16739.0
75	12954.5
76	9170.0
77	6827.0
78	4484.0
79	3517.5
80	2551.0
81	2019.5
82	1488.0
83	1023.0
84	558.0
85	419.5
86	281.0
87	220.0
88	159.0
89	101.0
90	43.0
91	33.5
92	24.0
93	17.0
94	10.0
95	6.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2061035.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.731965327197415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8130285032721	19.76550186123224
2	6.792306019371451	3.495587659643609
3	2.6911038885964067	2.0774217585974664
4	1.5884710010474072	1.6349792288884182
5	1.1455156206775865	1.473818411651934
6	0.8454012821911163	1.3052301888546023
7	0.6830111501813925	1.230265346418977
8	0.5901983058170459	1.2149569873163906
9	0.5368235084795356	1.243217151631789
>10	7.212423063945277	43.435250020013136
>50	1.0070941391341215	16.840236044881443
>100	0.08741778533405326	3.4773856697546797
>500	0.004930237651784009	0.9280153120316683
>1k	0.0020858697757547735	0.7991500489223757
>5k	0.0	0.0
>10k+	1.8962452506861576E-4	1.078984310161222
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22113	1.0729075440252107	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	2853	0.1384255968481855	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.059581715012117696	0.0
2	0.0	0.0	0.0	0.21037973639457846	0.0
3	0.0	0.0	0.0	0.42551436535527054	0.0
4	0.0	0.0	0.0	0.6156615486879166	0.0
5	0.0	0.0	0.0	0.9353552947912093	0.0
6	0.0	0.0	0.0	1.7925459781129385	0.0
7	0.0	0.0	0.0	2.335040404456984	0.0
8	0.0	0.0	0.0	3.998185377734973	0.0
9	0.0	0.0	0.0	4.963816723151233	0.0
10	0.0	0.0	0.0	6.1767510013172995	0.0
11	0.0	0.0	0.0	6.696780986252053	0.0
12	4.851931189911865E-5	0.0	0.0	7.168049062728192	0.0
13	4.851931189911865E-5	0.0	0.0	7.457515277518334	0.0
14	4.851931189911865E-5	0.0	0.0	7.60530510156305	0.0
15	4.851931189911865E-5	0.0	0.0	7.713842802281378	0.0
16	9.70386237982373E-5	0.0	0.0	7.880409600031053	0.0
17	9.70386237982373E-5	0.0	0.0	8.027131999213987	0.0
18	9.70386237982373E-5	0.0	0.0	8.310387742081042	0.0
19	9.70386237982373E-5	0.0	0.0	8.421642524265721	0.0
20	9.70386237982373E-5	0.0	0.0	8.580349193487738	0.0
21	9.70386237982373E-5	0.0	0.0	8.71955109932631	0.0
22	9.70386237982373E-5	0.0	0.0	8.856327039569925	0.0
23	9.70386237982373E-5	0.0	0.0	9.02003119791755	0.0
24	9.70386237982373E-5	0.0	0.0	9.15113037866897	0.0
25	9.70386237982373E-5	0.0	0.0	9.272622735664363	0.0
26	9.70386237982373E-5	0.0	0.0	9.384362710968032	0.0
27	9.70386237982373E-5	0.0	0.0	9.536179637900375	0.0
28	9.70386237982373E-5	0.0	0.0	9.654712316869922	0.0
29	9.70386237982373E-5	0.0	0.0	9.77649578973671	0.0
30	9.70386237982373E-5	0.0	0.0	9.981198766639091	0.0
31	9.70386237982373E-5	0.0	0.0	10.119333247615883	0.0
32	9.70386237982373E-5	0.0	0.0	10.280465882432855	0.0
33	9.70386237982373E-5	0.0	0.0	10.419279633776235	0.0
34	9.70386237982373E-5	0.0	0.0	10.552804780122608	0.0
35	9.70386237982373E-5	0.0	0.0	10.702632415267086	0.0
36	1.4555793569735593E-4	0.0	0.0	10.833343441523313	0.0
37	1.4555793569735593E-4	0.0	0.0	10.983656269786781	0.0
38	1.4555793569735593E-4	0.0	0.0	11.14289665143969	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12465	0.0	42.25271	1
TCACGAC	475	0.0	41.684208	25
TAGCCGT	325	0.0	41.29231	44
CGACGGT	495	0.0	40.88889	28
CGGTCTA	500	0.0	40.48	31
TCGTTAG	60	3.6379788E-12	40.333332	1
TACCCGT	40	4.1266685E-7	38.5	36
CATATGC	1935	0.0	38.20155	33
CACGACG	525	0.0	38.133335	26
TACGGGA	435	0.0	37.931038	4
CGACGTT	335	0.0	37.432835	27
TCGACGT	335	0.0	37.432835	26
GTTTTTT	14590	0.0	37.244686	2
TTGATCC	2020	0.0	37.138615	17
CTCACGA	535	0.0	37.00935	24
TCTCACG	535	0.0	37.00935	23
GTAGCAT	2020	0.0	36.811882	29
GTTGATC	2055	0.0	36.720196	16
GGTACCT	2135	0.0	36.683838	8
CGCGTAG	30	1.3014534E-4	36.666664	1
>>END_MODULE