FastQCFastQC Report
Thu 26 May 2016
SRR1547218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547218_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences131423
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16301.2402699679660334No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT1420.10804805855900412No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT1420.10804805855900412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG207.831021E-444.05
ACCGGCC254.4212826E-544.021
CATATGC502.7284841E-1144.033
GGGTCTA207.831021E-444.07
GCGCGAC408.216375E-944.09
CGACCTC408.216375E-944.012
AGGGTGC254.4212826E-544.06
AGGGTCT351.4330362E-744.06
GTAGGGT408.216375E-944.04
TACGGGC207.831021E-444.04
GTTGTAG254.4212826E-544.01
CTACGGG207.831021E-444.03
AACGCCA254.4212826E-544.015
TAGGGTC254.4212826E-544.05
CGGGATC207.831021E-444.06
TTAGTAG254.4212826E-544.01
GGCGCGA408.216375E-944.08
ACCCGCT351.4330362E-744.034
GACGAGG254.4212826E-544.02
AGGGCGC408.216375E-944.06
Produced by FastQC (version 0.11.5)