##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547218_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	131423
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.371243998386888	31.0	31.0	33.0	30.0	34.0
2	31.767803200353057	31.0	31.0	34.0	30.0	34.0
3	31.864521430799783	31.0	31.0	34.0	30.0	34.0
4	35.58528568058863	37.0	35.0	37.0	33.0	37.0
5	35.4681372362524	37.0	35.0	37.0	33.0	37.0
6	35.49328504143111	37.0	35.0	37.0	33.0	37.0
7	35.99532806282006	37.0	35.0	37.0	35.0	37.0
8	35.99788469293807	37.0	35.0	37.0	35.0	37.0
9	37.71478356147706	39.0	37.0	39.0	35.0	39.0
10	37.039954954612206	39.0	37.0	39.0	33.0	39.0
11	36.821256553266934	39.0	37.0	39.0	32.0	39.0
12	36.493452439831685	39.0	35.0	39.0	32.0	39.0
13	36.4048530318133	39.0	35.0	39.0	32.0	39.0
14	37.51577729925508	40.0	36.0	41.0	32.0	41.0
15	37.658248556188795	40.0	36.0	41.0	32.0	41.0
16	37.679873385937015	40.0	36.0	41.0	33.0	41.0
17	37.57343843923819	40.0	36.0	41.0	32.0	41.0
18	37.42767247742024	39.0	36.0	41.0	32.0	41.0
19	37.31884829900398	39.0	36.0	41.0	32.0	41.0
20	37.071075839084486	39.0	35.0	41.0	32.0	41.0
21	36.9745782701658	38.0	35.0	41.0	32.0	41.0
22	36.88147432336805	38.0	35.0	41.0	32.0	41.0
23	36.789131278391146	38.0	35.0	41.0	31.0	41.0
24	36.70341568827374	38.0	35.0	41.0	31.0	41.0
25	36.53446504797486	38.0	35.0	40.0	31.0	41.0
26	36.40560632461594	38.0	35.0	40.0	31.0	41.0
27	36.29929312220844	38.0	35.0	40.0	31.0	41.0
28	36.330048773806716	38.0	35.0	40.0	31.0	41.0
29	36.255860846275006	38.0	35.0	40.0	30.0	41.0
30	36.15139663529215	38.0	35.0	40.0	30.0	41.0
31	35.97736317083007	38.0	35.0	40.0	30.0	41.0
32	35.57647443750333	38.0	35.0	40.0	27.0	41.0
33	35.33686645412143	38.0	34.0	40.0	25.0	41.0
34	35.04297573484094	38.0	34.0	40.0	24.0	41.0
35	34.759912648470966	38.0	34.0	40.0	23.0	41.0
36	34.58606180044589	38.0	34.0	40.0	22.0	41.0
37	34.51977964283269	38.0	34.0	40.0	22.0	41.0
38	34.42888992033358	38.0	34.0	40.0	21.0	41.0
39	34.33132708886573	38.0	34.0	40.0	21.0	41.0
40	34.21529717020613	37.0	33.0	40.0	20.0	41.0
41	34.1861241943952	37.0	33.0	40.0	20.0	41.0
42	33.988061450431054	37.0	33.0	40.0	18.0	41.0
43	33.99247468099191	37.0	33.0	40.0	19.0	41.0
44	33.74133903502431	37.0	33.0	40.0	18.0	41.0
45	33.667272851783935	37.0	33.0	40.0	18.0	41.0
46	33.65703872229366	37.0	33.0	40.0	18.0	41.0
47	33.57078289188346	36.0	33.0	40.0	18.0	41.0
48	33.49593297976762	36.0	33.0	40.0	18.0	41.0
49	33.46787092061511	36.0	33.0	40.0	18.0	41.0
50	32.945329204172786	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	3.0
16	10.0
17	23.0
18	61.0
19	128.0
20	247.0
21	426.0
22	582.0
23	819.0
24	1219.0
25	1942.0
26	2852.0
27	3345.0
28	3297.0
29	3201.0
30	3446.0
31	4021.0
32	4696.0
33	6009.0
34	9091.0
35	13321.0
36	10113.0
37	12534.0
38	18427.0
39	31607.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.331829284067474	23.020323687634583	26.872008704716833	19.77583832358111
2	35.850650190605904	24.000365232874003	27.31485356444458	12.834131012075511
3	26.517428456206293	24.380055241472192	36.132183864315984	12.970332438005524
4	23.6024135805757	26.7434162969952	35.94271931092731	13.711450811501791
5	21.30601188528644	30.270196236579594	34.938328907421074	13.485462970712888
6	18.569808937552786	39.181878362234926	31.867329158518675	10.380983541693615
7	82.1378297558266	3.438515328367181	12.513030443681853	1.910624472124362
8	82.3775138293906	2.8655562572761237	12.077033700341646	2.6798962129916375
9	76.99869885788637	5.063801617677271	14.41756770123951	3.5199318231968526
10	37.08102843490104	28.851875242537457	20.79772946896662	13.26936685359488
11	30.21160679637506	24.304725961209225	30.288457880279708	15.195209362136003
12	27.485295572312307	21.242096132336044	33.042161569892635	18.230446725459014
13	21.68722369752631	26.725154653295085	34.21090676669989	17.376714882478712
14	18.16120465976275	29.249065993014923	34.30982400340884	18.27990534381349
15	17.471066708262633	23.513388067537647	41.52241236313278	17.493132861066936
16	19.530067035450415	21.94060400386538	39.916148619343645	18.613180341340556
17	19.299513783736487	22.162787335550092	34.33341195985482	24.2042869208586
18	21.76635748689347	23.690678191792912	34.39352320370103	20.149441117612593
19	24.24309291372134	26.611780281990217	30.254217298341995	18.89090950594645
20	25.85011755933132	24.331357524938557	29.814416045897595	20.00410886983253
21	23.129893549835266	26.99679660333427	31.0440333883719	18.829276458458565
22	22.12930765543322	23.651111297109335	31.193170145256154	23.026410902201288
23	20.88066776743797	27.423662524824422	29.901158853473138	21.794510854264473
24	21.293837456153035	24.545931838414887	34.496244949514164	19.663985755917913
25	21.015347389726305	24.991059403605153	32.95085335139207	21.042739855276473
26	18.87340876406717	28.22032673124187	31.946462947885834	20.959801556805125
27	20.526087518927433	25.248244219048416	31.98298623528606	22.242682026738088
28	19.91203974951112	26.573735190948312	34.680383190156974	18.833841869383594
29	20.470541686006253	23.931884068998578	34.10590231542424	21.491671929570927
30	21.229160801381795	24.767354268278762	33.17684119218097	20.826643738158467
31	24.178416258950108	24.36635900869711	29.24145697480654	22.213767757546243
32	24.855618879495978	24.789420421083065	29.471249324699635	20.88371137472132
33	23.180113070010577	24.95757972348828	28.06053734886588	23.801769857635268
34	20.29477336539266	26.61254118381105	30.21312860001674	22.879556850779544
35	20.429452987681	25.580758314754647	30.129429399724554	23.8603592978398
36	24.191351589904357	27.124628109235065	26.845377140987498	21.83864315987308
37	22.766943381295512	27.317136269907095	29.022317250405184	20.893603098392212
38	22.849120777946023	28.673063314640512	26.89255305387946	21.585262853534008
39	23.727962381013977	26.90853199211706	27.084300312730647	22.279205314138316
40	24.357228186847053	25.30759456107379	28.401421364601326	21.933755887477837
41	21.356992307282592	25.127260829535164	28.839700813404047	24.676046049778197
42	23.18163487365225	26.668087016732233	26.518189358027133	23.63208875158838
43	22.3408383616262	25.879031828523164	28.046080214269953	23.734049595580682
44	22.738029112103668	27.995099792273802	27.69149996575942	21.575371129863115
45	21.140135288343746	28.030862177853194	28.064341857970064	22.764660675833
46	23.387078365278526	27.973794541290335	27.085822116372327	21.55330497705881
47	22.679439671899136	25.254331433615118	28.91655189730869	23.149676997177053
48	23.482951994704123	24.10689148779133	29.079384886968036	23.33077163053651
49	22.920645549104798	23.97373366914467	28.518600245010383	24.587020536740145
50	20.602177701011236	25.835660424735394	29.935399435410847	23.626762438842515
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	140.0
1	166.5
2	193.0
3	1463.0
4	2733.0
5	1941.5
6	1150.0
7	1144.5
8	1139.0
9	1195.5
10	1252.0
11	1271.5
12	1291.0
13	1280.5
14	1270.0
15	1175.5
16	1081.0
17	1064.5
18	1048.0
19	944.5
20	841.0
21	836.0
22	831.0
23	816.0
24	801.0
25	840.0
26	879.0
27	993.0
28	1107.0
29	1333.5
30	1560.0
31	1743.5
32	1927.0
33	2254.5
34	2582.0
35	3079.5
36	3577.0
37	3938.5
38	4300.0
39	4837.5
40	5375.0
41	6187.0
42	6999.0
43	7723.5
44	8448.0
45	8920.0
46	9392.0
47	9689.5
48	9987.0
49	10679.0
50	11371.0
51	10988.0
52	10605.0
53	9428.5
54	8252.0
55	7270.0
56	6288.0
57	5921.5
58	5555.0
59	5200.0
60	4845.0
61	4514.5
62	4184.0
63	3788.0
64	3392.0
65	2779.5
66	2167.0
67	1815.5
68	1464.0
69	1306.0
70	1148.0
71	1031.5
72	915.0
73	745.5
74	576.0
75	450.5
76	325.0
77	277.5
78	230.0
79	160.5
80	91.0
81	81.5
82	72.0
83	50.0
84	28.0
85	19.0
86	10.0
87	5.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	131423.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.30474878826385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34282486147336	47.06253852065468
2	13.120665408608485	16.612008552536466
3	6.037477312883878	11.466029538208684
4	2.8919311994422876	7.3229191237454625
5	1.556546510090508	4.926839289926421
6	0.848588290443165	3.223180113070011
7	0.46275707056744836	2.050630407158564
8	0.25481688041636114	1.2904894881413451
9	0.12981237304229717	0.7395965698545917
>10	0.3389545296104427	3.2954657860496264
>50	0.010817697753524766	0.47023732527791934
>100	0.003605899251174922	0.2997953174101946
>500	0.0	0.0
>1k	0.0012019664170583074	1.2402699679660334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1630	1.2402699679660334	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	142	0.10804805855900412	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	142	0.10804805855900412	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04337140378776926	0.0
2	0.0	0.0	0.0	0.1673984005843726	0.0
3	0.0	0.0	0.0	0.41240878689422705	0.0
4	0.0	0.0	0.0	0.5904598129703325	0.0
5	0.0	0.0	0.0	0.9244957123182396	0.0
6	0.0	0.0	0.0	1.7827929662235682	0.0
7	0.0	0.0	0.0	2.313902437168532	0.0
8	0.0	0.0	0.0	3.7489632712691083	0.0
9	0.0	0.0	0.0	4.710743172808413	0.0
10	0.0	0.0	0.0	5.83992147493209	0.0
11	0.0	0.0	0.0	6.299506174718276	0.0
12	0.0	0.0	0.0	6.774308910921224	0.0
13	0.0	0.0	0.0	7.0862786574648275	0.0
14	0.0	0.0	0.0	7.260525174436743	0.0
15	0.0	0.0	0.0	7.3952047967250785	0.0
16	0.0	0.0	0.0	7.565646804592803	0.0
17	0.0	0.0	0.0	7.765763983473213	0.0
18	0.0	0.0	0.0	8.086864551866872	0.0
19	0.0	0.0	0.0	8.207847941380123	0.0
20	0.0	0.0	0.0	8.391986182022933	0.0
21	0.0	0.0	0.0	8.55405826986144	0.0
22	0.0	0.0	0.0	8.746566430533468	0.0
23	0.0	0.0	0.0	8.929182867534601	0.0
24	0.0	0.0	0.0	9.076797820777186	0.0
25	0.0	0.0	0.0	9.197020308469599	0.0
26	0.0	0.0	0.0	9.350722476278886	0.0
27	0.0	0.0	0.0	9.541708833309238	0.0
28	0.0	0.0	0.0	9.704541822968583	0.0
29	0.0	0.0	0.0	9.871179321732116	0.0
30	0.0	0.0	0.0	10.091079947954315	0.0
31	0.0	0.0	0.0	10.244782115763604	0.0
32	0.0	0.0	0.0	10.427398552764737	0.0
33	0.0	0.0	0.0	10.635124749853526	0.0
34	0.0	0.0	0.0	10.781217899454433	0.0
35	0.0	0.0	0.0	10.95470351460551	0.0
36	0.0	0.0	0.0	11.12362371883156	0.0
37	0.0	0.0	0.0	11.28721761031174	0.0
38	0.0	0.0	0.0	11.515488156563158	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTG	20	7.831021E-4	44.0	5
ACCGGCC	25	4.4212826E-5	44.0	21
CATATGC	50	2.7284841E-11	44.0	33
GGGTCTA	20	7.831021E-4	44.0	7
GCGCGAC	40	8.216375E-9	44.0	9
CGACCTC	40	8.216375E-9	44.0	12
AGGGTGC	25	4.4212826E-5	44.0	6
AGGGTCT	35	1.4330362E-7	44.0	6
GTAGGGT	40	8.216375E-9	44.0	4
TACGGGC	20	7.831021E-4	44.0	4
GTTGTAG	25	4.4212826E-5	44.0	1
CTACGGG	20	7.831021E-4	44.0	3
AACGCCA	25	4.4212826E-5	44.0	15
TAGGGTC	25	4.4212826E-5	44.0	5
CGGGATC	20	7.831021E-4	44.0	6
TTAGTAG	25	4.4212826E-5	44.0	1
GGCGCGA	40	8.216375E-9	44.0	8
ACCCGCT	35	1.4330362E-7	44.0	34
GACGAGG	25	4.4212826E-5	44.0	2
AGGGCGC	40	8.216375E-9	44.0	6
>>END_MODULE