FastQCFastQC Report
Thu 26 May 2016
SRR1547217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547217_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2326718
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121720.5231403203998077No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49460.21257410653117395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27330.11746159182161311No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC207.8581466E-444.040
CTATCGT254.4442502E-544.013
TAGACGT254.4442502E-544.039
GCACGAC207.8581466E-444.026
TACGACT207.8581466E-444.036
TCTAACG302.528841E-644.01
CGTACCA254.4442502E-544.025
CGCACGA302.528841E-644.039
TCGAACA207.8581466E-444.040
CGGTCTA4050.042.3703731
CGTTTTT79200.041.9444431
CACGACG4150.041.87951726
CTCACGA4300.040.41860224
TACGGGT2250.040.088894
CATATGC12700.039.84251833
ACGTTAG1000.039.6000021
ACGGGTA3450.039.5362325
ACGGTCT4400.039.00000430
TAGCATA13000.038.92307730
GTTGATC13350.038.7265916
Produced by FastQC (version 0.11.5)