##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547217_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2326718
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.361352772445994	31.0	31.0	33.0	30.0	34.0
2	31.753108455773326	31.0	31.0	34.0	30.0	34.0
3	31.881211646619832	31.0	31.0	34.0	30.0	34.0
4	35.55404307698655	37.0	35.0	37.0	33.0	37.0
5	35.430275177309845	37.0	35.0	37.0	33.0	37.0
6	35.46440909469906	37.0	35.0	37.0	33.0	37.0
7	35.957826002119724	37.0	35.0	37.0	35.0	37.0
8	35.95802757360368	37.0	35.0	37.0	35.0	37.0
9	37.67481104285092	39.0	37.0	39.0	35.0	39.0
10	37.04843560758115	39.0	37.0	39.0	33.0	39.0
11	36.77485582696313	39.0	35.0	39.0	32.0	39.0
12	36.027474751989715	37.0	35.0	39.0	32.0	39.0
13	35.675033244252205	37.0	35.0	39.0	31.0	39.0
14	36.67581202363157	38.0	35.0	41.0	31.0	41.0
15	36.98403416314311	38.0	35.0	41.0	32.0	41.0
16	37.10894917218159	38.0	35.0	41.0	32.0	41.0
17	37.05675032384672	38.0	35.0	41.0	32.0	41.0
18	37.02785683525034	38.0	35.0	41.0	32.0	41.0
19	36.98006720195571	38.0	35.0	41.0	32.0	41.0
20	36.80742015147517	38.0	35.0	41.0	32.0	41.0
21	36.67340047225319	38.0	35.0	41.0	31.0	41.0
22	36.5849729103398	38.0	35.0	40.0	31.0	41.0
23	36.51767038377663	38.0	35.0	40.0	31.0	41.0
24	36.432703920286	38.0	35.0	40.0	31.0	41.0
25	36.24991769522563	38.0	35.0	40.0	31.0	41.0
26	36.0950510547475	37.0	35.0	40.0	31.0	41.0
27	35.996118137221615	37.0	35.0	40.0	30.0	41.0
28	36.075829559061305	37.0	35.0	40.0	30.0	41.0
29	36.01917980606159	37.0	35.0	40.0	30.0	41.0
30	35.98759755157264	37.0	35.0	40.0	30.0	41.0
31	35.864181220070506	37.0	35.0	40.0	30.0	41.0
32	35.58515041358687	37.0	34.0	40.0	30.0	41.0
33	35.53556382853444	37.0	34.0	40.0	29.0	41.0
34	35.43403583932389	37.0	34.0	40.0	29.0	41.0
35	35.30328385304966	37.0	34.0	40.0	29.0	41.0
36	35.11956541359976	37.0	34.0	40.0	27.0	41.0
37	35.071617187815626	37.0	34.0	40.0	27.0	41.0
38	35.02006216481757	37.0	34.0	40.0	27.0	41.0
39	34.93732931966831	36.0	34.0	40.0	27.0	41.0
40	34.80799907853036	36.0	34.0	40.0	26.0	41.0
41	34.8126567121585	36.0	34.0	40.0	26.0	41.0
42	34.609658325589955	36.0	34.0	40.0	25.0	41.0
43	34.6356090424366	36.0	34.0	40.0	26.0	41.0
44	34.40330843703448	35.0	34.0	40.0	25.0	41.0
45	34.3168867047919	35.0	34.0	40.0	24.0	41.0
46	34.31313850668624	35.0	34.0	40.0	25.0	41.0
47	34.199967507880196	35.0	33.0	40.0	24.0	41.0
48	34.16706021099248	35.0	33.0	40.0	24.0	41.0
49	34.18007296114097	35.0	34.0	40.0	25.0	41.0
50	33.614338738085145	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	8.0
13	10.0
14	27.0
15	40.0
16	136.0
17	434.0
18	934.0
19	1848.0
20	3405.0
21	5486.0
22	8392.0
23	12309.0
24	17682.0
25	24833.0
26	33038.0
27	40594.0
28	46141.0
29	52202.0
30	61660.0
31	75470.0
32	93516.0
33	120713.0
34	204665.0
35	351971.0
36	173708.0
37	209865.0
38	297338.0
39	490291.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.17566546526051	25.078243259389403	28.521247525484394	14.22484374986569
2	31.363448428215197	26.327814543919803	29.343134836280115	12.965602191584885
3	28.45187942844814	26.641088434438554	31.082365804536693	13.824666332576616
4	25.752798577223366	28.747317036271692	31.15500030515086	14.344884081354078
5	22.70322402628939	32.79439106930879	29.93233387114382	14.570051033258006
6	19.4438260244688	41.944146217977426	27.211677564707028	11.400350192846748
7	85.85432355790431	4.738434137699541	7.189612148958319	2.2176301554378313
8	85.75035737033883	3.9900409074069136	6.5998114081723696	3.6597903140818957
9	80.52050141014081	5.839770870384808	9.712694017925678	3.927033701548705
10	47.66310313497381	25.381631981185514	13.815296911787334	13.139967972053338
11	42.66748269450788	21.961234666169258	21.31611995953098	14.055162679791877
12	37.783177849657754	21.116310614350343	24.317128246740687	16.783383289251212
13	22.14741967011043	36.32679164385198	26.11915152588324	15.406637160154347
14	16.48300309706634	38.86534595081999	27.05978979833396	17.5918611537797
15	14.90550208491102	22.697206967066915	45.60152970837033	16.795761239651732
16	16.22744999608891	18.99727427217222	43.52796514231635	21.247310589422526
17	16.589848877259726	20.528314991331136	28.864047985187717	34.01778814622142
18	22.02256569124406	22.897317165208676	32.47557288850647	22.604544255040793
19	27.52465060226465	26.71307825013603	24.472282416691666	21.289988730907655
20	29.928809593599226	24.202460289558083	23.44160315087604	22.427126965966654
21	22.9751091451564	30.25226950580173	25.171851509293347	21.60076983974852
22	22.255125030192744	25.720650289377573	23.41199062370257	28.612234056727115
23	19.708060882324375	31.40934999428379	23.02565244262519	25.85693668076664
24	20.89621518379107	24.687865052834077	34.58691599067872	19.829003772696133
25	19.42212163227344	25.88366961531221	31.51954813604399	23.174660616370353
26	17.91235551536542	32.48795083890699	26.732074965681274	22.867618680046313
27	20.465995449384067	30.457193351321475	26.54808189045686	22.5287293088376
28	18.24432526846829	27.83207934953871	34.37588912794761	19.547706254045398
29	17.868602899019134	24.89850510461517	33.04083262346361	24.19205937290209
30	20.716520008011287	29.11405679588158	28.44612024319234	21.723302952914793
31	27.94562985286571	26.238117382510474	21.71909101145906	24.09716175316476
32	27.861219107773266	28.028063564213628	22.631492084558595	21.479225243454515
33	25.61393344616752	26.70847949773028	23.232854174850583	24.444732881251614
34	19.312482217441048	27.12902895838688	26.108406777271675	27.4500820469004
35	20.394865213575518	24.265854306366307	29.7317079250687	25.60757255498947
36	27.512315630858573	23.933239868346746	25.622572224051215	22.931872276743466
37	22.48764139014698	27.943137071187827	28.491506061327588	21.077715477337605
38	21.63304706457766	28.2435172633727	25.918439621819232	24.20499605023041
39	21.228872600805083	28.223145220005176	27.368851747396977	23.179130431792768
40	23.97772312759862	24.093852370592398	27.76026144981902	24.16816305198997
41	18.844097135965768	22.78965478412081	28.63741974747262	29.728828332440806
42	23.296162233669914	25.79870014329197	24.758092729759255	26.14704489327886
43	22.86125778886827	24.95188501571742	26.838276060957966	25.348581134456342
44	21.792671049951046	28.529542471412523	26.993645125881176	22.684141352755255
45	19.130208301994482	31.351973036698045	25.198627422833365	24.319191238474108
46	23.75062212094461	28.924562409368043	26.033021621012946	21.2917938486744
47	23.258598592523892	25.19793975892222	26.95401849300173	24.589443155552154
48	23.40524292157451	22.405078741815725	28.815438742469006	25.374239594140757
49	21.982939058364614	21.855334423853687	29.75607701491973	26.405649502861973
50	20.455465595744734	27.420598456710266	28.172086174603024	23.951849772941973
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3303.0
1	3269.5
2	3236.0
3	11155.0
4	19074.0
5	13712.5
6	8351.0
7	8893.0
8	9435.0
9	10226.0
10	11017.0
11	11198.0
12	11379.0
13	11205.5
14	11032.0
15	10567.5
16	10103.0
17	9544.0
18	8985.0
19	8939.5
20	8894.0
21	8350.0
22	7806.0
23	8143.0
24	8480.0
25	9155.5
26	9831.0
27	11799.0
28	13767.0
29	16751.5
30	19736.0
31	22763.0
32	25790.0
33	29911.5
34	34033.0
35	39959.5
36	45886.0
37	49472.0
38	53058.0
39	70758.5
40	88459.0
41	121109.5
42	153760.0
43	181596.5
44	209433.0
45	222056.0
46	234679.0
47	234831.0
48	234983.0
49	231486.0
50	227989.0
51	201835.5
52	175682.0
53	151852.0
54	128022.0
55	117333.0
56	106644.0
57	101460.5
58	96277.0
59	92009.5
60	87742.0
61	81569.5
62	75397.0
63	67091.0
64	58785.0
65	48457.0
66	38129.0
67	32944.0
68	27759.0
69	24436.0
70	21113.0
71	18470.0
72	15827.0
73	12748.5
74	9670.0
75	7810.0
76	5950.0
77	4773.5
78	3597.0
79	2729.5
80	1862.0
81	1371.5
82	881.0
83	679.5
84	478.0
85	356.0
86	234.0
87	175.5
88	117.0
89	66.5
90	16.0
91	18.5
92	21.0
93	13.5
94	6.0
95	7.5
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2326718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.130490593704437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.26148158744729	17.540954727140797
2	8.106168805379104	3.5878698499684516
3	2.7295663949029945	1.8121993028187733
4	1.39851813788788	1.2379956998261106
5	0.9066619962157957	1.0032437389461477
6	0.5931538185140154	0.7876071000746572
7	0.453119610356468	0.7019431492371784
8	0.36794744896413656	0.6514286046622693
9	0.3041368794048638	0.6057628513981163
>10	3.4974090018565054	18.159318288503094
>50	1.487540811925045	23.905179501168934
>100	0.8868691057622639	27.80818744403008
>500	0.00469035876863202	0.692345603870768
>1k	0.0025406109996756777	0.9795254395592925
>5k	0.0	0.0
>10k+	1.954316153596675E-4	0.526438698795289
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12172	0.5231403203998077	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4946	0.21257410653117395	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2733	0.11746159182161311	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.086688631798095	0.0
2	0.0	0.0	0.0	0.3331301859529174	0.0
3	0.0	0.0	0.0	0.5435123637673324	0.0
4	0.0	0.0	0.0	0.8399814674575947	0.0
5	0.0	0.0	0.0	1.3925194200586406	0.0
6	0.0	0.0	0.0	2.502494930627605	0.0
7	0.0	0.0	0.0	3.1981099557402315	0.0
8	0.0	0.0	0.0	5.157608270533859	0.0
9	0.0	0.0	0.0	6.311981082365804	0.0
10	0.0	0.0	0.0	7.854153361086302	0.0
11	0.0	0.0	0.0	8.63203018156906	0.0
12	0.0	0.0	0.0	9.44579446241444	0.0
13	0.0	0.0	0.0	9.842662497131153	0.0
14	0.0	0.0	0.0	10.011741861282717	0.0
15	0.0	0.0	0.0	10.164747081511383	0.0
16	0.0	0.0	0.0	10.468092824313045	0.0
17	0.0	0.0	0.0	10.769461533370182	0.0
18	0.0	0.0	0.0	11.333259982516145	0.0
19	0.0	0.0	0.0	11.516264540868296	0.0
20	0.0	0.0	0.0	11.759998418373005	0.0
21	0.0	0.0	0.0	11.981211302787875	0.0
22	0.0	0.0	0.0	12.201306733347144	0.0
23	0.0	0.0	0.0	12.466873940030549	0.0
24	0.0	0.0	0.0	12.675880790022685	0.0
25	0.0	0.0	0.0	12.829100905223582	0.0
26	0.0	0.0	0.0	12.987607436741367	0.0
27	4.2978994446254334E-5	0.0	0.0	13.159523414526385	0.0
28	4.2978994446254334E-5	4.2978994446254334E-5	0.0	13.319405273866451	0.0
29	4.2978994446254334E-5	4.2978994446254334E-5	0.0	13.488398680029123	0.0
30	4.2978994446254334E-5	4.2978994446254334E-5	0.0	13.751902894979107	0.0
31	4.2978994446254334E-5	4.2978994446254334E-5	0.0	13.941956008420444	0.0
32	4.2978994446254334E-5	4.2978994446254334E-5	0.0	14.109574086760837	0.0
33	4.2978994446254334E-5	4.2978994446254334E-5	0.0	14.280372610690252	0.0
34	8.595798889250867E-5	4.2978994446254334E-5	0.0	14.458047773731067	0.0
35	8.595798889250867E-5	4.2978994446254334E-5	0.0	14.66477673701755	0.0
36	8.595798889250867E-5	4.2978994446254334E-5	0.0	14.83574717692475	0.0
37	8.595798889250867E-5	4.2978994446254334E-5	0.0	15.01037083135988	0.0
38	8.595798889250867E-5	4.2978994446254334E-5	0.0	15.185510233728367	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.8581466E-4	44.0	40
CTATCGT	25	4.4442502E-5	44.0	13
TAGACGT	25	4.4442502E-5	44.0	39
GCACGAC	20	7.8581466E-4	44.0	26
TACGACT	20	7.8581466E-4	44.0	36
TCTAACG	30	2.528841E-6	44.0	1
CGTACCA	25	4.4442502E-5	44.0	25
CGCACGA	30	2.528841E-6	44.0	39
TCGAACA	20	7.8581466E-4	44.0	40
CGGTCTA	405	0.0	42.37037	31
CGTTTTT	7920	0.0	41.944443	1
CACGACG	415	0.0	41.879517	26
CTCACGA	430	0.0	40.418602	24
TACGGGT	225	0.0	40.08889	4
CATATGC	1270	0.0	39.842518	33
ACGTTAG	100	0.0	39.600002	1
ACGGGTA	345	0.0	39.536232	5
ACGGTCT	440	0.0	39.000004	30
TAGCATA	1300	0.0	38.923077	30
GTTGATC	1335	0.0	38.72659	16
>>END_MODULE