FastQCFastQC Report
Thu 26 May 2016
SRR1547216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547216_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2471603
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102730.415641185093237No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42170.1706180159192233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC254.4443328E-544.015
CGTTTTT76450.040.7769781
ACGGGAT6750.040.740745
TACGGGA9450.039.576724
TATACGG2900.038.6896552
CGTTAGG3500.038.3428572
CGGTCTA4650.037.8494631
ACGTACA357.2936073E-637.71428732
TCACGAC4450.037.57303225
TTATACG1000.037.3999981
CACGACG4550.037.23076626
TAACGCC651.0913936E-1137.23076612
TAGGGTA13350.037.0786555
TATAGCG2150.036.8372081
ATTAGCG2400.036.6666681
TAAGGGA26300.036.136884
TATGGGC8650.036.115614
CGACGGT4700.036.04255728
AGGGCGA29750.035.9394956
GGGCGAT54800.035.8503657
Produced by FastQC (version 0.11.5)