##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547216_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2471603
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.557486780846276	31.0	30.0	31.0	27.0	33.0
2	30.88334777065734	31.0	31.0	33.0	28.0	34.0
3	31.06786729098484	31.0	31.0	34.0	28.0	34.0
4	34.88904528761294	35.0	35.0	37.0	32.0	37.0
5	34.67107500678709	35.0	35.0	37.0	32.0	37.0
6	34.70693351642638	35.0	35.0	37.0	31.0	37.0
7	35.36197560854231	35.0	35.0	37.0	33.0	37.0
8	34.34282771140835	35.0	35.0	37.0	32.0	37.0
9	36.44379740597499	39.0	37.0	39.0	32.0	39.0
10	35.82820177836004	37.0	35.0	39.0	30.0	39.0
11	35.569173123677224	37.0	35.0	39.0	30.0	39.0
12	34.984714373627156	37.0	34.0	39.0	30.0	39.0
13	34.66373118983915	37.0	33.0	39.0	27.0	39.0
14	35.53119210488092	38.0	33.0	40.0	27.0	41.0
15	35.84084539466897	38.0	33.0	40.0	29.0	41.0
16	35.99355802691613	38.0	34.0	40.0	30.0	41.0
17	35.91454493298479	38.0	34.0	40.0	29.0	41.0
18	35.8501466457194	37.0	34.0	40.0	29.0	41.0
19	35.835866035119714	37.0	34.0	40.0	29.0	41.0
20	35.68912725870619	37.0	34.0	40.0	29.0	41.0
21	35.486113263335575	37.0	34.0	40.0	29.0	41.0
22	35.434845725628264	37.0	33.0	40.0	28.0	41.0
23	35.344699371217786	37.0	34.0	40.0	28.0	41.0
24	35.25924915935124	37.0	34.0	40.0	27.0	41.0
25	35.04999144280048	36.0	33.0	40.0	27.0	41.0
26	34.864786537320114	36.0	33.0	40.0	27.0	41.0
27	34.62343992947088	36.0	33.0	40.0	25.0	41.0
28	34.65552032425919	36.0	33.0	40.0	25.0	41.0
29	34.52257745277053	36.0	33.0	40.0	25.0	41.0
30	34.55724078664737	36.0	33.0	40.0	25.0	41.0
31	34.51351895915323	36.0	33.0	40.0	25.0	41.0
32	34.28712701837633	36.0	33.0	40.0	24.0	41.0
33	34.164113330498466	36.0	33.0	40.0	24.0	41.0
34	34.06637230979247	36.0	33.0	40.0	24.0	41.0
35	33.871877886537604	36.0	32.0	40.0	23.0	41.0
36	33.780441276370034	36.0	32.0	40.0	23.0	41.0
37	33.76620557589548	36.0	32.0	40.0	23.0	41.0
38	33.791760246285506	36.0	32.0	40.0	23.0	41.0
39	33.70013347612865	36.0	32.0	40.0	23.0	41.0
40	33.58740703907545	35.0	32.0	40.0	23.0	41.0
41	33.611228421392916	35.0	32.0	40.0	23.0	41.0
42	33.46645193423054	35.0	32.0	39.0	23.0	41.0
43	33.50777774585967	35.0	32.0	39.0	23.0	41.0
44	33.1991185477603	35.0	31.0	39.0	22.0	41.0
45	33.09210540689585	35.0	31.0	39.0	22.0	41.0
46	33.04488868155606	35.0	31.0	39.0	22.0	41.0
47	32.952196610863474	35.0	31.0	39.0	22.0	41.0
48	32.96014691679853	35.0	31.0	39.0	22.0	40.0
49	32.96285042541217	35.0	31.0	39.0	22.0	40.0
50	32.38290898659696	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	10.0
13	10.0
14	44.0
15	143.0
16	462.0
17	1063.0
18	2257.0
19	4319.0
20	7087.0
21	10972.0
22	16047.0
23	22310.0
24	30242.0
25	39773.0
26	51036.0
27	62423.0
28	72836.0
29	84813.0
30	100826.0
31	119924.0
32	142733.0
33	173596.0
34	238516.0
35	264460.0
36	219472.0
37	251887.0
38	288397.0
39	265942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.17183099389344	23.886239011685937	28.079833209459608	13.862096784961015
2	30.511453497993003	26.548559780838588	28.98483292017367	13.955153800994738
3	28.856171480613995	26.226420667073153	30.50497996644283	14.412427885870022
4	25.799450801767108	29.180900006999504	29.636029734548792	15.383619456684588
5	22.645829447528588	33.32703512659598	29.231231714802092	14.795903711073342
6	21.31365757364755	42.5118435282689	25.87644536764197	10.29805353044158
7	89.37462853055284	3.73348794284519	5.611216688116983	1.2806668384849833
8	87.5445611613192	5.59681308041785	5.210505085161331	1.6481206731016267
9	85.06778799022335	4.80376500594958	7.261036663250531	2.8674103405765408
10	44.460012388721005	29.59921152385719	13.545986147451675	12.394789939970133
11	38.688009360726625	24.49661211772279	21.481322040797004	15.334056480753583
12	34.57290673299879	22.04079700502063	25.843349437591716	17.54294682438887
13	24.31167141324881	31.604104704517678	26.554669176239067	17.52955470599445
14	19.764622392835744	34.17850682330455	28.597837112189943	17.45903367166976
15	18.977481415907004	23.246856392389876	39.47098300171994	18.30467918998318
16	21.481038823791685	20.754627664717997	37.73057404445617	20.033759467034148
17	21.41755775502781	20.446528022501994	28.537714187917718	29.598200034552473
18	23.401695175155556	23.131061096786173	31.614543274142328	21.85270045391594
19	29.283950537363808	24.069318575839244	25.557300262218487	21.089430624578462
20	30.438990404203263	24.265830717959155	24.59201578894345	20.70316308889413
21	25.532417625322513	26.706271193229657	27.358560416053873	20.402750765393957
22	25.827974800160057	23.331416898264003	26.178314235740935	24.662294065835006
23	22.79338550730032	28.672849159027564	25.257535291873328	23.276230041798783
24	22.69373358099986	24.14465429925437	33.302516625849705	19.859095493896067
25	22.465784351289425	24.84881269362434	30.87368804779732	21.811714907288913
26	20.990790187582714	29.867498947039632	26.17398506151676	22.967725803860894
27	21.16962149665622	28.778731859445067	27.93417874958074	22.11746789431798
28	19.91412051207253	27.345491974236964	32.57525581576006	20.165131697930452
29	20.73561166578937	25.31717270127929	31.73300890151048	22.214206731420862
30	22.42309950263048	26.932399742191603	29.588813413804726	21.05568734137319
31	28.358235525689196	25.18899677658588	25.22180948963082	21.2309582080941
32	28.83282630746119	25.588373213659317	25.49046104896296	20.088339429916534
33	27.911278631722002	25.2987231363613	25.27910024384984	21.510897988066855
34	22.27420827697652	26.62239850008274	27.888944947873913	23.21444827506683
35	22.666827965494456	25.960965413943903	28.973746997394002	22.398459623167636
36	28.59512632085331	26.13639811895357	24.500091640931007	20.768383919262114
37	22.962546978620757	29.85398544992865	26.580522842867566	20.60294472858303
38	24.02598637402528	30.52185160804547	23.874343897462495	21.57781812046676
39	22.746735620566895	29.376198361953758	25.144410328034073	22.732655689445274
40	24.90586069041023	24.975612992863336	26.442434322987957	23.676091993738478
41	20.879243147058812	24.40630635259789	26.794756277606073	27.919694222737228
42	23.079394223101364	26.159500534673246	25.07579089360225	25.68531434862314
43	22.843595836386346	25.46582116950012	26.740499991301192	24.950083002812345
44	22.472905235994617	27.027601115551324	28.142788303785032	22.356705344669024
45	20.934025407802142	30.435632259711614	25.440412558165693	23.189929774320554
46	23.185115085230112	28.653145347371726	26.3448862944413	21.816853272956862
47	22.966269259262106	26.738193795686442	26.99567851309454	23.299858431956913
48	23.4079259492726	25.47031218201305	28.636637841918787	22.485124026795567
49	23.044396693158244	24.549047723279184	29.244947509774022	23.16160807378855
50	21.550426990095094	29.4900920576646	26.232044547607362	22.727436404632943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1763.0
1	2562.5
2	3362.0
3	10889.0
4	18416.0
5	13540.5
6	8665.0
7	9013.5
8	9362.0
9	9630.5
10	9899.0
11	10116.5
12	10334.0
13	10261.0
14	10188.0
15	9914.5
16	9641.0
17	9782.5
18	9924.0
19	9947.0
20	9970.0
21	11005.0
22	12040.0
23	12112.5
24	12185.0
25	13686.0
26	15187.0
27	18756.5
28	22326.0
29	25852.5
30	29379.0
31	34397.0
32	39415.0
33	47994.0
34	56573.0
35	60996.0
36	65419.0
37	69460.5
38	73502.0
39	90212.0
40	106922.0
41	132166.5
42	157411.0
43	171390.0
44	185369.0
45	189683.5
46	193998.0
47	192154.0
48	190310.0
49	186701.0
50	183092.0
51	171305.0
52	159518.0
53	151681.5
54	143845.0
55	138821.5
56	133798.0
57	129087.0
58	124376.0
59	118339.0
60	112302.0
61	104628.5
62	96955.0
63	88121.0
64	79287.0
65	67474.0
66	55661.0
67	48027.0
68	40393.0
69	35049.0
70	29705.0
71	25127.0
72	20549.0
73	17271.5
74	13994.0
75	10643.5
76	7293.0
77	5760.5
78	4228.0
79	3354.5
80	2481.0
81	1879.0
82	1277.0
83	996.0
84	715.0
85	545.0
86	375.0
87	259.5
88	144.0
89	94.5
90	45.0
91	25.5
92	6.0
93	4.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2471603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.255451080579753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32294589526671	17.498113492860554
2	5.993114815124061	2.547727175463345
3	1.9294548821335502	1.2303430157812636
4	1.044625142736502	0.8881591447591746
5	0.6578584323702312	0.6991538863596167
6	0.47450273194966053	0.6051461763934474
7	0.3963477245830761	0.589718476954227
8	0.3176909881059168	0.5402132205141087
9	0.26427549685029894	0.5055565405587694
>10	3.824701109471916	20.555513514520797
>50	1.9617120582473158	30.04303020425933
>100	0.8088883721408557	23.156924503588083
>500	0.002911763264938337	0.4027030762457265
>1k	7.764702039835564E-4	0.31382782765624956
>5k	0.0	0.0
>10k+	1.941175509958891E-4	0.42386974408528155
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10273	0.415641185093237	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4217	0.1706180159192233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01836864577361332	0.0
2	0.0	0.0	0.0	0.07501204683761915	0.0
3	0.0	0.0	0.0	0.11680678490841774	0.0
4	0.0	0.0	0.0	0.204199460835741	0.0
5	0.0	0.0	0.0	0.3425711977206695	0.0
6	0.0	0.0	0.0	0.5881203413331348	0.0
7	0.0	0.0	0.0	0.7069096452787927	0.0
8	0.0	0.0	0.0	1.0366956181878724	0.0
9	0.0	0.0	0.0	1.183240188654893	0.0
10	0.0	0.0	0.0	1.4006294700241098	0.0
11	0.0	0.0	0.0	1.556762959099823	0.0
12	4.0459572188575594E-5	0.0	0.0	1.698047785182329	0.0
13	4.0459572188575594E-5	0.0	0.0	1.7651702154431759	0.0
14	4.0459572188575594E-5	0.0	0.0	1.795676732873362	0.0
15	4.0459572188575594E-5	0.0	0.0	1.8301078288058397	0.0
16	4.0459572188575594E-5	0.0	0.0	1.9018426502961843	0.0
17	4.0459572188575594E-5	0.0	0.0	1.9794036501816836	0.0
18	4.0459572188575594E-5	0.0	0.0	2.1042214303834395	0.0
19	4.0459572188575594E-5	0.0	0.0	2.151842346849393	0.0
20	4.0459572188575594E-5	0.0	0.0	2.207757475614004	0.0
21	4.0459572188575594E-5	0.0	0.0	2.277671616355863	0.0
22	4.0459572188575594E-5	0.0	0.0	2.349527816562773	0.0
23	4.0459572188575594E-5	0.0	0.0	2.439226688104845	0.0
24	4.0459572188575594E-5	0.0	0.0	2.5095454245685898	0.0
25	4.0459572188575594E-5	0.0	0.0	2.5666743404988583	0.0
26	4.0459572188575594E-5	0.0	0.0	2.6222657926859614	0.0
27	4.0459572188575594E-5	0.0	0.0	2.682914691396636	0.0
28	4.0459572188575594E-5	0.0	0.0	2.744858296417345	0.0
29	4.0459572188575594E-5	0.0	0.0	2.812668539405398	0.0
30	4.0459572188575594E-5	0.0	0.0	2.903702576829693	0.0
31	4.0459572188575594E-5	0.0	0.0	2.979887951260781	0.0
32	4.0459572188575594E-5	0.0	0.0	3.059107793606012	0.0
33	4.0459572188575594E-5	0.0	0.0	3.136223738197437	0.0
34	4.0459572188575594E-5	0.0	0.0	3.218397129312434	0.0
35	4.0459572188575594E-5	0.0	0.0	3.3138008005330954	0.0
36	4.0459572188575594E-5	0.0	0.0	3.395812353359338	0.0
37	4.0459572188575594E-5	0.0	0.0	3.486563173778313	0.0
38	4.0459572188575594E-5	0.0	0.0	3.5815622492770887	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTATC	25	4.4443328E-5	44.0	15
CGTTTTT	7645	0.0	40.776978	1
ACGGGAT	675	0.0	40.74074	5
TACGGGA	945	0.0	39.57672	4
TATACGG	290	0.0	38.689655	2
CGTTAGG	350	0.0	38.342857	2
CGGTCTA	465	0.0	37.84946	31
ACGTACA	35	7.2936073E-6	37.714287	32
TCACGAC	445	0.0	37.573032	25
TTATACG	100	0.0	37.399998	1
CACGACG	455	0.0	37.230766	26
TAACGCC	65	1.0913936E-11	37.230766	12
TAGGGTA	1335	0.0	37.078655	5
TATAGCG	215	0.0	36.837208	1
ATTAGCG	240	0.0	36.666668	1
TAAGGGA	2630	0.0	36.13688	4
TATGGGC	865	0.0	36.11561	4
CGACGGT	470	0.0	36.042557	28
AGGGCGA	2975	0.0	35.939495	6
GGGCGAT	5480	0.0	35.850365	7
>>END_MODULE