FastQCFastQC Report
Thu 26 May 2016
SRR1547215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547215_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3115181
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392461.2598304881803015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT339850.043.0160331
GCGATAC1700.038.823539
CGGTCTA2750.038.431
GTTTTTT376850.038.232192
CGTTCGA750.038.1333314
CGACGGT2800.037.71428728
CGCGTAA651.0913936E-1137.23076631
CACGACG3100.036.90322526
AGGGCGA30950.036.820686
GGGCGAT61000.036.4622967
CGTTAGG4300.036.325582
TACGGGA11900.036.23534
TAGGGAC31800.036.1132055
CGTAAGG4650.035.956992
ACGGGAT14700.035.9183655
AGGGATT57100.035.8318756
TAAGGGA39600.035.722224
TAGGGAT48250.035.336795
AAGGGAT53300.035.043155
ATTGGGA31800.034.9371074
Produced by FastQC (version 0.11.5)