##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547215_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3115181 Sequences flagged as poor quality 0 Sequence length 50 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.578861067783862 31.0 30.0 31.0 27.0 34.0 2 30.924859261789283 31.0 31.0 33.0 28.0 34.0 3 31.005442701403226 31.0 31.0 34.0 28.0 34.0 4 34.85615956183605 35.0 35.0 37.0 32.0 37.0 5 34.677744567651125 35.0 35.0 37.0 32.0 37.0 6 34.71574974295233 35.0 35.0 37.0 31.0 37.0 7 35.42010143230843 35.0 35.0 37.0 33.0 37.0 8 34.38907402170211 36.0 35.0 37.0 32.0 37.0 9 36.48756781708671 39.0 37.0 39.0 32.0 39.0 10 35.87575232386176 38.0 35.0 39.0 30.0 39.0 11 35.66902019497422 37.0 35.0 39.0 30.0 39.0 12 35.46712149310104 37.0 35.0 39.0 30.0 39.0 13 35.40866774675372 37.0 35.0 39.0 30.0 39.0 14 36.33967207683919 38.0 35.0 40.0 30.0 41.0 15 36.45752686601517 38.0 35.0 40.0 30.0 41.0 16 36.48041478167721 38.0 35.0 40.0 30.0 41.0 17 36.37515861839167 38.0 34.0 40.0 30.0 41.0 18 36.19935214037322 38.0 34.0 40.0 30.0 41.0 19 36.077773972042074 37.0 34.0 40.0 30.0 41.0 20 35.84116653253856 37.0 34.0 40.0 29.0 41.0 21 35.67787874926048 37.0 34.0 40.0 29.0 41.0 22 35.64273215585226 37.0 34.0 40.0 29.0 41.0 23 35.49992536549241 37.0 34.0 40.0 29.0 41.0 24 35.3692709990206 37.0 34.0 40.0 27.0 41.0 25 35.17721345886483 37.0 33.0 40.0 27.0 41.0 26 35.00565264105039 37.0 33.0 40.0 27.0 41.0 27 34.81098915279722 36.0 33.0 40.0 26.0 41.0 28 34.81030219431872 36.0 33.0 40.0 26.0 41.0 29 34.63154853602407 36.0 33.0 40.0 25.0 41.0 30 34.54408684439203 36.0 33.0 40.0 25.0 41.0 31 34.38495451789158 36.0 33.0 40.0 24.0 41.0 32 33.95380557341612 36.0 33.0 40.0 23.0 41.0 33 33.56865748731775 36.0 32.0 40.0 21.0 41.0 34 33.2397019627431 36.0 31.0 40.0 18.0 41.0 35 32.90837097427084 36.0 31.0 40.0 16.0 41.0 36 32.78352012290779 36.0 31.0 40.0 15.0 41.0 37 32.75061224371874 36.0 31.0 40.0 15.0 41.0 38 32.73432426558842 36.0 31.0 40.0 15.0 41.0 39 32.60888982052728 36.0 31.0 40.0 15.0 41.0 40 32.54646359232417 36.0 30.0 40.0 15.0 41.0 41 32.552958881040944 36.0 31.0 40.0 15.0 41.0 42 32.394508055872194 36.0 30.0 40.0 13.0 41.0 43 32.426997981818715 36.0 30.0 40.0 13.0 41.0 44 32.11736846109424 35.0 30.0 40.0 12.0 41.0 45 32.05556145854767 35.0 30.0 39.0 11.0 41.0 46 31.994986166132882 35.0 30.0 39.0 11.0 41.0 47 31.934952415285018 35.0 30.0 39.0 11.0 41.0 48 31.917639777592377 35.0 30.0 39.0 11.0 41.0 49 31.913887186651433 35.0 30.0 39.0 11.0 41.0 50 31.382853195368103 35.0 29.0 39.0 10.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 1.0 11 2.0 12 5.0 13 16.0 14 67.0 15 228.0 16 717.0 17 1802.0 18 4139.0 19 7643.0 20 12487.0 21 18648.0 22 26290.0 23 36453.0 24 49612.0 25 69115.0 26 91562.0 27 103884.0 28 105663.0 29 108408.0 30 119395.0 31 136844.0 32 161459.0 33 192319.0 34 246055.0 35 276558.0 36 276676.0 37 320564.0 38 378873.0 39 369694.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.41703868892369 23.13246004004262 26.057651224760292 21.392850046273395 2 37.125707944418 23.828246255996042 26.263738768309132 12.782307031276835 3 26.409219881605594 24.51969885537951 36.36148268752281 12.709598575492082 4 23.27819796024693 26.739377262508985 36.04782515044872 13.934599626795361 5 20.5155334473342 30.361895504627178 35.79840144120037 13.324169606838254 6 19.263375065525885 39.0123077920673 32.48058459524503 9.243732547161786 7 82.5041947803354 2.936105478301261 13.395176716858506 1.1645230245048361 8 80.77209638862077 5.121724869277259 12.791776786003767 1.314401956098217 9 77.5874981261121 5.149363712734509 14.784758895229523 2.4783792659238744 10 33.37330960865516 33.931126313366704 21.301073677580852 11.394490400397281 11 26.743807181669382 25.42179732092614 32.83623006175243 14.998165435652053 12 24.7210996728601 22.607578821262713 35.649389232920974 17.02193227295621 13 22.00289485586873 25.494248969803042 36.100598970011696 16.40225720431654 14 19.192271652915192 28.18529645628938 35.64633965089027 16.97609223990516 15 18.499438716402032 24.68797158174758 40.10803866613208 16.70455103571831 16 21.167020471683667 22.974973203804208 38.76988207105783 17.088124253454293 17 20.777540695067156 22.781533400466937 35.54807890777454 20.892846996691365 18 21.66933478343634 23.12061482141808 36.509371365580364 18.70067902956522 19 23.756821834750532 24.72032925213655 33.660548135084284 17.86230077802863 20 25.09995406366436 24.28067582589904 33.192132335167685 17.427237775268917 21 23.450900605775395 24.714358491529065 34.52255262214298 17.312188280552558 22 22.626710935897464 22.977444970292254 34.11410123520913 20.281742858601152 23 20.170417064048607 26.368804894482857 33.928494042561255 19.53228399890729 24 19.797116122626583 25.03157922444956 36.85740892744274 18.313895725481117 25 20.39525151187042 25.834871232201273 35.17468166376207 18.595195592166235 26 19.635070963773853 27.103850466473702 33.99773560509004 19.263342964662407 27 19.41277248416705 26.127952115783966 35.063580575253894 19.395694824795093 28 19.086948719833615 25.988826973456757 36.52815037071682 18.396073935992803 29 20.061209926485812 24.391391704045446 36.41075751296634 19.1366408565024 30 20.717062668268714 25.21509986097116 35.43755563480902 18.630281835951106 31 22.593165533559688 25.27182208674231 33.23116698516073 18.903845394537267 32 22.974620094305916 25.13850720070519 33.370934144757555 18.515938560231334 33 22.680640386545758 25.835641652924824 31.987675836492325 19.496042124037093 34 20.716645357043458 27.20134078886588 32.32165322014997 19.760360633940692 35 20.302030604321224 27.41355959733961 32.15668688272046 20.127722915618705 36 22.917448456446028 28.034775507426374 29.56348282812459 19.484293208003002 37 21.241237668052033 29.663765925639634 29.788798788898625 19.30619761740971 38 21.47416153347109 29.568972075779865 29.18530255545344 19.771563835295606 39 21.26589113120554 29.074586677307035 29.031892528877133 20.627629662610296 40 22.387752108143957 27.328363905660698 29.751658089850956 20.532225896344386 41 20.497364358603882 27.13893671025857 29.786904837953237 22.57679409318431 42 21.35224245396977 28.178170064596568 28.818582291045047 21.651005190388616 43 21.258090621379626 27.232478626442575 29.975208503133526 21.534222249044277 44 21.034636510687502 28.10080698360705 30.33688893197538 20.527667573730067 45 20.669264482545316 28.801600934263533 29.3471230082618 21.182011574929355 46 21.329707647806018 28.686647742137616 29.427471469555062 20.556173140501308 47 20.862640084155622 28.13820448956256 29.868055820833522 21.131099605448288 48 21.472363885116145 27.169914043517856 30.67837791768761 20.67934415367839 49 21.542696877003294 26.679316546935794 30.851850983939617 20.92613559212129 50 20.518775634545793 28.2907156919614 30.473478106087576 20.71703056740523 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1800.0 1 2678.0 2 3556.0 3 37023.5 4 70491.0 5 53146.0 6 35801.0 7 36064.0 8 36327.0 9 37035.0 10 37743.0 11 37469.0 12 37195.0 13 36181.5 14 35168.0 15 33198.5 16 31229.0 17 29487.5 18 27746.0 19 26284.5 20 24823.0 21 25856.5 22 26890.0 23 27401.5 24 27913.0 25 30089.0 26 32265.0 27 36219.0 28 40173.0 29 48238.5 30 56304.0 31 63827.0 32 71350.0 33 81497.0 34 91644.0 35 102926.0 36 114208.0 37 124309.0 38 134410.0 39 146249.0 40 158088.0 41 173161.5 42 188235.0 43 201598.0 44 214961.0 45 222062.0 46 229163.0 47 223639.0 48 218115.0 49 210732.5 50 203350.0 51 193714.0 52 184078.0 53 168741.0 54 153404.0 55 142891.5 56 132379.0 57 124256.5 58 116134.0 59 108703.0 60 101272.0 61 91831.0 62 82390.0 63 72665.0 64 62940.0 65 53491.5 66 44043.0 67 36980.5 68 29918.0 69 25576.5 70 21235.0 71 18354.0 72 15473.0 73 12987.0 74 10501.0 75 8125.0 76 5749.0 77 4583.0 78 3417.0 79 2650.5 80 1884.0 81 1387.0 82 890.0 83 597.5 84 305.0 85 203.0 86 101.0 87 82.5 88 64.0 89 47.5 90 31.0 91 21.5 92 12.0 93 10.0 94 8.0 95 6.5 96 5.0 97 2.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3115181.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.14980799232105 #Duplication Level Percentage of deduplicated Percentage of total 1 79.10418626998303 22.26767654888822 2 5.6022714340054875 3.154057303762392 3 2.3011075950953446 1.9432721091481684 4 1.4215639244589875 1.6006700608932354 5 1.0348844055117707 1.4565898654701834 6 0.8486918419702215 1.4334307437666611 7 0.6903770910018731 1.360378778779992 8 0.5736313972963597 1.2918090953807488 9 0.5351106837128842 1.3556936701041598 >10 7.087577380574286 46.61047817082811 >50 0.7401161841468413 13.26965111986303 >100 0.05826718201468835 2.5229543402180847 >500 0.001748376495938017 0.3024843795018406 >1k 3.4967529918744503E-4 0.14315428438724526 >5k 0.0 0.0 >10k+ 1.1655843306248169E-4 1.2876995290079822 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39246 1.2598304881803015 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.016018330877082262 0.0 2 0.0 0.0 0.0 0.061023741477622004 0.0 3 0.0 0.0 0.0 0.11334814895185866 0.0 4 0.0 0.0 0.0 0.16711709528274601 0.0 5 0.0 0.0 0.0 0.27555381212199226 0.0 6 0.0 0.0 0.0 0.529471642257705 0.0 7 0.0 0.0 0.0 0.6807951127077367 0.0 8 0.0 0.0 0.0 1.0161849343585494 0.0 9 0.0 0.0 0.0 1.2140225559927336 0.0 10 0.0 0.0 0.0 1.4595299598963913 0.0 11 0.0 0.0 0.0 1.6110781363907907 0.0 12 0.0 0.0 0.0 1.751936725345975 0.0 13 0.0 0.0 0.0 1.8358804833491216 0.0 14 0.0 0.0 0.0 1.883293458710746 0.0 15 0.0 0.0 0.0 1.9217181922976545 0.0 16 0.0 0.0 0.0 1.9856631123520592 0.0 17 0.0 0.0 0.0 2.0554182886965475 0.0 18 0.0 0.0 0.0 2.1705319851398683 0.0 19 0.0 0.0 0.0 2.225938075508293 0.0 20 0.0 0.0 0.0 2.2885026584330093 0.0 21 0.0 0.0 0.0 2.3544378320232435 0.0 22 0.0 0.0 0.0 2.4321540225110514 0.0 23 0.0 0.0 0.0 2.5167397977838206 0.0 24 0.0 0.0 0.0 2.5887099337085067 0.0 25 0.0 0.0 0.0 2.6534573753499395 0.0 26 0.0 0.0 0.0 2.7220890214725886 0.0 27 0.0 0.0 0.0 2.7937381487624635 0.0 28 0.0 0.0 0.0 2.8704271116188753 0.0 29 0.0 0.0 0.0 2.9550128868916445 0.0 30 0.0 0.0 0.0 3.058217162983467 0.0 31 0.0 0.0 0.0 3.1509886584439233 0.0 32 0.0 0.0 0.0 3.247515954931672 0.0 33 0.0 0.0 0.0 3.3414430814774487 0.0 34 0.0 0.0 0.0 3.4362048304737347 0.0 35 0.0 0.0 0.0 3.5401153255621423 0.0 36 0.0 0.0 0.0 3.639820607534522 0.0 37 0.0 0.0 0.0 3.7465559786092686 0.0 38 0.0 0.0 0.0 3.862889507864872 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 33985 0.0 43.016033 1 GCGATAC 170 0.0 38.82353 9 CGGTCTA 275 0.0 38.4 31 GTTTTTT 37685 0.0 38.23219 2 CGTTCGA 75 0.0 38.13333 14 CGACGGT 280 0.0 37.714287 28 CGCGTAA 65 1.0913936E-11 37.230766 31 CACGACG 310 0.0 36.903225 26 AGGGCGA 3095 0.0 36.82068 6 GGGCGAT 6100 0.0 36.462296 7 CGTTAGG 430 0.0 36.32558 2 TACGGGA 1190 0.0 36.2353 4 TAGGGAC 3180 0.0 36.113205 5 CGTAAGG 465 0.0 35.95699 2 ACGGGAT 1470 0.0 35.918365 5 AGGGATT 5710 0.0 35.831875 6 TAAGGGA 3960 0.0 35.72222 4 TAGGGAT 4825 0.0 35.33679 5 AAGGGAT 5330 0.0 35.04315 5 ATTGGGA 3180 0.0 34.937107 4 >>END_MODULE