FastQCFastQC Report
Thu 26 May 2016
SRR1547214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547214_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4042026
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415201.0272076428998724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT348200.042.9259031
AGGGCGA42000.038.3428576
GGGCGAT83850.037.1520587
GTTTTTT400450.037.094272
AAGGGAT74150.036.2562335
TAAGGGA61250.036.2057154
AGGGATT89100.035.8765456
TACGGGA17450.035.8051574
GGCGATA18200.035.780228
TGGGCGA22600.035.628326
GCGCGAC9700.035.6082469
TAGGGAC46250.035.2951355
AAGGGAC49300.035.2535485
AGGGATC51350.035.2171366
ACGGGAC13750.035.25
GACCGAT28300.034.9823349
GGCGATT24600.034.967488
GTAGGGA59350.034.918284
TAGGGAT76200.034.905515
ATAGGGA68200.034.806454
Produced by FastQC (version 0.11.5)