##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547214_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4042026
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.63002464605621	31.0	30.0	31.0	28.0	34.0
2	30.965674144599763	31.0	31.0	33.0	28.0	34.0
3	31.081586560798964	31.0	31.0	34.0	28.0	34.0
4	34.92110169504105	35.0	35.0	37.0	32.0	37.0
5	34.740702805969086	35.0	35.0	37.0	32.0	37.0
6	34.77644874129954	35.0	35.0	37.0	32.0	37.0
7	35.46318331450614	35.0	35.0	37.0	35.0	37.0
8	34.43422778576882	36.0	35.0	37.0	33.0	37.0
9	36.55069487430313	39.0	37.0	39.0	32.0	39.0
10	35.94066069837255	38.0	35.0	39.0	30.0	39.0
11	35.754751948651496	37.0	35.0	39.0	30.0	39.0
12	35.57669124345068	37.0	35.0	39.0	30.0	39.0
13	35.52309435911595	37.0	35.0	39.0	30.0	39.0
14	36.467365870481785	38.0	35.0	40.0	30.0	41.0
15	36.57937925189002	38.0	35.0	40.0	30.0	41.0
16	36.59217852631329	38.0	35.0	40.0	30.0	41.0
17	36.4987560693573	38.0	35.0	40.0	30.0	41.0
18	36.35876389711496	38.0	35.0	40.0	30.0	41.0
19	36.28283390557112	38.0	35.0	40.0	30.0	41.0
20	36.090346276842354	38.0	34.0	40.0	30.0	41.0
21	35.937670118895824	38.0	34.0	40.0	30.0	41.0
22	35.89747616665504	38.0	34.0	40.0	30.0	41.0
23	35.754934530356806	37.0	34.0	40.0	29.0	41.0
24	35.64456661090255	37.0	34.0	40.0	29.0	41.0
25	35.46139584455914	37.0	34.0	40.0	27.0	41.0
26	35.3059745286151	37.0	34.0	40.0	27.0	41.0
27	35.118032145265765	37.0	33.0	40.0	27.0	41.0
28	35.115320633761385	37.0	33.0	40.0	27.0	41.0
29	34.95447827401407	37.0	33.0	40.0	26.0	41.0
30	34.912924607610144	37.0	33.0	40.0	26.0	41.0
31	34.802597014467494	37.0	33.0	40.0	25.0	41.0
32	34.44678361791834	37.0	33.0	40.0	24.0	41.0
33	34.15189412438218	37.0	33.0	40.0	23.0	41.0
34	33.91644511935351	37.0	33.0	40.0	22.0	41.0
35	33.644686105433266	37.0	32.0	40.0	20.0	41.0
36	33.560552307184565	37.0	32.0	40.0	18.0	41.0
37	33.5646378820918	37.0	32.0	40.0	18.0	41.0
38	33.545483626280486	37.0	32.0	40.0	18.0	41.0
39	33.43835368698766	37.0	32.0	40.0	18.0	41.0
40	33.3605555728736	37.0	32.0	40.0	18.0	41.0
41	33.36544099419449	37.0	32.0	40.0	18.0	41.0
42	33.20510679545357	37.0	31.0	40.0	18.0	41.0
43	33.242241390827274	37.0	32.0	40.0	18.0	41.0
44	32.86178193806769	36.0	31.0	40.0	16.0	41.0
45	32.82604763056942	36.0	31.0	40.0	16.0	41.0
46	32.78542171673314	36.0	31.0	40.0	17.0	41.0
47	32.74503800816719	36.0	31.0	40.0	17.0	41.0
48	32.75274874530743	36.0	31.0	40.0	17.0	41.0
49	32.74710652529202	36.0	31.0	40.0	17.0	41.0
50	32.20434059553303	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	5.0
13	15.0
14	64.0
15	214.0
16	679.0
17	1750.0
18	3975.0
19	7501.0
20	12536.0
21	18665.0
22	27074.0
23	38167.0
24	52911.0
25	74412.0
26	99260.0
27	114925.0
28	121515.0
29	130649.0
30	148401.0
31	175081.0
32	206724.0
33	248523.0
34	322120.0
35	370353.0
36	373470.0
37	435436.0
38	526644.0
39	530953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.558678741799284	23.28282895755742	26.63053132265849	18.527960977984804
2	35.029116586583065	24.60097980567171	27.65123232755059	12.718671280194634
3	27.15017172081525	25.047538041566288	34.86892464323584	12.93336559438262
4	23.619788690127177	27.78000438394013	34.443469685746706	14.156737240185985
5	21.485537203372765	31.60969276298569	33.53753785848978	13.36723217515177
6	19.91179670788857	39.95169749031797	30.271106618314676	9.86539918347878
7	85.35306304313728	2.6603737828504816	10.812399524396923	1.1741636496153167
8	83.42190277845813	5.098903371724972	10.210894239670898	1.2682996101460011
9	80.09686231607614	5.392469024197272	12.012441285632502	2.4982273740940806
10	31.295197012587252	38.561849923775846	18.820586507855218	11.322366555781679
11	26.132513744345037	25.65639112662808	32.737790405108726	15.473304723918154
12	25.305799616331022	23.54885891382193	33.857352723609395	17.287988746237655
13	22.00403955837988	26.52204612241485	34.72038527213827	16.75352904706699
14	19.33473461081151	29.235017290833852	33.91591246567934	17.514335632675294
15	18.60336870668323	25.65666326738126	38.68525338530727	17.05471464062824
16	21.09778611023284	24.27925995527985	37.40819579092267	17.214758143564637
17	20.68000057396959	23.502644465918824	34.36036779575391	21.456987164357677
18	21.64055847240963	23.780302254364518	35.71646990890212	18.862669364323732
19	23.692301830814547	25.464531895638476	32.52821728509416	18.31494898845282
20	25.387516062489457	25.28217285094158	31.781685718993398	17.548625367575568
21	23.5649646983963	25.72600967930439	33.26321997928762	17.445805643011695
22	22.906359335640097	23.510932388856478	32.938061259378344	20.64464701612508
23	20.130127812141733	27.372881817187718	33.028115108611374	19.468875262059175
24	19.773821346027955	25.633457083155825	36.09952038903263	18.49320118178359
25	20.387820365331642	26.53048248576333	34.14253149287016	18.93916565603487
26	20.00692227115808	28.39843187550006	32.21716040421313	19.377485449128727
27	19.705514016980594	26.969965062075307	33.746368776450225	19.578152144493874
28	18.747430125387616	26.865883594019436	35.90808173920702	18.47860454138593
29	19.766473545692183	25.429326778204793	35.33035166028126	19.47384801582177
30	20.407142358807192	26.14411683645776	34.40868514947702	19.04005565525803
31	22.5020818767618	26.47684601731904	31.911843219217296	19.109228886701867
32	22.565985473621396	26.792158189977005	32.08898210946688	18.55287422693471
33	22.41685234088054	26.868382340934964	31.06892434635502	19.64584097182947
34	20.26275931921269	27.966198139249972	31.854075159338414	19.916967382198926
35	20.532104444652262	27.2831495888448	31.88586614732315	20.29887981917979
36	22.75737464331006	28.394102363517703	29.393427949251194	19.455095043921045
37	20.992393418548026	29.817670643385274	29.444021389273594	19.745914548793106
38	21.031581687005477	30.047654319888096	29.015102822198568	19.905661170907855
39	21.377447844224655	29.095161683769476	28.934153318162725	20.59323715384315
40	22.422765217245015	27.679708146360266	29.660496988391465	20.237029648003254
41	20.142992647746453	27.658926488844948	29.940480343273396	22.2576005201352
42	21.2047374262313	28.772650151186557	28.409490686106427	21.61312173647572
43	21.171412554001385	27.78997463153379	29.481428372801165	21.55718444166366
44	21.26787408096831	28.44284029840481	29.988599776448744	20.300685844178144
45	20.718273459893627	29.16277629089966	29.071906019407102	21.04704422979961
46	21.419011159255284	29.129748299491393	29.10458764985678	20.34665289139654
47	21.132669606776403	28.507956158619464	29.52482245289862	20.83455178170551
48	21.408966691456214	27.77901478120131	30.28686604193046	20.52515248541202
49	21.108919141044616	27.518749260890456	30.92402176532264	20.448309832742293
50	20.443980320759934	28.788508535076225	30.253714350179834	20.513796793984007
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2022.0
1	3208.0
2	4394.0
3	39990.0
4	75586.0
5	56598.0
6	37610.0
7	37502.5
8	37395.0
9	38020.0
10	38645.0
11	38197.0
12	37749.0
13	37193.5
14	36638.0
15	34711.0
16	32784.0
17	31800.5
18	30817.0
19	29675.5
20	28534.0
21	29763.5
22	30993.0
23	32575.0
24	34157.0
25	38626.0
26	43095.0
27	51853.0
28	60611.0
29	70124.0
30	79637.0
31	91955.0
32	104273.0
33	119607.0
34	134941.0
35	147753.0
36	160565.0
37	174105.5
38	187646.0
39	204526.5
40	221407.0
41	237371.5
42	253336.0
43	275251.0
44	297166.0
45	301641.0
46	306116.0
47	297057.5
48	287999.0
49	284290.0
50	280581.0
51	263032.5
52	245484.0
53	226354.0
54	207224.0
55	190522.0
56	173820.0
57	160041.5
58	146263.0
59	134634.5
60	123006.0
61	109394.5
62	95783.0
63	84118.0
64	72453.0
65	58272.0
66	44091.0
67	36956.0
68	29821.0
69	25424.0
70	21027.0
71	18171.5
72	15316.0
73	13066.5
74	10817.0
75	8377.5
76	5938.0
77	4554.5
78	3171.0
79	2540.5
80	1910.0
81	1287.0
82	664.0
83	513.0
84	362.0
85	234.0
86	106.0
87	78.0
88	50.0
89	32.5
90	15.0
91	11.0
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4042026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.021563083866397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.35346243931174	19.30215766993016
2	5.879434240306493	2.8246640100193305
3	2.1219196589595524	1.5291548083977946
4	1.2139197484746445	1.1664099926693958
5	0.846387985040811	1.0165781188042207
6	0.6515253029019977	0.9390393698637299
7	0.5551492438927472	0.9334886808191251
8	0.43468417980299995	0.8353434757357189
9	0.38787941814740917	0.8385722920765242
>10	5.599294289206442	32.881595799669675
>50	1.6964225991830033	28.014285611718424
>100	0.25675021490565314	8.074796966572585
>500	0.002747922550997984	0.4535607077060226
>1k	3.170679866506241E-4	0.13623323579808158
>5k	0.0	0.0
>10k+	1.0568932888354136E-4	1.0541192602192224
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41520	1.0272076428998724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015314102383309757	0.0
2	0.0	0.0	0.0	0.05724851844099964	0.0
3	0.0	0.0	0.0	0.10264654408457541	0.0
4	0.0	0.0	0.0	0.15405640636651027	0.0
5	0.0	0.0	0.0	0.24680692306284027	0.0
6	0.0	0.0	0.0	0.4737475711437779	0.0
7	0.0	0.0	0.0	0.6037566309568518	0.0
8	0.0	0.0	0.0	0.9158031145766999	0.0
9	0.0	0.0	0.0	1.0891567743502888	0.0
10	0.0	0.0	0.0	1.3233215224246455	0.0
11	0.0	0.0	0.0	1.4599856606563144	0.0
12	0.0	0.0	0.0	1.5918502256046845	0.0
13	0.0	0.0	0.0	1.6738388125162975	0.0
14	0.0	0.0	0.0	1.7164164703542233	0.0
15	0.0	0.0	0.0	1.7533781326493199	0.0
16	0.0	0.0	0.0	1.8148077226618533	0.0
17	0.0	0.0	0.0	1.8868755421167505	0.0
18	0.0	0.0	0.0	1.9989480522886296	0.0
19	0.0	0.0	0.0	2.05300510189692	0.0
20	0.0	0.0	0.0	2.114014110745453	0.0
21	0.0	0.0	0.0	2.1847954466398782	0.0
22	0.0	0.0	0.0	2.261860759925839	0.0
23	0.0	0.0	0.0	2.353473233472521	0.0
24	2.47400684706135E-5	0.0	0.0	2.422027963204591	0.0
25	2.47400684706135E-5	0.0	0.0	2.488479787116659	0.0
26	2.47400684706135E-5	0.0	0.0	2.552853445277195	0.0
27	2.47400684706135E-5	0.0	0.0	2.621729795899383	0.0
28	2.47400684706135E-5	0.0	0.0	2.699364130760168	0.0
29	2.47400684706135E-5	0.0	0.0	2.7800909741797803	0.0
30	2.47400684706135E-5	0.0	0.0	2.890951220996599	0.0
31	2.47400684706135E-5	0.0	0.0	2.9838749181722237	0.0
32	2.47400684706135E-5	0.0	0.0	3.0859771807504455	0.0
33	4.9480136941227E-5	0.0	0.0	3.1898607282585516	0.0
34	4.9480136941227E-5	0.0	0.0	3.28981060487983	0.0
35	4.9480136941227E-5	0.0	0.0	3.398691646219	0.0
36	4.9480136941227E-5	0.0	0.0	3.5014866307143993	0.0
37	4.9480136941227E-5	0.0	0.0	3.6086853473975675	0.0
38	4.9480136941227E-5	0.0	0.0	3.726794434276276	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34820	0.0	42.925903	1
AGGGCGA	4200	0.0	38.342857	6
GGGCGAT	8385	0.0	37.152058	7
GTTTTTT	40045	0.0	37.09427	2
AAGGGAT	7415	0.0	36.256233	5
TAAGGGA	6125	0.0	36.205715	4
AGGGATT	8910	0.0	35.876545	6
TACGGGA	1745	0.0	35.805157	4
GGCGATA	1820	0.0	35.78022	8
TGGGCGA	2260	0.0	35.62832	6
GCGCGAC	970	0.0	35.608246	9
TAGGGAC	4625	0.0	35.295135	5
AAGGGAC	4930	0.0	35.253548	5
AGGGATC	5135	0.0	35.217136	6
ACGGGAC	1375	0.0	35.2	5
GACCGAT	2830	0.0	34.982334	9
GGCGATT	2460	0.0	34.96748	8
GTAGGGA	5935	0.0	34.91828	4
TAGGGAT	7620	0.0	34.90551	5
ATAGGGA	6820	0.0	34.80645	4
>>END_MODULE