FastQCFastQC Report
Thu 26 May 2016
SRR1547213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547213_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4135709
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399390.9657110788017241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT274350.042.468381
CGTTAGG6300.038.41272
TCACGAC3850.037.14285725
TCGATAG1600.037.1250041
ACGGGAT20800.037.0192345
AATACCG601.9826984E-1036.6666641
TACGGGA17150.036.4314884
GTTTTTT323000.036.1944272
TAACGCC1950.036.10256612
CGGTCTA3900.036.10256631
TAAGGGA59650.035.7384764
GGGCGAT85600.035.6728977
AACGGGA17000.035.4588244
CGCGATA250.002353449435.220
GTCGTAC250.002353449435.223
CCGGTAT505.9519152E-835.242
ACGACGT250.002353449435.214
TAGGGAT65350.034.9778145
AAGGGAT73400.034.948235
AGGGCGA45050.034.916766
Produced by FastQC (version 0.11.5)