##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547213_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4135709
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.730110121384264	31.0	31.0	31.0	28.0	34.0
2	31.07067518531889	31.0	31.0	33.0	28.0	34.0
3	31.200095316183997	31.0	31.0	34.0	28.0	34.0
4	35.03325475752767	35.0	35.0	37.0	32.0	37.0
5	34.86427961928656	35.0	35.0	37.0	32.0	37.0
6	34.89668204411868	35.0	35.0	37.0	32.0	37.0
7	35.515728742036735	36.0	35.0	37.0	35.0	37.0
8	34.49396608900675	37.0	35.0	37.0	33.0	37.0
9	36.628866537756885	39.0	37.0	39.0	32.0	39.0
10	36.10550234554704	38.0	35.0	39.0	31.0	39.0
11	35.853825063610614	37.0	35.0	39.0	30.0	39.0
12	35.62500238774053	37.0	35.0	39.0	30.0	39.0
13	35.51744162850916	37.0	35.0	39.0	30.0	39.0
14	36.46259154113599	38.0	35.0	40.0	30.0	41.0
15	36.623873681634755	38.0	35.0	40.0	31.0	41.0
16	36.66988151245651	38.0	35.0	40.0	31.0	41.0
17	36.576102912463135	38.0	35.0	40.0	31.0	41.0
18	36.48071080436269	38.0	35.0	40.0	30.0	41.0
19	36.41123081918965	38.0	35.0	40.0	30.0	41.0
20	36.24136732057309	38.0	34.0	40.0	30.0	41.0
21	36.08990477811664	38.0	34.0	40.0	30.0	41.0
22	36.04727726249598	38.0	34.0	40.0	30.0	41.0
23	35.923714168477524	38.0	34.0	40.0	30.0	41.0
24	35.79274291300476	38.0	34.0	40.0	29.0	41.0
25	35.628359006883706	37.0	34.0	40.0	29.0	41.0
26	35.4516251022497	37.0	34.0	40.0	28.0	41.0
27	35.265828180851216	37.0	34.0	40.0	27.0	41.0
28	35.26713629996695	37.0	34.0	40.0	27.0	41.0
29	35.11520225431722	37.0	33.0	40.0	27.0	41.0
30	35.11953597315478	37.0	33.0	40.0	27.0	41.0
31	35.03683044430834	37.0	33.0	40.0	27.0	41.0
32	34.75792373206142	37.0	33.0	40.0	25.0	41.0
33	34.51957113036725	37.0	33.0	40.0	24.0	41.0
34	34.3267717820572	37.0	33.0	40.0	23.0	41.0
35	34.074149075769114	37.0	33.0	40.0	23.0	41.0
36	33.99617211946005	37.0	33.0	40.0	22.0	41.0
37	33.96817571062181	37.0	33.0	40.0	22.0	41.0
38	33.96438168159317	37.0	33.0	40.0	22.0	41.0
39	33.864475958052175	37.0	33.0	40.0	22.0	41.0
40	33.788339798569	37.0	33.0	40.0	21.0	41.0
41	33.79107258271798	37.0	33.0	40.0	21.0	41.0
42	33.645042966030736	36.0	32.0	40.0	21.0	41.0
43	33.67859344068937	36.0	32.0	40.0	21.0	41.0
44	33.390219911507316	36.0	32.0	40.0	20.0	41.0
45	33.3225120529515	36.0	32.0	40.0	20.0	41.0
46	33.25994454638854	36.0	32.0	40.0	20.0	41.0
47	33.205508898232445	36.0	31.0	40.0	20.0	41.0
48	33.18533073772841	36.0	31.0	40.0	20.0	41.0
49	33.18243522452861	36.0	31.0	39.0	20.0	41.0
50	32.63415873795763	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	6.0
12	6.0
13	11.0
14	34.0
15	170.0
16	494.0
17	1311.0
18	2968.0
19	5735.0
20	10015.0
21	15660.0
22	23254.0
23	33225.0
24	46352.0
25	65440.0
26	87088.0
27	102307.0
28	111628.0
29	124073.0
30	145735.0
31	174329.0
32	212223.0
33	262166.0
34	351841.0
35	405703.0
36	394393.0
37	462696.0
38	550687.0
39	546155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.805721582442093	23.848147923366948	27.889220445635804	17.45691004855516
2	33.54723458541208	25.084671092671172	27.945994266037577	13.422100055879174
3	27.85004941111669	25.315828555635804	33.52583559433219	13.30828643891531
4	24.483879305821567	28.190909950385773	32.74164115511996	14.583569588672704
5	21.93892268532433	32.28820016108484	31.85656921219554	13.916307941395297
6	20.05709299179415	40.86491578590273	29.089715935042822	9.988275287260299
7	86.56017625998348	3.088249197416936	8.995603897663013	1.3559706449365756
8	84.89366635805372	5.268044729452677	8.429147215144974	1.4091416973486286
9	81.84572463874997	5.272203629413965	10.332593516613475	2.5494782152225897
10	37.626124081747534	35.20216243454266	16.105388459391122	11.066325024318683
11	28.449414598560974	25.39100309040119	30.190470364331727	15.969111946706116
12	26.858635363368165	23.005583806791048	32.22177865995891	17.914002169881876
13	22.54138286808864	27.77765553620915	32.61288451387658	17.06807708182563
14	19.63223234516742	29.908463095445057	32.543126220921245	17.91617833846627
15	18.821778805036814	24.58441829442062	38.499323816061526	18.09447908448104
16	21.546801286067275	23.11654422494426	36.719774045997916	18.61688044299055
17	21.317844171337974	23.201342260782855	32.15151259433389	23.329300973545287
18	22.58916185834158	23.627605327163977	34.04216786045634	19.74106495403811
19	25.898437244980244	24.635751693361403	30.252249372477607	19.213561689180743
20	26.717933974561557	25.463203528101232	29.172893934268586	18.645968563068628
21	24.45556493457349	25.846499354766017	31.317459714888063	18.38047599577243
22	24.22450419021261	23.535093982676248	30.34442220185221	21.895979625258935
23	21.387892620104555	27.33850471587822	30.280974797791625	20.992627866225597
24	20.54912470872588	25.645324658964157	34.781920101245035	19.023630531064928
25	21.54140922390816	26.215746804235984	32.66204174423297	19.580802227622883
26	20.4972835371154	28.427000062141705	30.22420097738985	20.851515423353046
27	20.325414578250065	27.69341363234212	31.5904721536259	20.390699635781917
28	19.653848953105744	26.614034014482158	34.41584018604791	19.316276846364193
29	20.484250705259967	25.21785261003615	33.884178021229246	20.41371866347463
30	20.988323888358686	25.96848569374683	32.950650057825634	20.092540360068853
31	23.969118717008378	25.680312613871042	30.39263642582203	19.957932243298547
32	25.1623119518322	25.663967169837143	29.92091561567799	19.252805262652668
33	24.319288421888487	25.843162562936612	29.650055165873614	20.187493849301294
34	21.58070115668196	27.163347324485354	30.47726520410406	20.778686314728624
35	21.6837306493276	26.796469480807282	30.85882009590133	20.660979773963785
36	24.9900802982028	26.964929108890402	28.40816411406122	19.636826478845588
37	21.949368294529425	29.323799135770916	28.625297379481967	20.101535190217685
38	22.19031851612384	29.408016859987008	27.7639698537784	20.63769477011076
39	21.834152257811176	28.8063304260527	27.976194650058794	21.38332266607733
40	23.071739331756657	26.528172074002303	28.734009090097974	21.666079504143063
41	20.56921799865513	26.65364028271815	28.81496256143747	23.96217915718925
42	22.126097363233242	27.360435659278735	27.931341397569316	22.582125579918703
43	21.593395473424266	26.693198191652268	29.069743543368258	22.643662791555208
44	21.663105407077722	27.43285371383722	29.936801646344076	20.96723923274099
45	20.68634422779746	28.93453093532451	28.720516844874723	21.658607992003308
46	22.12435642836573	28.160854644270184	29.02119080428531	20.693598123078775
47	21.71354415893381	27.290072875049958	29.202852521780425	21.793530444235802
48	21.82264274396482	26.70876988685616	30.27282625542561	21.19576111375341
49	21.71349579963194	26.57210649975615	30.292266694779542	21.422131005832373
50	20.621155888869357	28.41887569942663	29.65839714544713	21.301571266256886
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2563.0
1	3841.5
2	5120.0
3	35921.0
4	66722.0
5	47889.5
6	29057.0
7	29057.5
8	29058.0
9	29601.5
10	30145.0
11	30408.0
12	30671.0
13	29604.5
14	28538.0
15	27708.0
16	26878.0
17	26331.5
18	25785.0
19	24520.0
20	23255.0
21	24769.5
22	26284.0
23	29383.0
24	32482.0
25	35620.5
26	38759.0
27	47184.0
28	55609.0
29	66772.0
30	77935.0
31	88369.0
32	98803.0
33	110931.0
34	123059.0
35	137117.0
36	151175.0
37	161432.0
38	171689.0
39	191840.0
40	211991.0
41	239035.5
42	266080.0
43	282284.0
44	298488.0
45	307223.0
46	315958.0
47	307501.0
48	299044.0
49	288173.0
50	277302.0
51	259912.0
52	242522.0
53	225984.5
54	209447.0
55	199835.0
56	190223.0
57	177344.0
58	164465.0
59	155040.0
60	145615.0
61	134133.0
62	122651.0
63	108661.0
64	94671.0
65	82264.0
66	69857.0
67	60273.0
68	50689.0
69	44566.5
70	38444.0
71	32146.0
72	25848.0
73	21064.5
74	16281.0
75	13735.5
76	11190.0
77	8543.0
78	5896.0
79	4309.0
80	2722.0
81	2181.5
82	1641.0
83	1150.5
84	660.0
85	452.0
86	244.0
87	174.5
88	105.0
89	77.0
90	49.0
91	37.5
92	26.0
93	18.5
94	11.0
95	6.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4135709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.547452840356147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1739194388931	17.49091200976418
2	5.977245039502582	2.575888112078692
3	2.097390247234395	1.3558025232031814
4	1.103178716888485	0.9508276550655695
5	0.7905544154617229	0.8517216992448398
6	0.5733803915446604	0.7412932167836107
7	0.4560613034060583	0.6878871599217379
8	0.38514675858489583	0.6639145293779262
9	0.32848237533985947	0.6370162642371409
>10	4.546540567062078	24.065978653943056
>50	1.8935791669149868	29.234947642719177
>100	0.6707331291900129	19.07798480066929
>500	0.0028700378615501318	0.3979656736119985
>1k	8.036106012334933E-4	0.2798969167651235
>5k	0.0	0.0
>10k+	1.1480151446192764E-4	0.9879631426144293
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39939	0.9657110788017241	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017941300995790565	0.0
2	0.0	0.0	0.0	0.06910544238001272	0.0
3	0.0	0.0	0.0	0.12094661399049111	0.0
4	0.0	0.0	0.0	0.17878433903352484	0.0
5	0.0	0.0	0.0	0.27368946896408813	0.0
6	0.0	0.0	0.0	0.5248193236032807	0.0
7	0.0	0.0	0.0	0.6586295118926404	0.0
8	0.0	0.0	0.0	1.0228959532694395	0.0
9	0.0	0.0	0.0	1.224578421740988	0.0
10	0.0	0.0	0.0	1.48484818443464	0.0
11	0.0	0.0	0.0	1.648762038141465	0.0
12	0.0	0.0	0.0	1.798458257096909	0.0
13	0.0	0.0	0.0	1.8847554312936428	0.0
14	0.0	0.0	0.0	1.9306000494715658	0.0
15	0.0	0.0	0.0	1.9689247962078569	0.0
16	0.0	0.0	0.0	2.0337504403718927	0.0
17	0.0	0.0	0.0	2.108658998976959	0.0
18	0.0	0.0	0.0	2.219643596781108	0.0
19	0.0	0.0	0.0	2.276078902069754	0.0
20	0.0	0.0	0.0	2.341315600299731	0.0
21	0.0	0.0	0.0	2.4125246723113256	0.0
22	0.0	0.0	0.0	2.491326154717365	0.0
23	0.0	0.0	0.0	2.5816129713188234	0.0
24	0.0	0.0	0.0	2.653789229367927	0.0
25	0.0	0.0	0.0	2.71832471772071	0.0
26	0.0	0.0	0.0	2.7849396560541373	0.0
27	0.0	0.0	0.0	2.8603076280270203	0.0
28	0.0	0.0	0.0	2.9390607511311844	0.0
29	0.0	0.0	0.0	3.0190712160841104	0.0
30	0.0	0.0	0.0	3.1336102225761047	0.0
31	0.0	0.0	0.0	3.222301182215673	0.0
32	0.0	0.0	0.0	3.3115240941758715	0.0
33	0.0	0.0	0.0	3.4003601317210665	0.0
34	0.0	0.0	0.0	3.4934276081803626	0.0
35	0.0	0.0	0.0	3.5952239386281772	0.0
36	0.0	0.0	0.0	3.6931031656240805	2.4179650937723133E-5
37	0.0	0.0	0.0	3.7944884420059535	2.4179650937723133E-5
38	0.0	0.0	0.0	3.906488585149487	2.4179650937723133E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	27435	0.0	42.46838	1
CGTTAGG	630	0.0	38.4127	2
TCACGAC	385	0.0	37.142857	25
TCGATAG	160	0.0	37.125004	1
ACGGGAT	2080	0.0	37.019234	5
AATACCG	60	1.9826984E-10	36.666664	1
TACGGGA	1715	0.0	36.431488	4
GTTTTTT	32300	0.0	36.194427	2
TAACGCC	195	0.0	36.102566	12
CGGTCTA	390	0.0	36.102566	31
TAAGGGA	5965	0.0	35.738476	4
GGGCGAT	8560	0.0	35.672897	7
AACGGGA	1700	0.0	35.458824	4
CGCGATA	25	0.0023534494	35.2	20
GTCGTAC	25	0.0023534494	35.2	23
CCGGTAT	50	5.9519152E-8	35.2	42
ACGACGT	25	0.0023534494	35.2	14
TAGGGAT	6535	0.0	34.977814	5
AAGGGAT	7340	0.0	34.94823	5
AGGGCGA	4505	0.0	34.91676	6
>>END_MODULE