FastQCFastQC Report
Thu 26 May 2016
SRR1547212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547212_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences6477427
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537000.8290328860518228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT545850.042.6901171
CGACGGT4200.038.23809428
CACGACG4550.037.2307726
ACGGGAT28600.037.1538475
AGGGCGA60500.037.1272746
GTTTTTT620300.037.0910842
GGGCGAT117350.036.819777
TAGGGAT130050.036.6243745
TAGGGCG26600.036.4736865
TACGGGA23000.036.252174
GGGACCG48950.036.134837
TAGGGAC73200.036.035525
ACGGGAC21800.036.0275235
AGGGATT148650.035.948876
GACCGAT41700.035.7697839
AACGGGA24100.035.6929474
CGTTAGG9600.035.5208362
GTAGGGA99800.035.5130274
TGGGCGA33700.035.382796
TTAGGGA98600.035.231244
Produced by FastQC (version 0.11.5)