##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547212_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	6477427
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.59821639055137	31.0	30.0	31.0	27.0	34.0
2	30.936393416706974	31.0	31.0	33.0	28.0	34.0
3	31.053855180459774	31.0	31.0	34.0	28.0	34.0
4	34.8878418853659	35.0	35.0	37.0	32.0	37.0
5	34.70430110597927	35.0	35.0	37.0	32.0	37.0
6	34.74271790326622	35.0	35.0	37.0	32.0	37.0
7	35.450536455293125	35.0	35.0	37.0	35.0	37.0
8	34.43026606089116	36.0	35.0	37.0	33.0	37.0
9	36.545265581534146	39.0	37.0	39.0	32.0	39.0
10	35.92135164163178	38.0	35.0	39.0	30.0	39.0
11	35.67525685739106	37.0	35.0	39.0	30.0	39.0
12	35.48958374984388	37.0	35.0	39.0	30.0	39.0
13	35.39289659304536	37.0	35.0	39.0	30.0	39.0
14	36.32335092313661	38.0	35.0	40.0	30.0	41.0
15	36.478086900863566	38.0	35.0	40.0	30.0	41.0
16	36.519832334660045	38.0	35.0	40.0	30.0	41.0
17	36.43597017766468	38.0	35.0	40.0	30.0	41.0
18	36.29714005267832	38.0	35.0	40.0	30.0	41.0
19	36.21415494146055	38.0	34.0	40.0	30.0	41.0
20	36.02385746686146	38.0	34.0	40.0	30.0	41.0
21	35.868600448912815	38.0	34.0	40.0	29.0	41.0
22	35.83614311670359	38.0	34.0	40.0	29.0	41.0
23	35.70196175117064	37.0	34.0	40.0	29.0	41.0
24	35.601284584141204	37.0	34.0	40.0	29.0	41.0
25	35.416621291139215	37.0	34.0	40.0	27.0	41.0
26	35.258770496371476	37.0	34.0	40.0	27.0	41.0
27	35.04141227681917	37.0	33.0	40.0	27.0	41.0
28	35.05631047636662	37.0	33.0	40.0	27.0	41.0
29	34.90494420083777	37.0	33.0	40.0	26.0	41.0
30	34.867996351020246	37.0	33.0	40.0	26.0	41.0
31	34.753420918522124	37.0	33.0	40.0	25.0	41.0
32	34.39093748181184	37.0	33.0	40.0	24.0	41.0
33	34.09620486653111	37.0	33.0	40.0	23.0	41.0
34	33.881850463154585	37.0	33.0	40.0	21.0	41.0
35	33.60877089004631	37.0	32.0	40.0	20.0	41.0
36	33.527210418581326	37.0	32.0	40.0	18.0	41.0
37	33.5048039908439	37.0	32.0	40.0	18.0	41.0
38	33.512170804858165	37.0	32.0	40.0	18.0	41.0
39	33.410669082029024	37.0	32.0	40.0	18.0	41.0
40	33.37082393981437	37.0	32.0	40.0	18.0	41.0
41	33.39111502144293	37.0	32.0	40.0	18.0	41.0
42	33.24131171219683	37.0	31.0	40.0	18.0	41.0
43	33.286285897162564	37.0	32.0	40.0	18.0	41.0
44	32.99505513531839	36.0	31.0	40.0	17.0	41.0
45	32.939669408856325	36.0	31.0	40.0	17.0	41.0
46	32.8838274827335	36.0	31.0	40.0	17.0	41.0
47	32.84129670623845	36.0	31.0	40.0	17.0	41.0
48	32.834320170648006	36.0	31.0	40.0	17.0	41.0
49	32.832372174939216	36.0	31.0	40.0	17.0	41.0
50	32.28396769272737	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	7.0
12	12.0
13	28.0
14	101.0
15	379.0
16	1114.0
17	2944.0
18	6785.0
19	12747.0
20	21057.0
21	31611.0
22	45401.0
23	63169.0
24	87310.0
25	121744.0
26	160205.0
27	182710.0
28	192603.0
29	207026.0
30	236977.0
31	279138.0
32	333258.0
33	402474.0
34	522667.0
35	599373.0
36	580814.0
37	686507.0
38	836623.0
39	862641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.984695003123925	23.513117168283024	27.08367072295836	18.41851710563469
2	33.90460440542209	24.802008575318563	28.141667980202634	13.151719039056712
3	26.594927275907548	25.100954437618515	35.09679074731371	13.207327539160223
4	23.345427127160214	28.14135921562682	34.11081282737729	14.402400829835674
5	21.07072762070495	32.02537056766522	33.68184620220344	13.222055609426398
6	19.637519650935474	40.49776246031024	30.396483047975686	9.4682348407786
7	85.57913504853084	2.84957283192848	10.402834335300112	1.1684577842405635
8	83.82854797128552	5.036629513539867	9.857833982536585	1.2769885326380368
9	80.56567831640558	5.332904562259057	11.788477122165947	2.3129399991694233
10	33.69597218154678	37.09665890483984	18.329469401970876	10.877899511642507
11	25.70015841166562	25.082459439527454	33.3505726888161	15.866809459990828
12	25.427534729453534	23.653852061937556	33.70224010243574	17.216373106173176
13	20.977727730470757	28.447221404424937	34.45758632247033	16.11746454263398
14	18.501451270697455	31.27244814955074	33.60005446607117	16.62604611368063
15	17.554254181482865	26.360127254232275	39.8058056076896	16.279812956595265
16	20.05220591447808	24.35085721537271	38.75538852078147	16.841548349367734
17	19.96567464210712	23.73678622700032	34.18610506918874	22.11143406170382
18	21.24613677622303	24.20021097883465	35.84946615376754	18.704186091174783
19	23.268344050809063	25.66736761371452	32.77878700910099	18.285501326375428
20	24.67299129731605	25.863371366439175	32.20527224776134	17.258365088483437
21	22.464490915914606	26.126068267538948	33.55778459564268	17.851656220903763
22	21.90981079369941	24.584036223024977	32.91436553433948	20.591787448936127
23	19.04685301740954	27.630616292549497	33.030955038165615	20.291575651875352
24	19.008303760119567	26.177045299005297	36.98312617031423	17.831524770560904
25	19.006790813698093	27.268682456784155	34.96741839004901	18.757108339468743
26	19.120971953832903	29.568005938160326	32.720476818959135	18.59054528904764
27	18.819880177731065	28.80981908402827	33.73614245285975	18.63415828538091
28	18.37419395077706	27.47200392995552	36.25334565715677	17.90045646211065
29	18.841169495233213	25.294765961854914	36.28777290735967	19.576291635552202
30	19.595620297997954	27.194440014530464	34.28704020902127	18.92289947845032
31	21.98220064849824	27.20816459992525	31.857757717686358	18.951877033890156
32	22.862148813101253	26.65941275756562	32.12536397554152	18.353074453791606
33	22.33124356322348	27.213830429891374	31.042943440350623	19.411982566534522
34	20.063460383266378	27.705815904988203	31.8325625283002	20.398161183445218
35	19.8661906957809	27.400092660249204	31.67941529869808	21.05430134527182
36	22.878991920711726	27.808495564674057	29.93088150588189	19.381631008732324
37	20.554843767440374	29.59499196208618	30.485376369351595	19.36478790112185
38	20.40507750994338	29.879765530356423	29.616404785418656	20.098752174281547
39	20.157540949515912	29.609920729326628	29.986613511815722	20.245924809341734
40	21.36389958543724	27.871607043969775	30.031183678334006	20.733309692258977
41	19.184901659254518	27.6793856572988	30.464611951628324	22.67110073181836
42	20.697801766040744	28.27154979901742	29.20303077132324	21.827617663618597
43	20.796992386019944	27.961349467929164	30.16893899383196	21.07271915221893
44	20.329275806581844	29.20982359199108	30.097737882650012	20.36316271877707
45	19.536630825789313	30.40989886879466	28.968014614444904	21.085455690971123
46	20.6528456438027	30.192482292737534	29.156515387977354	19.99815667548241
47	20.54144338485019	28.37802726298575	30.024159284234308	21.05637006792975
48	21.07534365111332	27.05839834242825	30.930908214017695	20.935349792440732
49	20.2763226818303	27.540472474641554	31.136005083499974	21.04719976002817
50	19.74941593320928	29.599299227918742	29.896330749848666	20.75495408902331
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4016.0
1	5235.0
2	6454.0
3	53709.0
4	100964.0
5	78401.5
6	55839.0
7	56666.0
8	57493.0
9	59228.0
10	60963.0
11	60512.0
12	60061.0
13	59156.0
14	58251.0
15	55986.5
16	53722.0
17	51995.5
18	50269.0
19	49204.5
20	48140.0
21	47992.5
22	47845.0
23	52246.5
24	56648.0
25	64665.0
26	72682.0
27	86029.5
28	99377.0
29	113767.5
30	128158.0
31	149378.0
32	170598.0
33	195951.5
34	221305.0
35	248722.0
36	276139.0
37	293382.0
38	310625.0
39	344435.5
40	378246.0
41	419156.0
42	460066.0
43	488063.5
44	516061.0
45	521603.0
46	527145.0
47	517090.0
48	507035.0
49	487187.0
50	467339.0
51	431041.5
52	394744.0
53	360226.5
54	325709.0
55	291498.5
56	257288.0
57	231508.5
58	205729.0
59	183298.0
60	160867.0
61	139563.0
62	118259.0
63	100094.5
64	81930.0
65	65336.5
66	48743.0
67	40653.0
68	32563.0
69	27222.0
70	21881.0
71	18091.5
72	14302.0
73	11435.0
74	8568.0
75	6581.5
76	4595.0
77	4055.0
78	3515.0
79	2623.0
80	1731.0
81	1287.0
82	843.0
83	598.0
84	353.0
85	281.5
86	210.0
87	149.5
88	89.0
89	67.0
90	45.0
91	31.0
92	17.0
93	10.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	6477427.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.939717108554106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.56944166963216	18.11550192042989
2	6.431078823220428	2.8219210016853844
3	2.103696531285698	1.3846352033596425
4	1.1211722953044103	0.9839281195570818
5	0.6914410444488783	0.7585010456225787
6	0.5007456987174205	0.6591731383911282
7	0.4106371643327327	0.63064842538033
8	0.3041600591746901	0.5338548523211022
9	0.26814915454235966	0.5294804934201595
>10	3.301416797249558	17.260885708533795
>50	1.232046026055617	19.719148721666755
>100	1.0617575342361736	34.93313620593735
>500	0.0032470183594340263	0.4753993478909731
>1k	8.658715203698242E-4	0.2636938605981408
>5k	7.215596003081766E-5	0.0799772698410154
>10k+	7.215596003081766E-5	0.8501146853647125
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53700	0.8290328860518228	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01900445964115072	0.0
2	0.0	0.0	0.0	0.0707688407758204	0.0
3	0.0	0.0	0.0	0.12168411932701055	0.0
4	0.0	0.0	0.0	0.19848930755993083	0.0
5	0.0	0.0	0.0	0.31869135692304984	0.0
6	0.0	0.0	0.0	0.5992657269622645	0.0
7	0.0	0.0	0.0	0.7577391454971241	0.0
8	0.0	0.0	0.0	1.1603372758967412	0.0
9	0.0	0.0	0.0	1.3835586259791117	0.0
10	0.0	0.0	0.0	1.6636698491546102	0.0
11	0.0	0.0	0.0	1.835003312271987	0.0
12	0.0	0.0	0.0	1.9967187588528594	0.0
13	0.0	0.0	0.0	2.0874955441412153	0.0
14	0.0	0.0	0.0	2.1323899134640962	0.0
15	0.0	0.0	0.0	2.1733012197590185	0.0
16	0.0	0.0	0.0	2.2458763333033316	0.0
17	0.0	0.0	0.0	2.329412589288926	0.0
18	1.5438228790536735E-5	0.0	0.0	2.4608845456691366	0.0
19	1.5438228790536735E-5	0.0	0.0	2.5238570809057363	0.0
20	1.5438228790536735E-5	0.0	0.0	2.5937922573268675	0.0
21	1.5438228790536735E-5	0.0	0.0	2.6747040144180705	0.0
22	1.5438228790536735E-5	0.0	0.0	2.7637208416243055	0.0
23	1.5438228790536735E-5	0.0	0.0	2.868639044484793	0.0
24	1.5438228790536735E-5	0.0	0.0	2.9508321745656105	0.0
25	1.5438228790536735E-5	0.0	0.0	3.0277608686288553	0.0
26	1.5438228790536735E-5	0.0	0.0	3.101061578926324	0.0
27	1.5438228790536735E-5	0.0	0.0	3.180167063249034	0.0
28	1.5438228790536735E-5	0.0	0.0	3.261897046466135	0.0
29	1.5438228790536735E-5	0.0	0.0	3.354480104522984	0.0
30	1.5438228790536735E-5	0.0	0.0	3.480224478021906	0.0
31	1.5438228790536735E-5	0.0	0.0	3.585297063170299	0.0
32	1.5438228790536735E-5	0.0	0.0	3.68743638484849	0.0
33	1.5438228790536735E-5	0.0	0.0	3.794176298706261	0.0
34	1.5438228790536735E-5	0.0	0.0	3.901580056402025	0.0
35	1.5438228790536735E-5	0.0	0.0	4.0239897724821905	0.0
36	1.5438228790536735E-5	0.0	0.0	4.138989138742899	0.0
37	3.087645758107347E-5	0.0	0.0	4.260441684638051	0.0
38	3.087645758107347E-5	0.0	0.0	4.382002298134738	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	54585	0.0	42.690117	1
CGACGGT	420	0.0	38.238094	28
CACGACG	455	0.0	37.23077	26
ACGGGAT	2860	0.0	37.153847	5
AGGGCGA	6050	0.0	37.127274	6
GTTTTTT	62030	0.0	37.091084	2
GGGCGAT	11735	0.0	36.81977	7
TAGGGAT	13005	0.0	36.624374	5
TAGGGCG	2660	0.0	36.473686	5
TACGGGA	2300	0.0	36.25217	4
GGGACCG	4895	0.0	36.13483	7
TAGGGAC	7320	0.0	36.03552	5
ACGGGAC	2180	0.0	36.027523	5
AGGGATT	14865	0.0	35.94887	6
GACCGAT	4170	0.0	35.769783	9
AACGGGA	2410	0.0	35.692947	4
CGTTAGG	960	0.0	35.520836	2
GTAGGGA	9980	0.0	35.513027	4
TGGGCGA	3370	0.0	35.38279	6
TTAGGGA	9860	0.0	35.23124	4
>>END_MODULE