FastQCFastQC Report
Thu 26 May 2016
SRR1547211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547211_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2279793
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93990.412274272269456No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28580.12536225876647575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT254.4442226E-544.021
ACTTACG207.858114E-444.01
CGTTTTT76800.040.87761
GCGATAT2000.039.69
TACGGGA4950.039.5555574
GTAGCAT18900.038.412729
CACGACG2250.038.13333526
CAATTCG357.293358E-637.7142871
ACAACGA1300.037.23076613
AGGGTAC13000.037.0615436
TACGTAG950.037.052631
TAGCATA18650.037.0402230
CGTTAGG3100.036.9032252
TTGATCC19500.036.66666417
TACGCTA301.3014968E-436.66666436
TTCGCGA301.3014968E-436.66666433
TAAGGGA23550.036.433124
GGCGATA10150.036.413798
CATATGC18900.036.20105733
ATAGGGC11250.036.1777764
Produced by FastQC (version 0.11.5)