FastQCFastQC Report
Thu 26 May 2016
SRR1547210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547210_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3001475
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183370.6109329579623352No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31370.10451527998733956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126300.041.108471
TATAGCG1800.039.111111
GCGATAC2750.038.49
CGTCATA6300.037.01587338
AGGGCGA34800.036.7931066
TCGTTGA1500.036.66666824
ACGTTAG601.9826984E-1036.6666681
GTTGATC17100.036.15204616
ACGGGAT9700.036.0618555
TACGGGA9500.035.894744
GGGCGAT67050.035.7643557
CGGTCTA3200.035.7531
GGCGATA17600.035.5000048
TTAGGGA26850.035.2327774
ACCCGCT15200.035.1710534
CATACGA3950.035.08860818
TAAGGGA34700.034.7435154
GCGATAT3550.034.7042249
TCAGACG15800.034.67088322
TTGATCC17850.034.50980417
Produced by FastQC (version 0.11.5)