##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547210_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3001475
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.617084933241156	31.0	30.0	31.0	28.0	33.0
2	30.940105448154657	31.0	31.0	33.0	28.0	34.0
3	31.107509474508365	31.0	31.0	34.0	28.0	34.0
4	34.942703504110476	35.0	35.0	37.0	32.0	37.0
5	34.750438367802495	35.0	35.0	37.0	32.0	37.0
6	34.7721846758677	35.0	35.0	37.0	32.0	37.0
7	35.398709634429736	35.0	35.0	37.0	33.0	37.0
8	34.37051216485228	35.0	35.0	37.0	33.0	37.0
9	36.47833115384936	39.0	37.0	39.0	32.0	39.0
10	35.885921755137055	38.0	35.0	39.0	30.0	39.0
11	35.6809921788454	37.0	35.0	39.0	30.0	39.0
12	35.30205282402819	37.0	34.0	39.0	30.0	39.0
13	35.14593391582472	37.0	34.0	39.0	29.0	39.0
14	36.04810801356	38.0	34.0	40.0	29.0	41.0
15	36.2292636120574	38.0	34.0	40.0	30.0	41.0
16	36.31033908328405	38.0	34.0	40.0	30.0	41.0
17	36.19986206781666	38.0	34.0	40.0	30.0	41.0
18	36.13712458041463	38.0	34.0	40.0	30.0	41.0
19	36.1104650213645	38.0	34.0	40.0	30.0	41.0
20	35.97043153782724	38.0	34.0	40.0	30.0	41.0
21	35.79736729441358	38.0	34.0	40.0	29.0	41.0
22	35.73771595632147	38.0	34.0	40.0	29.0	41.0
23	35.60928010394889	37.0	34.0	40.0	29.0	41.0
24	35.50886014376265	37.0	34.0	40.0	29.0	41.0
25	35.297170557809075	37.0	34.0	40.0	27.0	41.0
26	35.12496156056606	37.0	33.0	40.0	27.0	41.0
27	34.92945168625426	37.0	33.0	40.0	26.0	41.0
28	34.913892336268	37.0	33.0	40.0	26.0	41.0
29	34.73812075729433	37.0	33.0	40.0	26.0	41.0
30	34.756116576016794	37.0	33.0	40.0	26.0	41.0
31	34.709713390916136	37.0	33.0	40.0	25.0	41.0
32	34.50205848791011	37.0	33.0	40.0	25.0	41.0
33	34.374976969656586	37.0	33.0	40.0	24.0	41.0
34	34.22635037773095	37.0	33.0	40.0	24.0	41.0
35	34.031027744692196	36.0	33.0	40.0	23.0	41.0
36	33.97326647731532	37.0	33.0	40.0	23.0	41.0
37	33.98343847608259	37.0	33.0	40.0	23.0	41.0
38	33.96567720870572	36.0	32.0	40.0	23.0	41.0
39	33.85397579523401	36.0	32.0	40.0	23.0	41.0
40	33.754315128395206	36.0	32.0	40.0	23.0	41.0
41	33.74523692517845	36.0	32.0	40.0	23.0	41.0
42	33.582631206323555	36.0	32.0	40.0	22.0	41.0
43	33.61437559866399	36.0	32.0	40.0	23.0	41.0
44	33.30474849865483	35.0	31.0	39.0	21.0	41.0
45	33.22238799257032	35.0	31.0	39.0	22.0	41.0
46	33.15628882466121	35.0	31.0	39.0	21.0	41.0
47	33.07104107147319	35.0	31.0	39.0	21.0	41.0
48	33.059000658009815	35.0	31.0	39.0	21.0	41.0
49	33.05078236533704	35.0	31.0	39.0	21.0	41.0
50	32.4861143271225	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	1.0
11	4.0
12	3.0
13	18.0
14	55.0
15	144.0
16	442.0
17	1143.0
18	2478.0
19	4599.0
20	7955.0
21	12335.0
22	18089.0
23	25251.0
24	34871.0
25	46726.0
26	60381.0
27	73958.0
28	84843.0
29	98774.0
30	116152.0
31	138805.0
32	166424.0
33	202657.0
34	270090.0
35	300130.0
36	282289.0
37	327804.0
38	375535.0
39	349517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.447654903006026	23.65940079460932	27.656635487551956	15.236308814832707
2	32.581847258431274	25.42443298711467	28.22618879051133	13.767530963942729
3	29.169291764882267	26.15683955388601	31.033208672402736	13.640660008828991
4	25.685338042129285	28.425190947784007	30.721162095303146	15.168308914783566
5	22.948616930009415	32.20376648147994	29.71565646890279	15.131960119607859
6	21.231960952531672	41.44472301118617	27.189931616955	10.133384419327164
7	87.91134358940188	3.669329246453827	6.965941745308557	1.4533854188357391
8	86.00138265352868	5.753604477798416	6.58166401519253	1.663348853480372
9	82.82617712957796	5.103557417603012	8.752163519602863	3.3181019332161688
10	40.08589110354076	29.731415387434513	16.012194004614397	14.17049950441033
11	34.1509757702463	25.725518286842302	23.8096602503769	16.313845692534503
12	30.605452319276356	22.670886813983124	27.867032042579066	18.856628824161454
13	24.29468844484795	27.737595682122958	28.879301010336583	19.08841486269251
14	21.101891569978093	30.252892327938763	29.705094994960813	18.94012110712233
15	20.036948500320676	24.144928743367842	36.321941711991606	19.496181044319876
16	23.242405817139904	22.078211545989888	34.73835364279229	19.94102899407791
17	22.62800789611774	22.230136849382387	29.15323299377806	25.98862226072181
18	24.045077836730275	23.418086107663733	31.123331028910783	21.41350502669521
19	27.9473925320051	24.78941187249602	26.776368285592916	20.486827309905962
20	29.551104040513415	24.66933757569195	25.620969689902463	20.15858869389217
21	26.745983224914415	25.731981775626984	27.909477840061967	19.61255715939663
22	25.477873378922027	23.296146061519753	27.58090605452319	23.645074505035023
23	23.774744084158623	26.68414696107747	26.926094670120527	22.615014284643383
24	23.330595790403052	24.550795858702802	31.68065701030327	20.437951340590878
25	23.284618395955324	25.184417661316523	29.84286059354151	21.688103349186648
26	21.68970256290657	27.762616713449223	27.625417503060994	22.922263220583215
27	22.12335601662516	26.701505093329114	28.522543082984196	22.65259580706153
28	21.058746116492724	26.74978135749923	31.30310930459191	20.888363221416135
29	22.188024221424467	25.02436302151442	31.13625800648015	21.651354750580964
30	23.088314911835013	25.813008604102983	30.108430021905896	20.990246462156108
31	26.517962002015675	25.372525175122227	27.10523992370418	21.004272899157915
32	26.797990987764347	25.06564272565988	27.46856129069874	20.667804995877027
33	26.459257531713575	24.74593324948567	26.707935265161296	22.08687395363946
34	22.329288100017493	25.97129744542267	28.803704845117817	22.895709609442026
35	22.60212062402652	25.585054015109236	29.543574409248784	22.269250951615454
36	26.885647889787524	25.694400253208837	26.305499795933667	21.114452061069976
37	23.482887580273033	28.051807861134943	26.943352851514675	21.521951707077353
38	23.747757352634956	29.02089805845459	25.574226005547274	21.65711858336318
39	23.40006163636212	27.239607193130045	25.967266094170366	23.393065076337468
40	25.07193962968207	24.731773544673867	27.30757377622669	22.88871304941737
41	21.793651454701436	25.165293730582462	27.739694650130353	25.301360164585745
42	23.21598547380871	26.582563572909983	25.690202317194043	24.511248636087256
43	22.84430155173706	25.502494606818317	27.149684738336983	24.50351910310764
44	22.684846617079934	26.362638369468343	28.1437959669829	22.80871904646882
45	21.903031009753537	27.99610191655769	26.803621552736573	23.2972455209522
46	23.264428322741317	27.526799323665863	27.189098693142537	22.01967366045028
47	22.539384802472117	26.8416028785847	27.828550962443465	22.790461356499723
48	23.222182426973404	25.701363496280994	28.756761259047636	22.319692817697963
49	23.240606701704994	25.312388075862703	28.94610150009579	22.500903722336517
50	22.112794542683183	27.83304875103074	27.548488659742294	22.505668046543782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2021.0
1	3058.0
2	4095.0
3	17932.0
4	31769.0
5	22950.0
6	14131.0
7	14282.0
8	14433.0
9	14697.0
10	14961.0
11	15145.5
12	15330.0
13	15389.0
14	15448.0
15	14809.0
16	14170.0
17	13614.0
18	13058.0
19	12776.5
20	12495.0
21	13034.5
22	13574.0
23	14577.5
24	15581.0
25	19055.0
26	22529.0
27	25061.5
28	27594.0
29	32530.0
30	37466.0
31	45510.0
32	53554.0
33	60069.5
34	66585.0
35	73248.5
36	79912.0
37	88260.0
38	96608.0
39	112215.0
40	127822.0
41	146595.0
42	165368.0
43	182186.5
44	199005.0
45	205126.5
46	211248.0
47	216106.0
48	220964.0
49	225555.5
50	230147.0
51	215020.5
52	199894.0
53	189027.5
54	178161.0
55	168961.0
56	159761.0
57	156676.0
58	153591.0
59	147827.0
60	142063.0
61	131798.0
62	121533.0
63	109721.5
64	97910.0
65	84714.0
66	71518.0
67	60260.0
68	49002.0
69	43586.5
70	38171.0
71	33136.5
72	28102.0
73	23141.5
74	18181.0
75	14392.0
76	10603.0
77	8639.0
78	6675.0
79	4832.5
80	2990.0
81	2495.0
82	2000.0
83	1441.5
84	883.0
85	597.0
86	311.0
87	248.5
88	186.0
89	115.0
90	44.0
91	31.5
92	19.0
93	13.0
94	7.0
95	4.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3001475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.99176173196225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.17751146606722	17.852364901553834
2	5.856783816753689	2.5760198842731916
3	1.8574672722230992	1.2254693302694475
4	1.077724047498893	0.9480420186160647
5	0.743897424843935	0.8179807460094057
6	0.5582463822392759	0.7366092855561648
7	0.45677092044730655	0.7031638073996376
8	0.37778135259952067	0.6646461994517661
9	0.32891912676512464	0.6510159958413336
>10	5.029881335372407	27.856946018898682
>50	2.1109708364509427	32.6496928875138
>100	0.4197004325626373	11.888945400728614
>500	0.003259189631977288	0.4686542114233558
>1k	9.311970377077964E-4	0.3345872285036164
>5k	0.0	0.0
>10k+	1.5519950628463275E-4	0.6258620839611153
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18337	0.6109329579623352	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3137	0.10451527998733956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016092088056705454	0.0
2	0.0	0.0	0.0	0.07169808177645991	0.0
3	0.0	0.0	0.0	0.12267301909894301	0.0
4	0.0	0.0	0.0	0.18767439342323358	0.0
5	0.0	0.0	0.0	0.30601620869739043	0.0
6	0.0	0.0	0.0	0.567554285809477	0.0
7	0.0	0.0	0.0	0.6940920713982291	0.0
8	0.0	0.0	0.0	1.0091371742226738	0.0
9	0.0	0.0	0.0	1.181185916924179	0.0
10	0.0	0.0	0.0	1.42663190597956	0.0
11	0.0	0.0	0.0	1.6019123930734056	0.0
12	0.0	0.0	0.0	1.7588352393406574	0.0
13	0.0	0.0	0.0	1.8444264903089314	0.0
14	0.0	0.0	0.0	1.8884381845592584	0.0
15	0.0	0.0	0.0	1.9313504193771396	0.0
16	0.0	0.0	0.0	2.0090455526033035	0.0
17	0.0	0.0	0.0	2.0860077128745034	0.0
18	0.0	0.0	0.0	2.2150775868531305	0.0
19	0.0	0.0	0.0	2.273882008012727	0.0
20	0.0	0.0	0.0	2.34004947567446	0.0
21	0.0	0.0	0.0	2.4125804812633787	0.0
22	0.0	0.0	0.0	2.493240823261896	0.0
23	0.0	0.0	0.0	2.5796983149951274	0.0
24	0.0	0.0	0.0	2.652462539251535	0.0
25	0.0	0.0	0.0	2.716697623668363	0.0
26	0.0	0.0	0.0	2.7789670078877884	0.0
27	0.0	0.0	0.0	2.8491658268018223	0.0
28	0.0	0.0	0.0	2.920963859435777	0.0
29	0.0	0.0	0.0	2.9964933907495483	3.3316952498354974E-5
30	0.0	0.0	0.0	3.0994094570169666	3.3316952498354974E-5
31	0.0	0.0	0.0	3.1860335335126897	3.3316952498354974E-5
32	0.0	0.0	0.0	3.284984882432804	3.3316952498354974E-5
33	0.0	0.0	0.0	3.375407091513339	3.3316952498354974E-5
34	0.0	0.0	0.0	3.4654294971638944	3.3316952498354974E-5
35	0.0	0.0	0.0	3.566746349711392	3.3316952498354974E-5
36	0.0	0.0	0.0	3.6559358315494883	3.3316952498354974E-5
37	0.0	0.0	0.0	3.7533212837021797	3.3316952498354974E-5
38	0.0	0.0	0.0	3.861934548846817	3.3316952498354974E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12630	0.0	41.10847	1
TATAGCG	180	0.0	39.11111	1
GCGATAC	275	0.0	38.4	9
CGTCATA	630	0.0	37.015873	38
AGGGCGA	3480	0.0	36.793106	6
TCGTTGA	150	0.0	36.666668	24
ACGTTAG	60	1.9826984E-10	36.666668	1
GTTGATC	1710	0.0	36.152046	16
ACGGGAT	970	0.0	36.061855	5
TACGGGA	950	0.0	35.89474	4
GGGCGAT	6705	0.0	35.764355	7
CGGTCTA	320	0.0	35.75	31
GGCGATA	1760	0.0	35.500004	8
TTAGGGA	2685	0.0	35.232777	4
ACCCGCT	1520	0.0	35.17105	34
CATACGA	395	0.0	35.088608	18
TAAGGGA	3470	0.0	34.743515	4
GCGATAT	355	0.0	34.704224	9
TCAGACG	1580	0.0	34.670883	22
TTGATCC	1785	0.0	34.509804	17
>>END_MODULE