FastQCFastQC Report
Thu 26 May 2016
SRR1547209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547209_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2659163
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137540.5172304217530103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA600.044.014
CGTTTTT102950.041.350171
CGACGGT2750.040.028
CGAGTTA900.039.1111137
TACGGGA9000.038.1333354
ACGTTCG750.038.1333313
GCGCGAC3550.037.802829
CTATCGT357.2938165E-637.71428732
CGGTCTA3100.037.61290431
AGGGCGA24550.037.458256
TACGCTA651.0913936E-1137.2307721
TAGGGCG10000.036.965
TAGGGAT54000.036.788895
CGGGATA6600.036.6666686
GCGATCG900.036.6666689
AGGGATG51700.036.4680866
GGGCGAT51200.036.394537
AGGGATT64250.036.261486
CGACGTC850.036.23529418
GACGGTC3050.036.06557529
Produced by FastQC (version 0.11.5)