##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547209_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2659163
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.59940176664612	31.0	30.0	31.0	28.0	33.0
2	30.920410294517485	31.0	31.0	33.0	28.0	34.0
3	31.09096057669274	31.0	31.0	34.0	28.0	34.0
4	34.91853714871935	35.0	35.0	37.0	32.0	37.0
5	34.70774450456779	35.0	35.0	37.0	32.0	37.0
6	34.75041356998424	35.0	35.0	37.0	32.0	37.0
7	35.44076350340314	35.0	35.0	37.0	35.0	37.0
8	34.423767177867624	36.0	35.0	37.0	33.0	37.0
9	36.52655704069288	39.0	37.0	39.0	32.0	39.0
10	35.921176325031595	38.0	35.0	39.0	30.0	39.0
11	35.57815861607581	37.0	35.0	39.0	30.0	39.0
12	35.3395233763406	37.0	34.0	39.0	30.0	39.0
13	35.21461602767487	37.0	34.0	39.0	30.0	39.0
14	36.148964918660496	38.0	34.0	40.0	29.0	41.0
15	36.34115208432127	38.0	34.0	40.0	30.0	41.0
16	36.40405721650008	38.0	35.0	40.0	30.0	41.0
17	36.2991151727066	38.0	34.0	40.0	30.0	41.0
18	36.21590590723472	38.0	34.0	40.0	30.0	41.0
19	36.22208980795837	38.0	34.0	40.0	30.0	41.0
20	36.09391865034223	38.0	34.0	40.0	30.0	41.0
21	35.925445337499056	38.0	34.0	40.0	29.0	41.0
22	35.88229341337857	38.0	34.0	40.0	29.0	41.0
23	35.75772000437732	38.0	34.0	40.0	29.0	41.0
24	35.68857381063139	38.0	34.0	40.0	29.0	41.0
25	35.49437398158744	37.0	34.0	40.0	28.0	41.0
26	35.32909791539669	37.0	34.0	40.0	27.0	41.0
27	35.11805481649677	37.0	33.0	40.0	27.0	41.0
28	35.16298060705568	37.0	33.0	40.0	27.0	41.0
29	35.026896809259156	37.0	33.0	40.0	27.0	41.0
30	35.03616589129737	37.0	33.0	40.0	27.0	41.0
31	34.99751575965821	37.0	33.0	40.0	26.0	41.0
32	34.77669289171066	37.0	33.0	40.0	25.0	41.0
33	34.627042042928544	37.0	33.0	40.0	25.0	41.0
34	34.545833783036244	37.0	33.0	40.0	24.0	41.0
35	34.36811244741296	37.0	33.0	40.0	24.0	41.0
36	34.331995443679084	37.0	33.0	40.0	24.0	41.0
37	34.35580744768185	37.0	33.0	40.0	24.0	41.0
38	34.37462878356836	37.0	33.0	40.0	24.0	41.0
39	34.27599511575635	37.0	33.0	40.0	23.0	41.0
40	34.20292099431287	37.0	33.0	40.0	23.0	41.0
41	34.227607333585794	37.0	33.0	40.0	23.0	41.0
42	34.074587379562665	37.0	33.0	40.0	23.0	41.0
43	34.14041147534017	37.0	33.0	40.0	23.0	41.0
44	33.747895484406186	36.0	32.0	40.0	23.0	41.0
45	33.71103275729995	36.0	32.0	40.0	23.0	41.0
46	33.68382758033261	36.0	32.0	40.0	23.0	41.0
47	33.64379656305386	36.0	32.0	40.0	23.0	41.0
48	33.67219760503587	36.0	32.0	40.0	23.0	41.0
49	33.688212794777904	36.0	32.0	40.0	23.0	41.0
50	33.11647048338143	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	9.0
13	15.0
14	32.0
15	117.0
16	376.0
17	909.0
18	2027.0
19	3755.0
20	6494.0
21	9896.0
22	14335.0
23	20501.0
24	28156.0
25	38225.0
26	49986.0
27	60846.0
28	70661.0
29	82146.0
30	98029.0
31	118667.0
32	141932.0
33	172520.0
34	230303.0
35	260101.0
36	244737.0
37	288123.0
38	352979.0
39	363283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.466996946031514	24.98823878039819	26.89086753989883	14.65389673367146
2	31.10268155806921	26.184705488155487	28.917219440854137	13.795393512921173
3	27.602369617808307	26.68963880739917	31.86581642419062	13.842175150601898
4	24.54411406897584	29.379169310042297	31.25126214526902	14.825454475712846
5	22.088115696555647	33.58135623878641	30.362937510788168	13.96759055386977
6	20.812902405757	41.77494948598488	27.327847145887635	10.08430096237049
7	89.96823436547515	2.86473600903743	6.003994489995536	1.1630351354918822
8	88.19542088995672	5.066218204750894	5.555281868768481	1.1830790365238988
9	84.9510165416712	5.5815307297822665	7.240511393998789	2.2269413345477505
10	38.63561579339063	38.24199569563807	12.825163406680975	10.297225104290336
11	28.065522873174753	25.316800812887365	29.989248496613406	16.62842781732447
12	27.69164582990964	23.63627201491597	31.023145252848362	17.64893690232603
13	21.808290804286912	29.809417474596327	31.20387881449915	17.17841290661761
14	18.36920113584613	32.30125419163849	30.91149357899459	18.41805109352078
15	17.805828375319603	26.4756993083914	38.34778086187271	17.370691454416296
16	20.70399595662244	24.426219829322235	36.8162463150999	18.053537898955422
17	20.607950697268276	24.04425753517178	30.59744739228095	24.75034437527899
18	22.006661494613155	25.341808681904794	33.23538271253022	19.41614711095183
19	25.79567330020762	25.972270221870563	28.847648677422182	19.38440780049963
20	26.695430103382158	26.084749223721897	28.351251878880685	18.868568794015257
21	23.7616122065477	27.826312264423052	29.87018847659959	18.541887052429658
22	23.526312602875414	25.159232435168512	29.5183108369062	21.796144125049874
23	20.26893424735528	28.771609713281958	29.817916389480448	21.14153964988231
24	20.361971041263736	26.541095826017436	34.446628506789544	18.650304625929287
25	20.38566270664867	27.27895958239491	32.31998188903802	20.0153958219184
26	20.30018468217255	30.328941851251688	29.053991801179546	20.316881665396217
27	19.641518778653282	29.720968590492575	30.493918575130596	20.14359405572355
28	18.313958189099353	28.483699570127897	34.012883001154876	19.18945923961788
29	19.28539168151783	26.714383435690102	33.58594414859112	20.414280734200947
30	20.53947050256039	28.107114907961638	31.75198361288872	19.601430976589253
31	24.216228941211952	27.682394798664088	28.551126801929783	19.550249458194177
32	25.080072188128373	27.252936356289553	28.726369914142158	18.94062154143992
33	23.87849108911338	27.68694510265072	28.737538842109338	19.69702496612656
34	20.85174921582468	27.698978964433547	30.313485860024375	21.1357859597174
35	20.45805390643597	27.781598946736246	30.78337055682559	20.97697659000219
36	24.18388793767061	28.221624623988827	28.356366270138384	19.238121168202174
37	21.183846195212556	31.277962276099657	28.17999498338387	19.35819654530392
38	20.70166439590202	31.232948111868286	27.910398873630538	20.15498861859916
39	21.030564880753833	30.56345173274448	27.995425628289805	20.41055775821189
40	22.660965123236146	27.86252666722574	28.658228171796914	20.8182800377412
41	20.102678925661944	27.54374214743511	29.130858093317336	23.222720833585605
42	21.209380545682986	28.53631010960968	27.376321045381573	22.877988299325764
43	21.091411094393237	27.826650716785693	28.75833485950278	22.32360332931829
44	20.686321222128917	29.35675624247179	29.219382189057235	20.737540346342062
45	20.109034308916	30.431229676405696	27.988092493765897	21.471643520912405
46	20.669248180724537	29.891210128901463	29.098855542138637	20.340686148235363
47	21.18147702867406	28.37836567371011	28.922446649566048	21.517710648049782
48	21.322235605715033	26.88515145555199	31.120281080926592	20.672331857806384
49	21.03654420582717	27.224355934555344	30.715266420298416	21.023833439319066
50	19.963386975525758	30.300399035335555	29.420723739011112	20.315490250127578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1444.0
1	2240.5
2	3037.0
3	13942.5
4	24848.0
5	18476.5
6	12105.0
7	12067.5
8	12030.0
9	12458.5
10	12887.0
11	13040.0
12	13193.0
13	12818.5
14	12444.0
15	12568.5
16	12693.0
17	12263.0
18	11833.0
19	12100.5
20	12368.0
21	13973.0
22	15578.0
23	17931.5
24	20285.0
25	25537.5
26	30790.0
27	36271.5
28	41753.0
29	48537.0
30	55321.0
31	62291.0
32	69261.0
33	79935.5
34	90610.0
35	101319.0
36	112028.0
37	123783.5
38	135539.0
39	147522.0
40	159505.0
41	177430.0
42	195355.0
43	205591.0
44	215827.0
45	220311.5
46	224796.0
47	212982.0
48	201168.0
49	196003.5
50	190839.0
51	178006.5
52	165174.0
53	151266.5
54	137359.0
55	125806.5
56	114254.0
57	105831.5
58	97409.0
59	86675.0
60	75941.0
61	68720.5
62	61500.0
63	53281.0
64	45062.0
65	36038.0
66	27014.0
67	22112.0
68	17210.0
69	14757.0
70	12304.0
71	9940.0
72	7576.0
73	5990.5
74	4405.0
75	3690.0
76	2975.0
77	2254.5
78	1534.0
79	1300.0
80	1066.0
81	797.0
82	528.0
83	379.0
84	230.0
85	141.5
86	53.0
87	31.0
88	9.0
89	7.0
90	5.0
91	5.5
92	6.0
93	5.5
94	5.0
95	6.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2659163.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.179201573439336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57767908026993	17.277501091343815
2	5.767067066070161	2.442837517596866
3	1.8481279352719395	1.1742562222388595
4	0.9971660728886043	0.8447672503961059
5	0.659883012518055	0.6987897668504138
6	0.5013045399516348	0.6370337940789567
7	0.42840763257999326	0.6351332124208129
8	0.374652562693177	0.6347873716227531
9	0.3059919275457529	0.583259824200307
>10	4.784692882493536	25.447543337602525
>50	2.1463267777717716	32.52410116701855
>100	0.6074251800830064	16.358359159756404
>500	9.109499018226024E-4	0.1296888338949061
>1k	1.8218998036452047E-4	0.08122433661076386
>5k	0.0	0.0
>10k+	1.8218998036452047E-4	0.5307171143679079
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13754	0.5172304217530103	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012597949053894025	0.0
2	0.0	0.0	0.0	0.05490449438413516	0.0
3	0.0	0.0	0.0	0.09299918809038785	0.0
4	0.0	0.0	0.0	0.14369183085053455	0.0
5	0.0	0.0	0.0	0.23082451132179563	0.0
6	0.0	0.0	0.0	0.4123477951520836	0.0
7	0.0	0.0	0.0	0.5048205017894728	0.0
8	0.0	0.0	0.0	0.7853222987834894	0.0
9	0.0	0.0	0.0	0.923937344194395	0.0
10	0.0	0.0	0.0	1.1238498730615611	0.0
11	0.0	0.0	0.0	1.2618256195652542	0.0
12	0.0	0.0	0.0	1.3902118824607592	0.0
13	0.0	0.0	0.0	1.4547810720892251	0.0
14	0.0	0.0	0.0	1.4849409381824281	0.0
15	0.0	0.0	0.0	1.5172443359056966	0.0
16	0.0	0.0	0.0	1.582114372078733	0.0
17	0.0	0.0	0.0	1.6598079922140914	0.0
18	0.0	0.0	0.0	1.7692033169835772	0.0
19	0.0	0.0	0.0	1.8204224411967225	0.0
20	3.7605818071325454E-5	0.0	0.0	1.8796516046590601	0.0
21	3.7605818071325454E-5	0.0	0.0	1.9471916539151606	0.0
22	3.7605818071325454E-5	0.0	0.0	2.027216834770941	0.0
23	3.7605818071325454E-5	0.0	0.0	2.1192006657734033	0.0
24	3.7605818071325454E-5	0.0	0.0	2.1901628444739942	0.0
25	3.7605818071325454E-5	0.0	0.0	2.2540551293771762	0.0
26	3.7605818071325454E-5	0.0	0.0	2.31516458374308	0.0
27	3.7605818071325454E-5	0.0	0.0	2.3844345006304617	0.0
28	3.7605818071325454E-5	0.0	0.0	2.451485674251635	0.0
29	3.7605818071325454E-5	0.0	0.0	2.528201543117139	0.0
30	3.7605818071325454E-5	0.0	0.0	2.625863852648371	0.0
31	3.7605818071325454E-5	0.0	0.0	2.711266665488351	0.0
32	3.7605818071325454E-5	0.0	0.0	2.7963686317837606	0.0
33	3.7605818071325454E-5	0.0	0.0	2.8841782169803056	0.0
34	3.7605818071325454E-5	0.0	0.0	2.9766133177996235	0.0
35	3.7605818071325454E-5	0.0	0.0	3.07950283604277	0.0
36	3.7605818071325454E-5	0.0	0.0	3.1740062568560106	0.0
37	3.7605818071325454E-5	0.0	0.0	3.2697882754836765	0.0
38	3.7605818071325454E-5	0.0	0.0	3.3738435740870343	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	60	0.0	44.0	14
CGTTTTT	10295	0.0	41.35017	1
CGACGGT	275	0.0	40.0	28
CGAGTTA	90	0.0	39.11111	37
TACGGGA	900	0.0	38.133335	4
ACGTTCG	75	0.0	38.13333	13
GCGCGAC	355	0.0	37.80282	9
CTATCGT	35	7.2938165E-6	37.714287	32
CGGTCTA	310	0.0	37.612904	31
AGGGCGA	2455	0.0	37.45825	6
TACGCTA	65	1.0913936E-11	37.23077	21
TAGGGCG	1000	0.0	36.96	5
TAGGGAT	5400	0.0	36.78889	5
CGGGATA	660	0.0	36.666668	6
GCGATCG	90	0.0	36.666668	9
AGGGATG	5170	0.0	36.468086	6
GGGCGAT	5120	0.0	36.39453	7
AGGGATT	6425	0.0	36.26148	6
CGACGTC	85	0.0	36.235294	18
GACGGTC	305	0.0	36.065575	29
>>END_MODULE