FastQCFastQC Report
Thu 26 May 2016
SRR1547208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547208_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3871555
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472631.220775631496905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT306350.042.700181
GTTTTTT348850.037.5548252
TAATGCG1700.037.529411
GGGCGAT82850.036.3789987
GCGCGAC18300.035.9453549
ATTGGGA28200.035.4964524
TATGGGA29600.035.4527054
ACGATAG1800.035.4444471
GTTGATC22550.035.41463516
ACGGGTA3300.035.3333325
TAGGGAT37050.035.2712555
TAAGGGA36900.035.1761554
AGGGCGA43950.035.0898746
GGCGATA19000.035.084218
ATAGGGA41100.034.7931864
ATAGGGC22650.034.7726254
TAGGGAC29700.034.740745
GCCAGTA22050.034.7210925
CGTAAGG7750.034.632262
TACGGGT3050.034.622954
Produced by FastQC (version 0.11.5)