##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547208_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3871555
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.607825279506557	31.0	30.0	31.0	28.0	34.0
2	30.94391426700641	31.0	31.0	33.0	28.0	34.0
3	31.06326243589462	31.0	31.0	34.0	28.0	34.0
4	34.9142440182304	35.0	35.0	37.0	32.0	37.0
5	34.73306436302726	35.0	35.0	37.0	32.0	37.0
6	34.7624019289407	35.0	35.0	37.0	32.0	37.0
7	35.388377538224304	35.0	35.0	37.0	33.0	37.0
8	34.35068028221219	35.0	35.0	37.0	32.0	37.0
9	36.4582169180084	39.0	37.0	39.0	32.0	39.0
10	35.90022303699676	38.0	35.0	39.0	30.0	39.0
11	35.70693274407829	37.0	35.0	39.0	30.0	39.0
12	35.390292014448974	37.0	35.0	39.0	30.0	39.0
13	35.299856001012515	37.0	34.0	39.0	30.0	39.0
14	36.17870881338377	38.0	34.0	40.0	29.0	41.0
15	36.30716004292849	38.0	34.0	40.0	30.0	41.0
16	36.349864589292935	38.0	34.0	40.0	30.0	41.0
17	36.2460628868762	38.0	34.0	40.0	30.0	41.0
18	36.110804831650334	38.0	34.0	40.0	30.0	41.0
19	36.01133343062413	37.0	34.0	40.0	30.0	41.0
20	35.819471246049716	37.0	34.0	40.0	29.0	41.0
21	35.652455925332326	37.0	34.0	40.0	29.0	41.0
22	35.62401644817134	37.0	34.0	40.0	29.0	41.0
23	35.47840957961336	37.0	34.0	40.0	29.0	41.0
24	35.34116756703702	37.0	34.0	40.0	27.0	41.0
25	35.12609997791585	37.0	33.0	40.0	27.0	41.0
26	34.94372519569011	37.0	33.0	40.0	27.0	41.0
27	34.724082958914444	36.0	33.0	40.0	26.0	41.0
28	34.7030459337398	36.0	33.0	40.0	25.0	41.0
29	34.459396030793826	36.0	33.0	40.0	25.0	41.0
30	34.43366993365715	36.0	33.0	40.0	25.0	41.0
31	34.33156987308717	36.0	33.0	40.0	24.0	41.0
32	34.00726710585282	36.0	33.0	40.0	23.0	41.0
33	33.72618185716075	36.0	32.0	40.0	22.0	41.0
34	33.45343744309457	36.0	32.0	40.0	21.0	41.0
35	33.15747961736305	36.0	31.0	40.0	19.0	41.0
36	33.08184024248655	36.0	31.0	40.0	18.0	41.0
37	33.09001835179921	36.0	31.0	40.0	18.0	41.0
38	33.063205094593776	36.0	31.0	40.0	18.0	41.0
39	32.92752266208281	36.0	31.0	40.0	18.0	41.0
40	32.81889189227584	35.0	31.0	40.0	18.0	41.0
41	32.80170344990579	35.0	31.0	40.0	18.0	41.0
42	32.63052003652279	35.0	31.0	39.0	18.0	41.0
43	32.64944938145009	35.0	31.0	39.0	18.0	41.0
44	32.336945490894486	35.0	30.0	39.0	16.0	41.0
45	32.25127913719423	35.0	30.0	39.0	17.0	41.0
46	32.18798338135452	35.0	30.0	39.0	17.0	41.0
47	32.105715145464806	35.0	30.0	39.0	17.0	40.0
48	32.073452914914036	35.0	30.0	39.0	17.0	40.0
49	32.073549000337074	35.0	30.0	39.0	17.0	40.0
50	31.53348460760599	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	4.0
12	12.0
13	20.0
14	89.0
15	264.0
16	702.0
17	1926.0
18	4090.0
19	8106.0
20	13401.0
21	20359.0
22	29484.0
23	40829.0
24	56042.0
25	76191.0
26	100482.0
27	118792.0
28	127457.0
29	138013.0
30	155125.0
31	179430.0
32	212690.0
33	255210.0
34	330684.0
35	365904.0
36	361496.0
37	407562.0
38	458754.0
39	408435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.95979393292876	23.05833185890424	25.87717338382123	19.104700824345773
2	35.85600617839602	24.2537946639012	26.73809360838216	13.152105549320622
3	28.116583646622612	24.61233277068258	33.804530737649344	13.466552845045465
4	25.165082247314064	27.150021115546597	33.05126751395757	14.633629123181771
5	22.186924891936187	30.721221834637504	32.76290792717655	14.328945346249764
6	20.438970904455704	39.54028807546322	29.958840827522792	10.061900192558287
7	84.1275921432086	3.344986704308734	11.043237148897536	1.4841840035851228
8	81.8677249838889	5.645819315494679	10.639084295586656	1.8473714050297618
9	78.90754490120895	4.993084174188407	12.877590528870183	3.221780395732464
10	38.140152987623836	28.407991104349545	19.431313774439467	14.02054213358715
11	31.9726569814971	24.511933835371057	27.030637560360116	16.484771622771728
12	28.719519676202452	20.92443475554396	31.490654271991485	18.865391296262096
13	24.281302990658794	23.90778382329581	32.80826437955808	19.002648806487315
14	21.174386002523534	25.968377047465424	33.55411456120345	19.303122388807598
15	20.245172805242337	22.335805638819544	37.62622512143054	19.792796434507583
16	23.79749222211747	20.978082450074968	35.338358876472114	19.886066451335445
17	23.240455062629874	21.187068245188303	32.130113094092685	23.442363598089138
18	24.649165516181483	21.626607396769515	33.113593891859985	20.610633195189013
19	27.083303737128876	23.50680798800482	29.23109706564933	20.178791209216968
20	28.252937127329975	23.532430767482314	28.491704237702937	19.72292786748477
21	26.647974780159394	23.93170186139678	30.271531723041516	19.14879163540231
22	25.18404103777423	21.75500541771975	30.251823879552276	22.809129664953744
23	23.903986899320813	24.402701240199352	30.150081814671363	21.543230045808468
24	23.68983522124831	23.175571572662665	32.73725931828426	20.397333887804773
25	24.479853702194596	23.210182988489123	30.89652607285703	21.413437236459252
26	22.177858767342837	24.69663998057628	29.96640884605798	23.159092406022904
27	22.399578463950533	23.939708979983497	31.412081192182466	22.248631363883504
28	21.881027132508773	24.861302499899914	32.09798130208663	21.15968906550469
29	23.113426000663818	24.21448229458189	31.69796115514309	20.974130549611203
30	23.811698400255192	23.67785037278303	32.05017105529949	20.46028017166229
31	26.00497732823116	23.701975046202367	29.640286654845404	20.65276097072107
32	26.880258707418598	23.648379010500946	28.899602356159214	20.57175992592124
33	26.527893830773426	24.00689128786754	28.19360696154388	21.271607919815164
34	23.78114220255169	25.394783233093683	29.75409105643598	21.069983507918653
35	23.49575299847219	25.23562237912157	29.833542336348057	21.435082286058186
36	26.020784930086233	26.919312782589945	26.573224453740163	20.486677833583663
37	23.79904198700522	27.788343443396773	27.004859804393845	21.407754765204164
38	24.551788622401077	27.572771147510494	26.68248287832667	21.19295735176176
39	23.734081008793627	26.396292962388497	26.503433374961737	23.36619265385614
40	25.206383481572647	24.35819715850608	28.283829107425827	22.151590252495446
41	22.348229587336355	24.87442384261621	28.252058927226916	24.52528764282052
42	23.535297832524655	26.138334596822205	26.85876863430844	23.4675989363447
43	23.027956467104303	25.02348539540314	28.14770809145163	23.80085004604093
44	23.57024503074346	25.116419629838653	28.639680955068442	22.67365438434944
45	22.86605769516383	26.389990585178307	27.341339590939555	23.402612128718307
46	23.57014171308428	26.18061734884304	27.793328520452377	22.45591241762031
47	22.880444679205127	26.440900361740955	28.135568266497568	22.54308669255635
48	22.739312756760526	25.831661954950917	29.304891703721115	22.12413358456744
49	23.708354911656944	25.02568089566079	28.905181509755124	22.360782682927145
50	22.254494641042164	26.15894646982931	28.893816567244947	22.692742321883586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2238.0
1	4051.0
2	5864.0
3	43027.0
4	80190.0
5	57129.0
6	34068.0
7	34016.5
8	33965.0
9	34423.5
10	34882.0
11	34276.5
12	33671.0
13	32528.0
14	31385.0
15	29730.5
16	28076.0
17	26597.0
18	25118.0
19	24042.5
20	22967.0
21	22391.5
22	21816.0
23	22375.5
24	22935.0
25	23934.5
26	24934.0
27	29204.0
28	33474.0
29	38585.0
30	43696.0
31	49192.0
32	54688.0
33	62542.0
34	70396.0
35	82308.0
36	94220.0
37	103769.5
38	113319.0
39	124749.0
40	136179.0
41	157893.5
42	179608.0
43	197607.0
44	215606.0
45	230969.5
46	246333.0
47	248187.5
48	250042.0
49	252510.0
50	254978.0
51	243920.5
52	232863.0
53	227137.5
54	221412.0
55	217603.5
56	213795.0
57	218821.0
58	223847.0
59	214727.0
60	205607.0
61	196125.0
62	186643.0
63	166566.5
64	146490.0
65	128240.5
66	109991.0
67	93293.0
68	76595.0
69	67407.0
70	58219.0
71	49795.0
72	41371.0
73	33889.0
74	26407.0
75	21115.5
76	15824.0
77	12514.0
78	9204.0
79	6840.0
80	4476.0
81	3389.5
82	2303.0
83	1654.0
84	1005.0
85	743.5
86	482.0
87	360.5
88	239.0
89	158.0
90	77.0
91	60.5
92	44.0
93	26.0
94	8.0
95	6.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3871555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.18065352658762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30062339141652	20.22022175588257
2	5.571527780931927	2.805894213308089
3	2.0998761694904444	1.5862876281803062
4	1.2257902381903076	1.2346479713657381
5	0.8424190983559185	1.0606331719940363
6	0.6609709522377705	0.9986208323662797
7	0.5410554005861116	0.9536890006593566
8	0.44066255541484617	0.8876936904033569
9	0.3798132683334072	0.8607551683233942
>10	6.50386018486282	40.716635963690514
>50	1.298236107535372	21.615600548249855
>100	0.13046418200830492	4.70665195830605
>500	0.0031337804241586137	0.5548996140686632
>1k	0.001462430864547674	0.5545839714958504
>5k	0.0	0.0
>10k+	1.04459347467691E-4	1.243184511705956
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47263	1.220775631496905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013689589841807749	0.0
2	0.0	0.0	0.0	0.0653484194335351	0.0
3	0.0	0.0	0.0	0.12573759122626438	0.0
4	0.0	0.0	0.0	0.1943405169240783	0.0
5	0.0	2.582941479586368E-5	0.0	0.321911996600849	0.0
6	0.0	2.582941479586368E-5	0.0	0.6389938926348715	0.0
7	0.0	2.582941479586368E-5	0.0	0.8275744500594723	0.0
8	0.0	2.582941479586368E-5	0.0	1.22286781409537	0.0
9	0.0	2.582941479586368E-5	0.0	1.4848555683698152	0.0
10	0.0	2.582941479586368E-5	0.0	1.8124242068109584	0.0
11	0.0	2.582941479586368E-5	0.0	2.0048016882105513	0.0
12	0.0	2.582941479586368E-5	0.0	2.1657447718035776	0.0
13	0.0	2.582941479586368E-5	0.0	2.2642839892497975	0.0
14	0.0	2.582941479586368E-5	0.0	2.3239757668430383	0.0
15	0.0	2.582941479586368E-5	0.0	2.3679374308255987	0.0
16	0.0	2.582941479586368E-5	0.0	2.4292822909657747	0.0
17	0.0	2.582941479586368E-5	0.0	2.492564357215641	0.0
18	0.0	2.582941479586368E-5	0.0	2.5979483695827645	0.0
19	0.0	2.582941479586368E-5	0.0	2.647902457797965	0.0
20	0.0	2.582941479586368E-5	0.0	2.7082916295906942	0.0
21	0.0	2.582941479586368E-5	0.0	2.7702305662711755	0.0
22	0.0	2.582941479586368E-5	0.0	2.8409256745674543	0.0
23	0.0	2.582941479586368E-5	0.0	2.918465577784637	0.0
24	0.0	2.582941479586368E-5	0.0	2.9849246620543943	0.0
25	0.0	2.582941479586368E-5	0.0	3.045417151506307	0.0
26	0.0	2.582941479586368E-5	0.0	3.104334046655672	0.0
27	0.0	2.582941479586368E-5	0.0	3.1726528487907313	0.0
28	2.582941479586368E-5	2.582941479586368E-5	0.0	3.244484451338028	0.0
29	2.582941479586368E-5	2.582941479586368E-5	0.0	3.3198288542975627	0.0
30	2.582941479586368E-5	2.582941479586368E-5	0.0	3.427511684581518	0.0
31	2.582941479586368E-5	2.582941479586368E-5	0.0	3.514530983028783	0.0
32	2.582941479586368E-5	2.582941479586368E-5	0.0	3.6261915431913017	0.0
33	2.582941479586368E-5	2.582941479586368E-5	0.0	3.7264871608436403	0.0
34	2.582941479586368E-5	2.582941479586368E-5	0.0	3.8234249545725167	0.0
35	2.582941479586368E-5	2.582941479586368E-5	0.0	3.927207543222297	0.0
36	2.582941479586368E-5	2.582941479586368E-5	0.0	4.019056942236388	0.0
37	2.582941479586368E-5	2.582941479586368E-5	0.0	4.121522230731579	0.0
38	2.582941479586368E-5	2.582941479586368E-5	0.0	4.2486029515272286	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	30635	0.0	42.70018	1
GTTTTTT	34885	0.0	37.554825	2
TAATGCG	170	0.0	37.52941	1
GGGCGAT	8285	0.0	36.378998	7
GCGCGAC	1830	0.0	35.945354	9
ATTGGGA	2820	0.0	35.496452	4
TATGGGA	2960	0.0	35.452705	4
ACGATAG	180	0.0	35.444447	1
GTTGATC	2255	0.0	35.414635	16
ACGGGTA	330	0.0	35.333332	5
TAGGGAT	3705	0.0	35.271255	5
TAAGGGA	3690	0.0	35.176155	4
AGGGCGA	4395	0.0	35.089874	6
GGCGATA	1900	0.0	35.08421	8
ATAGGGA	4110	0.0	34.793186	4
ATAGGGC	2265	0.0	34.772625	4
TAGGGAC	2970	0.0	34.74074	5
GCCAGTA	2205	0.0	34.72109	25
CGTAAGG	775	0.0	34.63226	2
TACGGGT	305	0.0	34.62295	4
>>END_MODULE