FastQCFastQC Report
Thu 26 May 2016
SRR1547207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547207_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1829708
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232341.2698201024425755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTG207.857706E-444.01
CGCGTAG207.857706E-444.01
TCGAACG207.857706E-444.01
AATCGCG254.443877E-544.01
CGTTTTT229300.043.3859561
TGTAGCG1600.039.8751
CGTTAGG3350.038.746272
GCGATCG1500.038.133339
GTTTTTT257600.038.0388222
AGGGCGA17450.037.5701986
TGCGGGA12850.037.1517524
CGCGCAA1600.037.12518
CGTAAGG3100.036.9032252
TCGTTAG900.036.6666681
GTCGAAT301.3013966E-436.66666435
TATAGCG601.9826984E-1036.6666641
TAGGGCG7050.036.510645
TAGGGAC20550.036.5060845
TAGGGAT36050.036.493765
ACGGGAC6600.036.3333325
Produced by FastQC (version 0.11.5)