##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547207_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1829708
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.649561022851735	31.0	30.0	31.0	28.0	34.0
2	30.993510439917188	31.0	31.0	33.0	28.0	34.0
3	31.05834045651	31.0	31.0	34.0	28.0	34.0
4	34.90824765481705	35.0	35.0	37.0	32.0	37.0
5	34.73967758789927	35.0	35.0	37.0	32.0	37.0
6	34.78710810686732	35.0	35.0	37.0	32.0	37.0
7	35.50720224210639	36.0	35.0	37.0	35.0	37.0
8	34.485644157428396	37.0	35.0	37.0	33.0	37.0
9	36.59565132797146	39.0	37.0	39.0	32.0	39.0
10	36.01587247801289	38.0	35.0	39.0	30.0	39.0
11	35.7750646551253	37.0	35.0	39.0	30.0	39.0
12	35.70578966698511	37.0	35.0	39.0	30.0	39.0
13	35.70492176893799	37.0	35.0	39.0	30.0	39.0
14	36.674429471806434	38.0	35.0	40.0	31.0	41.0
15	36.747209390788036	38.0	35.0	40.0	31.0	41.0
16	36.73771443312266	38.0	35.0	40.0	31.0	41.0
17	36.633595633838844	38.0	35.0	40.0	30.0	41.0
18	36.44120318651938	38.0	35.0	40.0	30.0	41.0
19	36.31268213288678	38.0	35.0	40.0	30.0	41.0
20	36.07764189695842	38.0	34.0	40.0	30.0	41.0
21	35.94333631377247	38.0	34.0	40.0	30.0	41.0
22	35.900114663104716	37.0	34.0	40.0	30.0	41.0
23	35.746884202288015	37.0	34.0	40.0	29.0	41.0
24	35.628267461256115	37.0	34.0	40.0	29.0	41.0
25	35.4467007850433	37.0	34.0	40.0	27.0	41.0
26	35.28424098271418	37.0	34.0	40.0	27.0	41.0
27	35.10499598843094	37.0	33.0	40.0	27.0	41.0
28	35.09708871579509	37.0	33.0	40.0	27.0	41.0
29	34.94892245101404	37.0	33.0	40.0	26.0	41.0
30	34.85773249064878	37.0	33.0	40.0	25.0	41.0
31	34.69086979998994	37.0	33.0	40.0	25.0	41.0
32	34.23182660839872	37.0	33.0	40.0	23.0	41.0
33	33.79584228740324	37.0	33.0	40.0	21.0	41.0
34	33.43609909340726	37.0	32.0	40.0	18.0	41.0
35	33.091493833988814	37.0	31.0	40.0	16.0	41.0
36	32.96617820985644	37.0	31.0	40.0	15.0	41.0
37	32.934070354395345	37.0	31.0	40.0	15.0	41.0
38	32.913063723829154	37.0	31.0	40.0	15.0	41.0
39	32.80085182990947	37.0	31.0	40.0	14.0	41.0
40	32.755838090012176	37.0	31.0	40.0	13.0	41.0
41	32.75707162017109	37.0	31.0	40.0	13.0	41.0
42	32.60734991594287	37.0	31.0	40.0	12.0	41.0
43	32.64923310167524	37.0	31.0	40.0	12.0	41.0
44	32.36206323631968	36.0	30.0	40.0	10.0	41.0
45	32.298476040985776	36.0	30.0	40.0	10.0	41.0
46	32.23633005922257	36.0	30.0	40.0	10.0	41.0
47	32.18892194820157	36.0	30.0	40.0	10.0	41.0
48	32.16401688138217	36.0	30.0	40.0	10.0	41.0
49	32.15055353094592	36.0	30.0	40.0	10.0	41.0
50	31.64053171325698	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	26.0
15	90.0
16	303.0
17	859.0
18	2079.0
19	4065.0
20	6518.0
21	9912.0
22	14335.0
23	19855.0
24	28078.0
25	39447.0
26	53439.0
27	60025.0
28	59339.0
29	60199.0
30	65677.0
31	75668.0
32	89616.0
33	107864.0
34	136727.0
35	157072.0
36	164542.0
37	193933.0
38	238509.0
39	241525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.665334577976378	22.237045473922613	25.666335830635273	22.43128411746574
2	37.80739877619817	23.60403955166617	26.413613538335078	12.174948133800585
3	25.50505326532977	24.37197629348508	37.76077931560664	12.362191125578507
4	22.922837961029845	26.79252645777359	37.09154684791234	13.193088733284217
5	20.293183393197168	30.85951419570773	36.09985855666587	12.74744385442923
6	18.791960247208845	39.076726996876005	32.88562983820369	9.245682917711461
7	82.47310499817458	2.5308956401786515	14.045847752756178	0.950151608890599
8	80.89564017865146	4.843177162694812	13.27758308976077	0.98359956889296
9	77.35726137722521	5.425346558030024	15.037590697532066	2.1798013672126917
10	29.5024124067884	39.36005089336659	21.034230598543594	10.10330610130141
11	22.423304702171055	25.960918354185473	36.67076932494147	14.945007618702
12	22.088059952735627	23.81713366285768	37.534513703825965	16.560292680580726
13	20.43571979791311	25.621246668867382	38.002238608564866	15.940794924654645
14	18.326694751293648	28.698021760849272	36.86500796848458	16.11027551937249
15	18.049492050097612	26.375738642450052	40.244672920487865	15.33009638696448
16	20.396587870851523	25.45925360767948	39.181115238059846	14.963043283409155
17	20.454903186737994	24.276004695831247	36.61059578905487	18.658496328375893
18	20.53409615086123	24.514676658789273	37.86292676208444	17.088300428265057
19	22.22496704392176	25.562275510627924	35.39034643779226	16.822411007658054
20	23.532006199896376	25.790672610055815	34.674275895388774	16.00304529465904
21	22.628364744538473	25.52691467709602	36.10581579137217	15.738904786993334
22	21.667719658000074	24.06569791463993	35.96371661489155	18.30286581246844
23	19.045552623697333	27.36682574487295	36.132541367256415	17.4550802641733
24	18.785073902502475	26.29670963891506	37.87577034149711	17.04244611708535
25	18.884761940156572	27.467224278409454	36.26480290844222	17.383210872991757
26	18.76354041191272	29.114809576172814	35.02066996482499	17.100980047089482
27	18.702547073084887	27.634737346068334	36.23895178902863	17.423763791818146
28	17.96286620597385	27.30889300369239	38.087279500335576	16.640961289998184
29	18.839891392506345	25.687432092989702	37.45373578734967	18.018940727154277
30	19.376753011956005	26.29714686715039	36.683175676118815	17.642924444774792
31	20.702319714402517	26.71972795659198	34.873433356579305	17.7045189724262
32	21.224260920321715	26.849147514248173	34.7705754142191	17.156016151211013
33	21.045051997367885	27.40284242075785	33.84807849121281	17.704027090661462
34	19.78146239727869	28.436832543772013	33.5877637306062	18.1939413283431
35	20.00658028494164	28.794758507914924	32.851908610554254	18.346752596589184
36	21.32542460327003	29.423765977959327	31.33336029574118	17.917449123029467
37	20.881583290885757	29.895207322698486	31.28389885162004	17.939310534795716
38	20.61902773557311	30.201212433896558	30.646748005692707	18.533011824837626
39	20.825563423234748	29.665006656799882	30.30401572272734	19.20541419723803
40	21.39833241151047	28.850122533212954	30.80475135923328	18.946793696043304
41	19.546342913732683	29.211273055591384	30.773161619231047	20.46922241144489
42	20.73226984852228	29.604559853266206	29.811806036810246	19.851364261401272
43	20.866772184414124	29.188099959119164	30.188532815072133	19.756595041394583
44	20.556230830274558	29.997955957999856	30.708397186873533	18.737416024852052
45	20.444901590854933	30.060534249180744	29.782238477396394	19.71232568256793
46	20.430800980265705	30.41813229214716	29.738133079157986	19.41293364842915
47	20.441677032619413	29.162467453823233	30.47759533215136	19.918260181405994
48	20.900110837357655	28.6746847037888	31.02921340454324	19.395991054310304
49	20.5322379308611	28.240517066111092	31.25045089161768	19.976794111410126
50	20.065934017887006	29.46142225972669	30.70156549569658	19.771078226689724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	878.0
1	1285.0
2	1692.0
3	22396.5
4	43101.0
5	33002.0
6	22903.0
7	23134.0
8	23365.0
9	23938.0
10	24511.0
11	24019.5
12	23528.0
13	23080.5
14	22633.0
15	21554.5
16	20476.0
17	19757.5
18	19039.0
19	18045.0
20	17051.0
21	17045.0
22	17039.0
23	17909.5
24	18780.0
25	20826.5
26	22873.0
27	27230.5
28	31588.0
29	36709.5
30	41831.0
31	46917.0
32	52003.0
33	57868.5
34	63734.0
35	71587.0
36	79440.0
37	86017.5
38	92595.0
39	99642.5
40	106690.0
41	115019.5
42	123349.0
43	127220.5
44	131092.0
45	131092.0
46	131092.0
47	131174.5
48	131257.0
49	127567.5
50	123878.0
51	116122.0
52	108366.0
53	97898.0
54	87430.0
55	78600.0
56	69770.0
57	62217.5
58	54665.0
59	49752.5
60	44840.0
61	38958.0
62	33076.0
63	26555.0
64	20034.0
65	15504.5
66	10975.0
67	8387.5
68	5800.0
69	4545.5
70	3291.0
71	2580.0
72	1869.0
73	1504.0
74	1139.0
75	1063.0
76	987.0
77	776.0
78	565.0
79	428.0
80	291.0
81	196.5
82	102.0
83	67.0
84	32.0
85	36.0
86	40.0
87	27.0
88	14.0
89	8.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1829708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.984859575955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.47306538545278	24.8946846350603
2	6.450428105429619	4.2553293052477885
3	3.1269324704209858	3.0942428532099084
4	2.074432659556924	2.7369947990104
5	1.5689803438604895	2.5876298159835915
6	1.2486090262201017	2.471111603708398
7	1.0359719073414093	2.391997152180346
8	0.8395269188025405	2.215334202154878
9	0.7552106946287528	2.241946684132996
>10	7.211828670429601	46.70225909333642
>50	0.1963537730558668	4.039085335221245
>100	0.017987636122939802	0.9677078410920995
>500	5.043065085030606E-4	0.11339170101302277
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.6810216950102018E-4	1.28828497864869
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23234	1.2698201024425755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015248334706958706	0.0
2	0.0	0.0	0.0	0.05793274118055996	0.0
3	0.0	0.0	0.0	0.10766745294877653	0.0
4	0.0	0.0	0.0	0.1429189794218531	0.0
5	0.0	0.0	0.0	0.21555352001521555	0.0
6	0.0	0.0	0.0	0.40082898473417616	0.0
7	0.0	0.0	0.0	0.5028671241531436	0.0
8	0.0	0.0	0.0	0.7530709818178638	0.0
9	0.0	0.0	0.0	0.9243551430064251	0.0
10	0.0	0.0	0.0	1.1357003412566378	0.0
11	0.0	0.0	0.0	1.2628244506773758	0.0
12	0.0	0.0	0.0	1.3886915289215547	0.0
13	0.0	0.0	0.0	1.4891993695168846	0.0
14	0.0	0.0	0.0	1.5519416212860193	0.0
15	0.0	0.0	0.0	1.5998727665835204	0.0
16	0.0	0.0	0.0	1.6656209624705145	0.0
17	0.0	0.0	0.0	1.7368345112990706	0.0
18	0.0	0.0	0.0	1.8494207818952533	0.0
19	0.0	0.0	0.0	1.9074628301346443	0.0
20	0.0	0.0	0.0	1.9751785530806008	0.0
21	0.0	0.0	0.0	2.0419651660264915	0.0
22	0.0	0.0	0.0	2.110609998972514	0.0
23	0.0	0.0	0.0	2.190349498389907	0.0
24	0.0	0.0	0.0	2.2620002754537882	0.0
25	0.0	0.0	0.0	2.326163518987729	0.0
26	0.0	0.0	0.0	2.395081619580829	0.0
27	0.0	0.0	0.0	2.4748211189982228	0.0
28	0.0	0.0	0.0	2.550898831944769	0.0
29	0.0	0.0	0.0	2.632332590774047	0.0
30	0.0	0.0	0.0	2.740218657840486	0.0
31	0.0	0.0	0.0	2.829959753140938	0.0
32	0.0	0.0	0.0	2.9298664049126963	0.0
33	0.0	0.0	0.0	3.025783349037114	0.0
34	0.0	0.0	0.0	3.119076923749582	0.0
35	0.0	0.0	0.0	3.2182184261095212	0.0
36	0.0	0.0	0.0	3.3202565655284886	0.0
37	0.0	0.0	0.0	3.4213109414179748	0.0
38	0.0	0.0	0.0	3.5367938490731854	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTTG	20	7.857706E-4	44.0	1
CGCGTAG	20	7.857706E-4	44.0	1
TCGAACG	20	7.857706E-4	44.0	1
AATCGCG	25	4.443877E-5	44.0	1
CGTTTTT	22930	0.0	43.385956	1
TGTAGCG	160	0.0	39.875	1
CGTTAGG	335	0.0	38.74627	2
GCGATCG	150	0.0	38.13333	9
GTTTTTT	25760	0.0	38.038822	2
AGGGCGA	1745	0.0	37.570198	6
TGCGGGA	1285	0.0	37.151752	4
CGCGCAA	160	0.0	37.125	18
CGTAAGG	310	0.0	36.903225	2
TCGTTAG	90	0.0	36.666668	1
GTCGAAT	30	1.3013966E-4	36.666664	35
TATAGCG	60	1.9826984E-10	36.666664	1
TAGGGCG	705	0.0	36.51064	5
TAGGGAC	2055	0.0	36.506084	5
TAGGGAT	3605	0.0	36.49376	5
ACGGGAC	660	0.0	36.333332	5
>>END_MODULE