FastQCFastQC Report
Thu 26 May 2016
SRR1547206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547206_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1358257
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65660.48341366913625333No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15560.11455858501005334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.856987E-444.0000041
CTCCGAT207.856987E-444.00000440
ATCGACG302.528057E-644.040
CGTTTTT42150.040.398581
GCGTATG557.8216544E-1140.01
CATACGA2050.039.70731718
TATAGCG1000.039.61
CGAATAT850.038.8235314
GTATGCG800.038.5000041
CGACGGT2000.038.528
CGGTCTA1950.038.35897431
CTATGCG700.037.7142871
CGTTAGG2050.037.5609742
CGACAAT2000.037.39999820
ACGGTCT2000.037.39999830
GTAGGGC6600.037.3333324
TAACGGG3250.037.230773
CTAACGG651.0913936E-1137.230772
TCGACAA1950.037.2307719
TAGGGCG6650.036.7218065
Produced by FastQC (version 0.11.5)