##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547206_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1358257
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.587547128415313	31.0	30.0	31.0	28.0	33.0
2	30.908507005669765	31.0	31.0	33.0	28.0	34.0
3	31.08315583869621	31.0	31.0	34.0	28.0	34.0
4	34.90961725211061	35.0	35.0	37.0	32.0	37.0
5	34.7013392899871	35.0	35.0	37.0	32.0	37.0
6	34.738687891908526	35.0	35.0	37.0	32.0	37.0
7	35.35943271413289	35.0	35.0	37.0	33.0	37.0
8	34.31892049884521	35.0	35.0	37.0	32.0	37.0
9	36.414594587033235	39.0	37.0	39.0	32.0	39.0
10	35.8402084436156	37.0	35.0	39.0	30.0	39.0
11	35.60142962635201	37.0	35.0	39.0	30.0	39.0
12	35.08953165711644	37.0	34.0	39.0	30.0	39.0
13	34.85478079627052	37.0	33.0	39.0	27.0	39.0
14	35.728639719876284	38.0	34.0	40.0	27.0	41.0
15	35.96102357653964	38.0	34.0	40.0	29.0	41.0
16	36.0640836012625	38.0	34.0	40.0	30.0	41.0
17	35.983569383408295	38.0	34.0	40.0	30.0	41.0
18	35.909241034649554	38.0	34.0	40.0	29.0	41.0
19	35.89815403123268	37.0	34.0	40.0	29.0	41.0
20	35.75710855898405	37.0	34.0	40.0	29.0	41.0
21	35.56672853517412	37.0	34.0	40.0	29.0	41.0
22	35.526199386419506	37.0	34.0	40.0	29.0	41.0
23	35.43328545334204	37.0	34.0	40.0	28.0	41.0
24	35.331786252528055	37.0	34.0	40.0	27.0	41.0
25	35.116315248145234	37.0	33.0	40.0	27.0	41.0
26	34.91720639024868	37.0	33.0	40.0	27.0	41.0
27	34.68615733252249	36.0	33.0	40.0	26.0	41.0
28	34.713702929563404	36.0	33.0	40.0	26.0	41.0
29	34.53714208724858	36.0	33.0	40.0	25.0	41.0
30	34.56374014637878	36.0	33.0	40.0	25.0	41.0
31	34.51178458863087	36.0	33.0	40.0	25.0	41.0
32	34.304155988152466	36.0	33.0	40.0	24.0	41.0
33	34.186299058278365	36.0	33.0	40.0	24.0	41.0
34	34.06649993337049	36.0	33.0	40.0	23.0	41.0
35	33.88812794633122	36.0	32.0	40.0	23.0	41.0
36	33.81787246448942	36.0	32.0	40.0	23.0	41.0
37	33.816519259609926	36.0	32.0	40.0	23.0	41.0
38	33.83456223674901	36.0	32.0	40.0	23.0	41.0
39	33.725324441545304	36.0	32.0	40.0	23.0	41.0
40	33.634103855161435	36.0	32.0	40.0	23.0	41.0
41	33.63713936316912	36.0	32.0	40.0	23.0	41.0
42	33.479525597880226	35.0	32.0	40.0	22.0	41.0
43	33.522309842688095	35.0	32.0	39.0	23.0	41.0
44	33.21623448287033	35.0	31.0	39.0	22.0	41.0
45	33.129523352355264	35.0	31.0	39.0	22.0	41.0
46	33.07534582925028	35.0	31.0	39.0	22.0	41.0
47	32.98450293280285	35.0	31.0	39.0	22.0	41.0
48	32.97905624635102	35.0	31.0	39.0	22.0	40.0
49	32.98266307480838	35.0	31.0	39.0	22.0	40.0
50	32.42660851370543	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	4.0
13	10.0
14	20.0
15	78.0
16	236.0
17	533.0
18	1208.0
19	2288.0
20	3868.0
21	5849.0
22	8693.0
23	12027.0
24	16599.0
25	21952.0
26	28152.0
27	33924.0
28	39358.0
29	46009.0
30	54686.0
31	64872.0
32	78029.0
33	94708.0
34	129327.0
35	140694.0
36	123721.0
37	141477.0
38	162200.0
39	147732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.97806379794104	23.852628773494263	27.083607888639634	14.085699539925065
2	31.648428831951534	25.888620489347748	28.567568582381686	13.89538209631903
3	29.975475922450613	26.31180991520751	30.225428619178846	13.487285543163038
4	26.384918318109165	29.06187856937236	29.425727237187072	15.1274758753314
5	22.794655208844866	32.46800863165071	29.488528312388596	15.248807847115826
6	21.65157256690008	41.774862930947535	26.24260357207804	10.330960930074353
7	88.41552077405086	3.5953431493450796	6.380162222613246	1.6089738539908132
8	86.26173102733871	5.922811367804473	5.950125786209826	1.8653318186469865
9	83.30860801748122	5.136877630669306	8.306896264845314	3.2476180870041533
10	43.08161121201658	29.494565461470106	14.688899081690726	12.734924244822594
11	37.85130501812249	24.53828693686099	22.193369885080656	15.417038159935858
12	34.09987947788968	22.08860326138573	26.296128052349445	17.515389208375147
13	24.62332239038709	29.355563785056876	27.800997896568912	18.22011592798712
14	20.625698965659662	32.211650666994544	29.172976837225946	17.989673530119852
15	20.078232617244012	23.267393431434552	38.337221895414494	18.317152055906945
16	22.75438300704506	21.122364913267518	36.468356135841745	19.65489594384568
17	22.9529463128112	21.21314302079798	28.57979012808327	27.254120538307554
18	24.595566229366018	23.06956636336128	31.270370776664503	21.064496630608197
19	29.50884847271172	24.31388168807523	25.888694113117033	20.28857572609602
20	30.53052551910279	24.331551392704032	25.33923992293064	19.79868316526254
21	26.386611664802757	26.151015603085426	27.618263701199403	19.844109030912414
22	25.535447268079604	23.65944000288605	26.861779471778906	23.94333325725544
23	24.042578098253863	26.463254008630177	26.423055430599657	23.0711124625163
24	23.254288400501526	24.322274797773915	32.27297926681033	20.15045753491423
25	23.458741607810598	24.5156108159207	30.260620780897874	21.765026795370833
26	21.579126777922	28.51824065695962	27.014401545510164	22.88823101960822
27	21.223376724728826	28.245317344213944	28.242666888519626	22.288639042537607
28	20.929470637736454	26.819593051977648	31.67795196343549	20.57298434685041
29	22.408130420089865	25.356983251328725	30.8954785434568	21.33940778512461
30	23.48406818444521	26.633398539451665	29.228341911729515	20.654191364373606
31	27.725018166665073	25.50489340382564	26.123333065833638	20.646755363675652
32	28.924938358499162	24.92665231984816	26.32425233221695	19.824156989435725
33	28.038729047595556	25.192875869588743	25.336736714774894	21.431658368040804
34	23.125078685403423	26.52355187567596	28.227279520738712	22.124089918181905
35	23.308107375850078	26.1434323548489	28.59723896140421	21.951221307896812
36	27.412927008658894	27.015285030741605	25.162910995489074	20.40887696511043
37	23.59538732360665	30.005072677703854	26.05243337601058	20.34710662267892
38	24.247104929332224	29.848180425353966	24.278247783740486	21.626466861573327
39	23.85954940780721	28.85079922282749	24.6127941913791	22.6768571779862
40	25.1252156256143	24.822842805153957	26.57103920686586	23.480902362365885
41	21.462359479833346	25.02729601246303	27.13691149760318	26.373433010100445
42	23.030104023023625	26.081441141109522	25.1693898871863	25.719064948680554
43	22.535794036032943	25.61201598813774	27.00674467350435	24.845445302324965
44	22.709398884010906	26.642086144227488	27.920710145429034	22.72780482633257
45	21.8706769042972	28.958584421063172	26.012971035672926	23.157767638966707
46	23.48605602621595	28.144158285214065	26.87186592817118	21.497919760398805
47	22.797158417000613	27.239690279527363	26.77865823625426	23.184493067217765
48	23.238385666335606	25.674669815800694	28.77386238392293	22.313082133940778
49	23.01780885355275	25.606567829210526	28.767236244687123	22.6083870725496
50	22.29865187516059	28.44181918444006	26.85206113423306	22.407467806166284
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	816.0
1	1372.5
2	1929.0
3	6821.0
4	11713.0
5	8498.5
6	5284.0
7	5281.0
8	5278.0
9	5577.0
10	5876.0
11	5807.5
12	5739.0
13	5692.5
14	5646.0
15	5542.0
16	5438.0
17	5674.5
18	5911.0
19	5603.5
20	5296.0
21	6109.5
22	6923.0
23	7656.0
24	8389.0
25	9044.5
26	9700.0
27	11170.5
28	12641.0
29	15206.0
30	17771.0
31	20997.0
32	24223.0
33	27083.5
34	29944.0
35	34600.0
36	39256.0
37	43174.0
38	47092.0
39	51984.5
40	56877.0
41	67759.0
42	78641.0
43	86966.5
44	95292.0
45	95615.5
46	95939.0
47	94436.0
48	92933.0
49	93501.0
50	94069.0
51	92182.0
52	90295.0
53	83651.0
54	77007.0
55	74087.5
56	71168.0
57	71659.0
58	72150.0
59	69673.0
60	67196.0
61	62928.5
62	58661.0
63	53365.0
64	48069.0
65	40527.0
66	32985.0
67	27966.5
68	22948.0
69	20586.0
70	18224.0
71	15395.5
72	12567.0
73	10284.5
74	8002.0
75	6237.5
76	4473.0
77	3902.5
78	3332.0
79	2291.0
80	1250.0
81	987.5
82	725.0
83	539.5
84	354.0
85	250.5
86	147.0
87	101.0
88	55.0
89	38.0
90	21.0
91	15.0
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1358257.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.93587051329914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.092555255669	19.473058459080203
2	6.573281117737967	3.278209735988564
3	2.51867878544665	1.8841634417547362
4	1.319226066604815	1.3158420149850645
5	0.9154687798136816	1.1414005476200961
6	0.6836805235759723	1.0228901405013002
7	0.5563716252141304	0.9711527562529348
8	0.4821118647281522	0.9617503225429114
9	0.4174614567450239	0.936878834671843
>10	6.984221060146778	43.26500884596462
>50	1.379232471406149	22.50763270924896
>100	0.07654240655234093	2.5645137446248247
>500	5.842931796754683E-4	0.0858164699234268
>1k	2.921465898377342E-4	0.11335350356608836
>5k	2.921465898377342E-4	0.4783284732743806
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6566	0.48341366913625333	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1556	0.11455858501005334	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009865585084413332	0.0
2	0.0	0.0	0.0	0.043143528801986665	0.0
3	0.0	0.0	0.0	0.07156230374664	0.0
4	0.0	0.0	0.0	0.1148530800872	0.0
5	0.0	0.0	0.0	0.19480849353252	0.0
6	0.0	0.0	0.0	0.3581060138103466	0.0
7	0.0	0.0	0.0	0.43887128871782	0.0
8	0.0	0.0	0.0	0.6572393884220733	0.0
9	0.0	0.0	0.0	0.7662025669663399	0.0
10	0.0	0.0	0.0	0.9353163650178132	0.0
11	0.0	0.0	0.0	1.0536297622614865	0.0
12	0.0	0.0	0.0	1.1621511981900332	0.0
13	7.362376928666666E-5	0.0	0.0	1.2167800350007398	0.0
14	7.362376928666666E-5	0.0	0.0	1.2456405525611132	0.0
15	7.362376928666666E-5	0.0	0.0	1.2745746938907732	0.0
16	7.362376928666666E-5	0.0	0.0	1.3336945806279665	0.0
17	7.362376928666666E-5	0.0	0.0	1.3936979525966	0.0
18	7.362376928666666E-5	0.0	0.0	1.4891879813614066	0.0
19	7.362376928666666E-5	0.0	0.0	1.5293129356226398	0.0
20	7.362376928666666E-5	0.0	0.0	1.5770947618896867	0.0
21	7.362376928666666E-5	0.0	0.0	1.6325334601625465	0.0
22	7.362376928666666E-5	0.0	0.0	1.6906226141297265	0.0
23	7.362376928666666E-5	0.0	0.0	1.7602706998749131	0.0
24	1.4724753857333332E-4	0.0	0.0	1.8141632989927532	0.0
25	1.4724753857333332E-4	0.0	0.0	1.8618715014905132	0.0
26	1.4724753857333332E-4	0.0	0.0	1.9114202982204398	0.0
27	1.4724753857333332E-4	0.0	0.0	1.9645030358761266	0.0
28	1.4724753857333332E-4	0.0	0.0	2.016481416992513	0.0
29	1.4724753857333332E-4	0.0	0.0	2.0780308881161664	0.0
30	1.4724753857333332E-4	0.0	0.0	2.1555567171750267	0.0
31	1.4724753857333332E-4	0.0	0.0	2.2247630603044932	0.0
32	1.4724753857333332E-4	0.0	0.0	2.2951473837425467	0.0
33	1.4724753857333332E-4	0.0	0.0	2.366783311258473	0.0
34	1.4724753857333332E-4	0.0	0.0	2.4424685460851667	0.0
35	1.4724753857333332E-4	0.0	0.0	2.5257370291483867	0.0
36	1.4724753857333332E-4	0.0	0.0	2.6026002442836664	0.0
37	1.4724753857333332E-4	0.0	0.0	2.6783591028796465	0.0
38	1.4724753857333332E-4	0.0	0.0	2.7693580817179666	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.856987E-4	44.000004	1
CTCCGAT	20	7.856987E-4	44.000004	40
ATCGACG	30	2.528057E-6	44.0	40
CGTTTTT	4215	0.0	40.39858	1
GCGTATG	55	7.8216544E-11	40.0	1
CATACGA	205	0.0	39.707317	18
TATAGCG	100	0.0	39.6	1
CGAATAT	85	0.0	38.82353	14
GTATGCG	80	0.0	38.500004	1
CGACGGT	200	0.0	38.5	28
CGGTCTA	195	0.0	38.358974	31
CTATGCG	70	0.0	37.714287	1
CGTTAGG	205	0.0	37.560974	2
CGACAAT	200	0.0	37.399998	20
ACGGTCT	200	0.0	37.399998	30
GTAGGGC	660	0.0	37.333332	4
TAACGGG	325	0.0	37.23077	3
CTAACGG	65	1.0913936E-11	37.23077	2
TCGACAA	195	0.0	37.23077	19
TAGGGCG	665	0.0	36.721806	5
>>END_MODULE