FastQCFastQC Report
Thu 26 May 2016
SRR1547205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547205_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4473079
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT551671.233311551170905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT422950.042.4707371
GTTTTTT480400.037.171942
AGGGCGA48100.037.1392946
GGGCGAT94050.037.0760237
TAGGGAT58250.037.0506445
CGTAAGG6700.036.447762
TAGGGAC42500.036.3905875
ATAGGGA56200.035.857654
TACGGGA15500.035.6258054
TAAGGGA53450.035.479894
ACCCGCT22350.035.4362434
TAGCCGT3050.035.3442644
AGGGATT67900.035.2518426
GCGCGAC22550.035.2195139
CGTATCG250.002353469635.236
AAGGGAT66750.035.25
CAATCGA250.002353469635.240
GGCGATA19350.035.1317838
TTAGGGA56950.035.1150134
GTAGGGA54650.035.1033864
Produced by FastQC (version 0.11.5)