##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547205_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4473079
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.566893855440515	31.0	30.0	31.0	27.0	34.0
2	30.90907627609528	31.0	31.0	33.0	28.0	34.0
3	31.022704942166236	31.0	31.0	34.0	28.0	34.0
4	34.87118559721391	35.0	35.0	37.0	32.0	37.0
5	34.68519558898915	35.0	35.0	37.0	32.0	37.0
6	34.718088368213486	35.0	35.0	37.0	31.0	37.0
7	35.38034338315956	35.0	35.0	37.0	33.0	37.0
8	34.34739784385655	35.0	35.0	37.0	32.0	37.0
9	36.444166758512424	39.0	37.0	39.0	32.0	39.0
10	35.87960261824126	38.0	35.0	39.0	30.0	39.0
11	35.638712618310564	37.0	35.0	39.0	30.0	39.0
12	35.381396796256	37.0	35.0	39.0	30.0	39.0
13	35.3073160567922	37.0	34.0	39.0	30.0	39.0
14	36.194618740245815	38.0	35.0	40.0	30.0	41.0
15	36.320455999100396	38.0	34.0	40.0	30.0	41.0
16	36.358556600498225	38.0	34.0	40.0	30.0	41.0
17	36.24972574819269	38.0	34.0	40.0	30.0	41.0
18	36.10828134267246	38.0	34.0	40.0	30.0	41.0
19	35.9984095966112	37.0	34.0	40.0	29.0	41.0
20	35.781400909753664	37.0	34.0	40.0	29.0	41.0
21	35.62246966798485	37.0	34.0	40.0	29.0	41.0
22	35.58034566346805	37.0	34.0	40.0	29.0	41.0
23	35.44231434320744	37.0	34.0	40.0	28.0	41.0
24	35.311371652501556	37.0	34.0	40.0	27.0	41.0
25	35.10962337128407	37.0	33.0	40.0	27.0	41.0
26	34.912057891219895	36.0	33.0	40.0	27.0	41.0
27	34.72523758243483	36.0	33.0	40.0	26.0	41.0
28	34.69033656682567	36.0	33.0	40.0	25.0	41.0
29	34.4725373730265	36.0	33.0	40.0	25.0	41.0
30	34.398173830598566	36.0	33.0	40.0	24.0	41.0
31	34.283657409135856	36.0	33.0	40.0	24.0	41.0
32	33.914292369976025	36.0	32.0	40.0	23.0	41.0
33	33.6011856709886	36.0	32.0	40.0	21.0	41.0
34	33.30368902494233	36.0	31.0	40.0	19.0	41.0
35	33.004496008230575	36.0	31.0	40.0	18.0	41.0
36	32.88362356220402	36.0	31.0	40.0	18.0	41.0
37	32.87700664352228	36.0	31.0	40.0	17.0	41.0
38	32.84254894670986	36.0	31.0	40.0	17.0	41.0
39	32.70823318792268	36.0	31.0	40.0	16.0	41.0
40	32.61838881003443	36.0	31.0	40.0	15.0	41.0
41	32.60176267845929	36.0	31.0	40.0	15.0	41.0
42	32.434591474910235	35.0	30.0	40.0	15.0	41.0
43	32.46166700834034	35.0	30.0	40.0	15.0	41.0
44	32.06709628870851	35.0	30.0	39.0	14.0	41.0
45	32.026852420893974	35.0	30.0	39.0	14.0	41.0
46	31.985835707350574	35.0	30.0	39.0	14.0	41.0
47	31.926611401229444	35.0	30.0	39.0	14.0	41.0
48	31.918166435245162	35.0	30.0	39.0	14.0	41.0
49	31.91576495742642	35.0	30.0	39.0	15.0	40.0
50	31.40075393258201	35.0	29.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	3.0
12	11.0
13	30.0
14	94.0
15	324.0
16	1018.0
17	2561.0
18	5777.0
19	10760.0
20	17478.0
21	26055.0
22	37276.0
23	51225.0
24	69612.0
25	95636.0
26	125059.0
27	144393.0
28	150623.0
29	159916.0
30	177912.0
31	204085.0
32	239628.0
33	285917.0
34	368989.0
35	408279.0
36	403296.0
37	462113.0
38	530414.0
39	494589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.480754084602573	22.903105444817765	25.75771632917728	19.858424141402374
2	35.84164285942636	24.109835752956744	26.815444126964895	13.233077260652001
3	27.438840226161894	24.527288697561566	34.8823036659983	13.151567410278245
4	24.35304630211092	27.043519687445716	34.235210243324566	14.368223767118801
5	21.56127803689584	30.696909220695634	33.68525349093991	14.05655925146862
6	19.88681621764337	39.486268854182995	30.874169671494734	9.752745256678901
7	83.34842733606985	3.254536751977776	12.033210233935058	1.363825678017312
8	81.36712988972474	5.458410191279877	11.581955069427568	1.5925048495678256
9	78.22419411774307	5.250030236443398	13.701233535110827	2.8245421107027173
10	36.77554096406524	31.829864842539113	19.486018467368897	11.908575726026747
11	29.037917729599677	23.926293275839754	31.06607327972522	15.969715714835353
12	26.553767550271303	21.43047328249736	34.05175719006975	17.964001977161594
13	23.513199744516026	24.265500341040255	34.36044836230256	17.860851552141156
14	20.398231285430015	26.58014758961333	34.52273031618713	18.49889080876953
15	19.510431181743044	22.718802864872274	38.958109168203826	18.812656785180856
16	22.929172500642174	21.48330042907805	36.824388748779086	18.763138321500694
17	22.03439733570545	21.478382116658345	34.22400990458698	22.263210643049227
18	23.315192957692005	21.869566801748864	35.391214865644	19.42402537491513
19	25.436103408859985	24.25067833588452	31.19233977311825	19.12087848213725
20	26.75188611692304	23.82504310789056	30.916668361994056	18.506402413192344
21	24.865981575554557	24.586599074150044	32.670940978238924	17.87647837205647
22	23.980886543698425	21.879246934829453	32.51733313898547	21.62253338248665
23	21.72027366384542	25.01516293363028	32.443021909516915	20.821541493007388
24	21.43802959885126	23.805772265591553	35.74922329786708	19.0069748376901
25	22.36325358885904	24.137266522679347	33.10303708027513	20.396442808186485
26	20.589598350487435	25.975284585852386	32.186531916829544	21.248585146830628
27	20.822033324249357	24.302767735602256	33.3224161701593	21.552782769989083
28	20.290721447128476	25.092872269861545	34.35336152122509	20.26304476178489
29	21.67138116720049	23.845141120914697	34.03892486584744	20.44455284603737
30	21.99657104200485	24.234090209450805	33.6943747248819	20.07496402366245
31	24.515104696340035	24.587739228392792	31.260525468027726	19.636630607239443
32	24.47461804273969	24.31050289968051	31.068510079969524	20.146368977610276
33	24.523018708142647	24.71011578378115	29.863501181177437	20.903364326898767
34	21.790516107584953	26.085410072122578	30.878596152672465	21.24547766762
35	21.832813594394377	26.67887153345604	30.654790581610563	20.83352429053902
36	24.006171140728792	27.825486650246955	27.47201647902932	20.696325729994932
37	22.16649873610549	28.863652978183485	28.147278418288614	20.822569867422416
38	23.055886113346087	28.624578282654966	27.5939459151068	20.72558968889215
39	22.518515769562754	27.454332910283945	27.82396644458996	22.20318487556334
40	23.811607172598563	25.505183342391224	29.01929521030145	21.663914274708763
41	21.272438962066172	25.728273522555718	29.21895633857573	23.780331176802378
42	22.159389539062467	26.64936165893784	27.75160018412373	23.439648617875964
43	21.7220397851234	25.715910673609834	29.02392289516908	23.538126646097687
44	21.89775767429996	26.24523286979729	29.787669746051883	22.069339709850865
45	21.43134516515358	27.1943553869717	28.42652678389986	22.94777266397486
46	22.252636271346873	27.17783432843462	29.020904839820627	21.548624560397883
47	21.530493872341623	26.845758816242682	29.59131730067812	22.03243001073757
48	21.72586265523144	25.973272548953418	30.64229359687142	21.658571198943726
49	22.34970587373932	25.445671762112852	30.47113632466585	21.733486039481978
50	21.141567139771063	26.844037406895787	30.01384504946146	22.000550403871696
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2708.0
1	4424.0
2	6140.0
3	51655.5
4	97171.0
5	71048.5
6	44926.0
7	44612.0
8	44298.0
9	44964.0
10	45630.0
11	45151.0
12	44672.0
13	43767.5
14	42863.0
15	40815.5
16	38768.0
17	36876.5
18	34985.0
19	33853.5
20	32722.0
21	31967.5
22	31213.0
23	31946.0
24	32679.0
25	35660.5
26	38642.0
27	44266.0
28	49890.0
29	59709.0
30	69528.0
31	78890.5
32	88253.0
33	99112.5
34	109972.0
35	124074.5
36	138177.0
37	147009.0
38	155841.0
39	175433.5
40	195026.0
41	213306.5
42	231587.0
43	250238.5
44	268890.0
45	280069.5
46	291249.0
47	295734.5
48	300220.0
49	293558.0
50	286896.0
51	271697.0
52	256498.0
53	249655.0
54	242812.0
55	230286.0
56	217760.0
57	217337.5
58	216915.0
59	207660.5
60	198406.0
61	183229.5
62	168053.0
63	150460.0
64	132867.0
65	115351.0
66	97835.0
67	82476.5
68	67118.0
69	60013.0
70	52908.0
71	46290.0
72	39672.0
73	32747.5
74	25823.0
75	20780.0
76	15737.0
77	12395.0
78	9053.0
79	6947.0
80	4841.0
81	3428.0
82	2015.0
83	1551.5
84	1088.0
85	772.5
86	457.0
87	304.0
88	151.0
89	117.5
90	84.0
91	52.5
92	21.0
93	15.0
94	9.0
95	9.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4473079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.26271969837741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.33687055408386	20.547905619510278
2	5.46530935402977	2.761371565515484
3	2.045030397988272	1.549890891571167
4	1.1646957099794721	1.176935250204563
5	0.8376762459265554	1.0580990099415817
6	0.589754699231137	0.8939284594486254
7	0.5130113516223205	0.907204338468429
8	0.4136062799390377	0.8359055612470823
9	0.37958779448750557	0.8630478047756829
>10	5.5764516102353445	35.24959345790405
>50	1.4785314174768185	25.18299015828127
>100	0.19531340319053864	6.768246349612932
>500	0.0027138142251564644	0.43913894400775366
>1k	0.0013569071102283962	0.5050234965652247
>5k	0.0	0.0
>10k+	9.04604740152264E-5	1.2607190929460044
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55167	1.233311551170905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011110914875413557	0.0
2	0.0	0.0	0.0	0.045315542157873805	0.0
3	0.0	0.0	0.0	0.0879930803815448	0.0
4	0.0	0.0	0.0	0.1375339000272519	0.0
5	0.0	0.0	0.0	0.22443153809713623	0.0
6	0.0	0.0	0.0	0.45402730423495763	0.0
7	0.0	0.0	0.0	0.6003918106521258	0.0
8	0.0	0.0	0.0	0.9588250062205474	0.0
9	0.0	0.0	0.0	1.1770862978275143	0.0
10	0.0	0.0	0.0	1.4482418039118021	0.0
11	0.0	0.0	0.0	1.6015590156131827	0.0
12	0.0	0.0	0.0	1.7335262802199558	0.0
13	0.0	0.0	0.0	1.8148349269038173	0.0
14	0.0	0.0	0.0	1.8600386892339706	0.0
15	0.0	0.0	0.0	1.8943774523096955	0.0
16	0.0	0.0	0.0	1.952570030620966	0.0
17	0.0	0.0	0.0	2.0117462714161767	0.0
18	0.0	0.0	0.0	2.107921635186859	0.0
19	0.0	0.0	0.0	2.153952568242144	0.0
20	0.0	0.0	0.0	2.205460712855731	0.0
21	0.0	0.0	0.0	2.262468424993165	0.0
22	0.0	0.0	0.0	2.3262947066215465	0.0
23	0.0	0.0	0.0	2.399778765364976	0.0
24	0.0	0.0	0.0	2.4629790799581226	0.0
25	0.0	0.0	0.0	2.5190925534737927	0.0
26	0.0	0.0	0.0	2.5760779096456825	0.0
27	0.0	0.0	0.0	2.6395017838942705	0.0
28	0.0	0.0	0.0	2.703462201315917	0.0
29	0.0	0.0	0.0	2.7727209825715127	0.0
30	0.0	0.0	0.0	2.8711766548276927	0.0
31	2.2355965544091666E-5	0.0	0.0	2.950294416888233	0.0
32	2.2355965544091666E-5	0.0	0.0	3.0384887009596744	0.0
33	2.2355965544091666E-5	0.0	0.0	3.122323571750018	0.0
34	2.2355965544091666E-5	0.0	0.0	3.2126193165826047	0.0
35	2.2355965544091666E-5	0.0	0.0	3.314450739635942	0.0
36	2.2355965544091666E-5	0.0	0.0	3.4125710724089604	0.0
37	2.2355965544091666E-5	0.0	0.0	3.513709460530431	0.0
38	2.2355965544091666E-5	0.0	0.0	3.634878793779408	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	42295	0.0	42.470737	1
GTTTTTT	48040	0.0	37.17194	2
AGGGCGA	4810	0.0	37.139294	6
GGGCGAT	9405	0.0	37.076023	7
TAGGGAT	5825	0.0	37.050644	5
CGTAAGG	670	0.0	36.44776	2
TAGGGAC	4250	0.0	36.390587	5
ATAGGGA	5620	0.0	35.85765	4
TACGGGA	1550	0.0	35.625805	4
TAAGGGA	5345	0.0	35.47989	4
ACCCGCT	2235	0.0	35.43624	34
TAGCCGT	305	0.0	35.34426	44
AGGGATT	6790	0.0	35.251842	6
GCGCGAC	2255	0.0	35.219513	9
CGTATCG	25	0.0023534696	35.2	36
AAGGGAT	6675	0.0	35.2	5
CAATCGA	25	0.0023534696	35.2	40
GGCGATA	1935	0.0	35.131783	8
TTAGGGA	5695	0.0	35.115013	4
GTAGGGA	5465	0.0	35.103386	4
>>END_MODULE