FastQCFastQC Report
Thu 26 May 2016
SRR1547204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547204_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences737821
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122581.6613785728516808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATG207.8546465E-444.01
ACGAACG207.8546465E-444.01
CGTTTTT95350.042.89251
GTTTTTT105200.038.6254772
GCCGATA357.2866624E-637.7142879
AGTACGG357.2866624E-637.7142872
GGCACCG3050.037.5081988
GACCGAT5000.037.49
GTTACGG651.0913936E-1137.230772
AACGTAG301.3006433E-436.6666681
GCGCGAC1800.036.6666689
TGCGACG301.3006433E-436.6666681
TTACGGG1500.036.6666683
AATCGTT301.3006433E-436.66666822
ATAGGGA9100.036.0219764
TACGAAT553.421519E-936.00000412
CGAATAT553.421519E-936.00000414
CGACCCG1900.035.89473732
TAGGGAT10650.035.7370875
TGGGCGA4250.035.7176486
Produced by FastQC (version 0.11.5)