##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547204_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	737821
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.62252904159681	31.0	30.0	31.0	27.0	34.0
2	30.96760325336362	31.0	31.0	33.0	28.0	34.0
3	31.02493829804248	31.0	31.0	34.0	28.0	34.0
4	34.888472949401006	35.0	35.0	37.0	32.0	37.0
5	34.7268849761663	35.0	35.0	37.0	32.0	37.0
6	34.764852179593696	35.0	35.0	37.0	32.0	37.0
7	35.45133033621976	35.0	35.0	37.0	35.0	37.0
8	34.426389327492714	36.0	35.0	37.0	32.0	37.0
9	36.52910258721289	39.0	37.0	39.0	32.0	39.0
10	35.9573162054211	38.0	35.0	39.0	30.0	39.0
11	35.71909853473946	37.0	35.0	39.0	30.0	39.0
12	35.59500746115928	37.0	35.0	39.0	30.0	39.0
13	35.58233907682216	37.0	35.0	39.0	30.0	39.0
14	36.549272791097025	38.0	35.0	40.0	30.0	41.0
15	36.62632942136372	38.0	35.0	40.0	30.0	41.0
16	36.620421484343765	38.0	35.0	40.0	30.0	41.0
17	36.50544915365651	38.0	35.0	40.0	30.0	41.0
18	36.28720380688541	38.0	35.0	40.0	30.0	41.0
19	36.1370115515823	37.0	34.0	40.0	30.0	41.0
20	35.88566332484437	37.0	34.0	40.0	30.0	41.0
21	35.757748830678445	37.0	34.0	40.0	29.0	41.0
22	35.70632172301954	37.0	34.0	40.0	29.0	41.0
23	35.55868835395035	37.0	34.0	40.0	29.0	41.0
24	35.39916592235786	37.0	34.0	40.0	28.0	41.0
25	35.22298362339917	37.0	33.0	40.0	27.0	41.0
26	35.03242927485122	37.0	33.0	40.0	27.0	41.0
27	34.84335631542068	36.0	33.0	40.0	26.0	41.0
28	34.80672412414393	36.0	33.0	40.0	26.0	41.0
29	34.59947331398808	36.0	33.0	40.0	25.0	41.0
30	34.501242171204126	36.0	33.0	40.0	25.0	41.0
31	34.3376347379649	36.0	33.0	40.0	24.0	41.0
32	33.88967649335002	36.0	33.0	40.0	22.0	41.0
33	33.4578685074022	36.0	32.0	40.0	20.0	41.0
34	33.07593576219706	36.0	31.0	40.0	18.0	41.0
35	32.73194311357362	36.0	31.0	40.0	15.0	41.0
36	32.612575407856376	36.0	31.0	40.0	15.0	41.0
37	32.583808267859006	36.0	31.0	40.0	15.0	41.0
38	32.54322660916401	36.0	31.0	40.0	14.0	41.0
39	32.42156701964298	36.0	30.0	40.0	12.0	41.0
40	32.34763851936987	36.0	30.0	40.0	12.0	41.0
41	32.32146685984812	36.0	30.0	40.0	12.0	41.0
42	32.156542033907954	36.0	30.0	40.0	10.0	41.0
43	32.19559215582099	36.0	30.0	40.0	10.0	41.0
44	31.89794137060344	35.0	30.0	40.0	10.0	41.0
45	31.840530426756626	35.0	30.0	39.0	10.0	41.0
46	31.767954558083872	35.0	29.0	39.0	10.0	41.0
47	31.716134401162343	35.0	29.0	39.0	10.0	41.0
48	31.679518473993014	35.0	29.0	39.0	10.0	41.0
49	31.672065446768254	35.0	29.0	39.0	10.0	41.0
50	31.159549538438185	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	2.0
14	18.0
15	45.0
16	158.0
17	371.0
18	878.0
19	1798.0
20	3010.0
21	4375.0
22	6234.0
23	8774.0
24	11973.0
25	16859.0
26	22752.0
27	25980.0
28	25498.0
29	26116.0
30	28254.0
31	32040.0
32	37522.0
33	44707.0
34	57125.0
35	64530.0
36	65947.0
37	76330.0
38	89803.0
39	86720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.750169756621187	22.119186089851063	24.819027921406413	23.31161623212134
2	39.10270919369332	23.262417307178843	25.77766151952845	11.857211979599388
3	26.220316309782454	23.916776562336935	37.44539664769639	12.417510480184218
4	23.026452215374732	26.407895682015013	37.07755675156982	13.488095351040426
5	20.714373811534234	29.825526787662593	36.46724612067154	12.99285328013163
6	19.268494661984413	38.06682108533099	33.57196393163112	9.09272032105348
7	80.94483621366157	2.837409073474461	15.073168153251263	1.1445865596126974
8	79.02878882547392	5.165344982048492	14.542822717163107	1.2630434753144733
9	76.05001755168259	4.9727508433617365	16.478929171167533	2.498302433788141
10	33.048801809653014	32.760927108336574	22.79536635579632	11.394904726214081
11	25.197168418898354	25.045776685673083	34.7168215597008	15.04023333572777
12	24.018562767934228	22.247130401547256	36.815704622123796	16.91860220839472
13	21.57759131279809	23.79208507212454	37.372072630082364	17.258250984995005
14	19.223632832353648	26.33714681474233	36.92331879954623	17.515901553357793
15	18.74031777355212	24.40795260639098	40.2376728230831	16.61405679697379
16	21.392722625135367	23.061284512097107	38.91960245100099	16.62639041176654
17	21.37591638080239	22.854865882104196	36.443392096457	19.325825640636417
18	21.77506468371055	23.19803854864527	37.30118822858119	17.725708539063
19	23.787205839898835	24.427469535293792	34.254514306315485	17.53081031849188
20	25.02693742791273	24.1240083977008	34.24218069152274	16.60687348286373
21	24.020595781361603	24.00148545514427	34.992227111996	16.98569165149813
22	22.388763670321122	23.063859662438453	35.607959112033946	18.939417555206482
23	20.83269519300752	25.36983902599682	35.42946053311034	18.36800524788533
24	20.81100971644884	24.0616626525946	37.21173563777664	17.915591993179916
25	21.107016471474786	25.14173491944523	35.14592292710563	18.605325681974357
26	19.593641276136083	26.745918047873403	34.60378601313869	19.05665466285183
27	19.794503002760834	25.222648853854796	35.9465236148063	19.03632452857807
28	19.489957591339905	25.824149759901115	36.80621722612937	17.879675422629607
29	20.43354689009936	24.76860918840749	36.244698917488115	18.553145004005035
30	20.867663023958386	24.51936174221119	36.06470946205109	18.548265771779334
31	22.26651179622158	25.303833856721347	34.07696446699131	18.35268988006576
32	22.595724437228	25.477317669190764	33.640950853933404	18.286007039647828
33	22.391474354890956	26.110669118932638	32.79182891243269	18.706027613743714
34	21.217476867695552	27.247123624835833	32.76472206673434	18.77067744073427
35	21.305303047758194	27.05005685660885	32.21472416751489	19.429915928118067
36	22.235067855211494	28.3971315535882	30.13833978702151	19.229460804178792
37	22.261497029767384	28.945638576294254	29.890583217338623	18.902281176599743
38	22.1021087770611	28.903216362776334	29.60352172139313	19.39115313876943
39	22.203894982658394	28.246688559962372	29.279459381069394	20.269957076309836
40	22.649260457482235	27.246852556378855	30.08426162985331	20.0196253562856
41	20.609741387138612	27.710650686277567	30.383521206363064	21.296086720220757
42	21.663791082118834	28.347526025960224	29.08469669472677	20.903986197194172
43	21.507384582439375	27.862449022188308	29.499024831226002	21.131141564146315
44	21.498981460272883	28.05084159979182	30.014597036408556	20.435579903526737
45	21.367784327092888	28.07686417166223	29.47517080701146	21.080180694233423
46	21.08519546068762	28.926528250076917	29.589561695858478	20.398714593376983
47	21.16800687429607	28.103157811989625	30.340014719017216	20.388820594697087
48	21.84256072949943	26.79281289093154	30.745939733349957	20.61868664621907
49	21.405327308385097	27.3395579686672	30.621925914280023	20.633188808667686
50	20.459840530426757	27.880204006120724	30.851791965802004	20.808163497650515
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	398.0
1	691.0
2	984.0
3	11156.5
4	21329.0
5	15584.0
6	9839.0
7	9904.0
8	9969.0
9	10075.5
10	10182.0
11	10022.5
12	9863.0
13	9619.5
14	9376.0
15	8819.5
16	8263.0
17	7781.5
18	7300.0
19	6988.5
20	6677.0
21	6582.0
22	6487.0
23	6515.5
24	6544.0
25	7449.5
26	8355.0
27	9118.5
28	9882.0
29	11131.5
30	12381.0
31	14629.5
32	16878.0
33	19255.5
34	21633.0
35	23775.0
36	25917.0
37	27870.5
38	29824.0
39	32370.0
40	34916.0
41	37561.0
42	40206.0
43	42540.0
44	44874.0
45	46081.0
46	47288.0
47	47810.0
48	48332.0
49	48061.5
50	47791.0
51	44554.0
52	41317.0
53	39018.0
54	36719.0
55	34553.0
56	32387.0
57	31038.0
58	29689.0
59	28646.0
60	27603.0
61	25423.0
62	23243.0
63	20205.5
64	17168.0
65	14254.0
66	11340.0
67	9476.0
68	7612.0
69	6481.5
70	5351.0
71	4681.0
72	4011.0
73	3307.5
74	2604.0
75	2106.5
76	1609.0
77	1244.0
78	879.0
79	659.5
80	440.0
81	321.5
82	203.0
83	150.5
84	98.0
85	67.0
86	36.0
87	25.5
88	15.0
89	9.5
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	737821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.71096848399145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.580677480091	33.17733060839188
2	8.31919479993369	7.60556902623909
3	4.7318869057799375	6.488973996597556
4	3.3480288736041834	6.121665692992569
5	2.5025997539756877	5.719812924101371
6	1.87909991744851	5.153728626285586
7	1.452375356299098	4.647263889709975
8	1.140642058344526	4.171188254440141
9	0.9021398557610014	3.7113917863359354
>10	3.11412265963745	20.296698583718236
>50	0.02155519314575681	0.641799759847479
>100	0.007381871133761799	0.610077921572459
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.952748453449843E-4	1.6544989297677777
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12258	1.6613785728516808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01097827250783049	0.0
2	1.3553422849173444E-4	0.0	0.0	0.04770804842909052	0.0
3	1.3553422849173444E-4	0.0	0.0	0.10029532908388349	0.0
4	1.3553422849173444E-4	0.0	0.0	0.14840998019844923	0.0
5	1.3553422849173444E-4	0.0	0.0	0.23271227032030803	0.0
6	1.3553422849173444E-4	0.0	0.0	0.46366259567022355	0.0
7	1.3553422849173444E-4	0.0	0.0	0.6096329597558215	0.0
8	1.3553422849173444E-4	0.0	0.0	0.8928994973035466	0.0
9	1.3553422849173444E-4	0.0	0.0	1.0905084024444953	0.0
10	1.3553422849173444E-4	0.0	0.0	1.3092606472301547	0.0
11	1.3553422849173444E-4	0.0	0.0	1.429072905216848	0.0
12	1.3553422849173444E-4	0.0	0.0	1.5375002880102355	0.0
13	1.3553422849173444E-4	0.0	0.0	1.6284437553281894	0.0
14	1.3553422849173444E-4	0.0	0.0	1.678726954098623	0.0
15	1.3553422849173444E-4	0.0	0.0	1.7200648937886018	0.0
16	1.3553422849173444E-4	0.0	0.0	1.7696704214165766	0.0
17	1.3553422849173444E-4	0.0	0.0	1.8199536201870101	0.0
18	1.3553422849173444E-4	0.0	0.0	1.905746786822278	0.0
19	1.3553422849173444E-4	0.0	0.0	1.9549457117647777	0.0
20	1.3553422849173444E-4	0.0	0.0	2.0122766904167815	0.0
21	1.3553422849173444E-4	0.0	0.0	2.068523395240851	0.0
22	1.3553422849173444E-4	0.0	0.0	2.1244990316079373	0.0
23	1.3553422849173444E-4	0.0	0.0	2.198229651907441	0.0
24	1.3553422849173444E-4	0.0	0.0	2.2536631513605605	0.0
25	1.3553422849173444E-4	0.0	0.0	2.3088255823566963	0.0
26	1.3553422849173444E-4	0.0	0.0	2.3648012187237826	0.0
27	1.3553422849173444E-4	0.0	0.0	2.424978416174113	0.0
28	1.3553422849173444E-4	0.0	0.0	2.4893571747076866	0.0
29	1.3553422849173444E-4	0.0	0.0	2.5609192473513223	0.0
30	1.3553422849173444E-4	0.0	0.0	2.651862714669276	0.0
31	1.3553422849173444E-4	0.0	0.0	2.7270842114821887	0.0
32	1.3553422849173444E-4	0.0	0.0	2.812741843888965	0.0
33	9.487395994421411E-4	0.0	0.0	2.8975862709247906	0.0
34	9.487395994421411E-4	0.0	0.0	2.984328177159501	0.0
35	9.487395994421411E-4	0.0	0.0	3.0752716444774544	0.0
36	9.487395994421411E-4	0.0	0.0	3.164182098368032	0.0
37	9.487395994421411E-4	0.0	0.0	3.2690855912206347	0.0
38	9.487395994421411E-4	0.0	0.0	3.372091604874353	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTATG	20	7.8546465E-4	44.0	1
ACGAACG	20	7.8546465E-4	44.0	1
CGTTTTT	9535	0.0	42.8925	1
GTTTTTT	10520	0.0	38.625477	2
GCCGATA	35	7.2866624E-6	37.714287	9
AGTACGG	35	7.2866624E-6	37.714287	2
GGCACCG	305	0.0	37.508198	8
GACCGAT	500	0.0	37.4	9
GTTACGG	65	1.0913936E-11	37.23077	2
AACGTAG	30	1.3006433E-4	36.666668	1
GCGCGAC	180	0.0	36.666668	9
TGCGACG	30	1.3006433E-4	36.666668	1
TTACGGG	150	0.0	36.666668	3
AATCGTT	30	1.3006433E-4	36.666668	22
ATAGGGA	910	0.0	36.021976	4
TACGAAT	55	3.421519E-9	36.000004	12
CGAATAT	55	3.421519E-9	36.000004	14
CGACCCG	190	0.0	35.894737	32
TAGGGAT	1065	0.0	35.737087	5
TGGGCGA	425	0.0	35.717648	6
>>END_MODULE