FastQCFastQC Report
Thu 26 May 2016
SRR1547203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547203_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1822854
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243031.3332389758038767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA207.857699E-444.023
CGTTTTT189500.042.258571
CGTTAGG2150.037.8604662
CTATGCG700.037.7142871
TACGGGA5600.037.321434
GTTTTTT217400.037.0680772
GGGCGAT40250.036.7850957
GACCGAT12550.036.637459
ACGGGAT6400.036.43755
ACCCGCT8500.035.9764734
TCACGAC1600.035.7525
GTATACG800.035.751
CGTAAGG2650.035.6981122
GCCAGTA9800.035.6938825
TAGGGAC14950.035.612045
TAGGGCG9600.035.5208325
AGGGCGA22700.035.4713676
CTATACG505.948823E-835.21
GCGCGAC8650.035.0982679
TATACGG1950.034.9743582
Produced by FastQC (version 0.11.5)