##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547203_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1822854
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.539846306945044	31.0	30.0	31.0	27.0	34.0
2	30.8798302003342	31.0	31.0	33.0	28.0	34.0
3	30.975406697409667	31.0	31.0	34.0	28.0	34.0
4	34.833644384026364	35.0	35.0	37.0	32.0	37.0
5	34.65743060058568	35.0	35.0	37.0	31.0	37.0
6	34.68803700131772	35.0	35.0	37.0	31.0	37.0
7	35.348171603430664	35.0	35.0	37.0	33.0	37.0
8	34.30747059281764	35.0	35.0	37.0	32.0	37.0
9	36.39445726317083	39.0	37.0	39.0	32.0	39.0
10	35.82401827025094	37.0	35.0	39.0	30.0	39.0
11	35.59654366175239	37.0	35.0	39.0	30.0	39.0
12	35.34249753408666	37.0	34.0	39.0	30.0	39.0
13	35.26928486867297	37.0	34.0	39.0	30.0	39.0
14	36.1659035775767	38.0	34.0	40.0	29.0	41.0
15	36.28273026803024	38.0	34.0	40.0	30.0	41.0
16	36.30830719300613	38.0	34.0	40.0	30.0	41.0
17	36.1793873782541	38.0	34.0	40.0	30.0	41.0
18	36.02424988507033	38.0	34.0	40.0	29.0	41.0
19	35.913435195577925	37.0	34.0	40.0	29.0	41.0
20	35.683330096650636	37.0	34.0	40.0	29.0	41.0
21	35.51358364410973	37.0	34.0	40.0	29.0	41.0
22	35.47759118393464	37.0	34.0	40.0	28.0	41.0
23	35.33954063243683	37.0	33.0	40.0	28.0	41.0
24	35.18574444250609	37.0	33.0	40.0	27.0	41.0
25	34.96775221712765	36.0	33.0	40.0	27.0	41.0
26	34.778104006135436	36.0	33.0	40.0	26.0	41.0
27	34.570534447629925	36.0	33.0	40.0	25.0	41.0
28	34.535612835696114	36.0	33.0	40.0	25.0	41.0
29	34.3051478615402	36.0	33.0	39.0	24.0	41.0
30	34.24459611137261	36.0	33.0	39.0	24.0	41.0
31	34.111070332566406	36.0	33.0	40.0	24.0	41.0
32	33.71176298266345	36.0	32.0	40.0	22.0	41.0
33	33.366238327370155	36.0	31.0	40.0	21.0	41.0
34	33.03648674002416	36.0	31.0	40.0	18.0	41.0
35	32.74624517377695	36.0	31.0	40.0	17.0	41.0
36	32.63020132166372	36.0	31.0	40.0	16.0	41.0
37	32.61375458484333	36.0	31.0	40.0	15.0	41.0
38	32.58580445828355	36.0	31.0	40.0	15.0	41.0
39	32.45761152566251	35.0	30.0	40.0	15.0	41.0
40	32.36053024542832	35.0	30.0	40.0	15.0	41.0
41	32.33875121101306	35.0	30.0	40.0	15.0	41.0
42	32.17062145404953	35.0	30.0	39.0	14.0	41.0
43	32.18824491703669	35.0	30.0	39.0	15.0	41.0
44	31.87380722756732	35.0	30.0	39.0	12.0	41.0
45	31.792833106765546	35.0	29.0	39.0	12.0	41.0
46	31.73153911393891	35.0	29.0	39.0	12.0	41.0
47	31.655869312627342	35.0	29.0	39.0	12.0	40.0
48	31.639501572808353	35.0	29.0	39.0	12.0	40.0
49	31.630361510027683	35.0	29.0	39.0	12.0	40.0
50	31.10295777939429	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	5.0
13	22.0
14	40.0
15	133.0
16	437.0
17	1201.0
18	2482.0
19	4811.0
20	7723.0
21	11558.0
22	16301.0
23	22257.0
24	30371.0
25	41107.0
26	53827.0
27	61296.0
28	64309.0
29	66968.0
30	73642.0
31	84376.0
32	98315.0
33	117281.0
34	150542.0
35	164833.0
36	162190.0
37	185260.0
38	210338.0
39	191223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.037563074168304	22.15300841427783	24.82502712778972	20.984401383764144
2	37.54754906317236	23.664539233531595	26.23567219316522	12.552239510130816
3	27.589044432521746	24.684642873208716	34.77958190837006	12.946730785899474
4	24.46537133527973	26.948729848907266	34.38421288814134	14.201685927671662
5	21.487074664235315	30.34966047747104	34.191054247899174	13.97221061039447
6	19.897314869978615	38.58893800600597	31.939968862015277	9.573778262000138
7	82.20779064039138	3.2576937044875782	13.077240415304791	1.4572752398162443
8	80.05440918471804	5.5921648140772655	12.651644070232724	1.7017819309719813
9	77.08686488330936	5.070674886743535	14.902126006800327	2.9403342231467797
10	36.588503522498236	29.706438365332605	20.988351233834415	12.716706878334744
11	29.270857677027344	24.595551810512525	30.334354808448726	15.799235704011402
12	26.790735846096286	21.928964140847267	33.67378846577949	17.606511547276963
13	23.50572234528931	24.091945926552537	34.48416603853079	17.918165689627365
14	20.274086679459792	26.53125264009076	35.23578959148676	17.95887108896269
15	20.0345173008919	22.89272755799422	38.93849973722525	18.134255403888627
16	22.80155185220539	21.622137593027198	37.312203829818515	18.2641067249489
17	22.182961443977412	21.494700069232096	34.79154117663839	21.530797310152103
18	23.06108991723967	22.1410491460095	35.487702251524254	19.310158685226572
19	26.032309773574845	23.89417912789505	31.694749003485747	18.378762095044365
20	26.88909808465187	23.674578435793542	31.321268735729795	18.115054743824793
21	25.154620172542618	24.268921153312334	32.85013500806976	17.726323666075285
22	24.032039867153376	22.374748608500735	32.86736074309846	20.72585078124743
23	22.485015256295895	24.928052383789375	32.59405306184697	19.992879298067756
24	22.492750379350184	23.619993702183496	35.04987234304009	18.837383575426227
25	22.77988253584763	24.236280031203815	32.965997276797815	20.01784015615074
26	20.603789442270198	25.59579648178077	32.605847753029046	21.194566322919993
27	20.859706811406728	24.496311827496882	33.62929779346015	21.014683567636244
28	20.7096125087363	24.92969815465199	34.70305356325849	19.657635773353213
29	21.759778896170513	24.145598056673766	34.23088190277444	19.863741144381283
30	21.9662134213711	24.306499588008695	33.87413363878841	19.8531533518318
31	24.445512366870854	24.008121330616714	31.75460020385615	19.791766098656282
32	24.9233893663453	24.1640855493638	31.036714953583775	19.875810130707123
33	24.558412248046196	24.972159042907442	30.05369601734423	20.415732691702132
34	21.906087925856927	26.116518382711945	31.26580625765969	20.711587433771435
35	22.340461715529603	26.27933998005326	30.52208240484427	20.85811589957287
36	23.847932966655584	27.654491253825046	27.925604573926382	20.571971205592988
37	22.860251012971965	28.64096630887608	27.973441646999703	20.525341031152248
38	23.120611963437554	28.651170088224294	27.41256293702074	20.815655011317418
39	22.802539314722956	27.705455291537334	27.394898329762015	22.0971070639777
40	23.35875500725785	25.99895548409253	28.883827229169206	21.75846227948042
41	21.21810084625538	26.39021007716471	29.08000311599283	23.31168596058708
42	22.45138667166981	27.111002855961036	27.463965846963056	22.973644625406095
43	21.779583005550638	26.469097360512688	28.788811391367602	22.962508242569072
44	21.92473999563322	26.61705216106172	29.40032498488634	22.057882858418722
45	21.524543380874167	27.27371473524484	28.983506084414877	22.218235799466115
46	22.570101609893058	27.489255859218563	28.472768526716898	21.46787400417148
47	21.575068546356427	27.099263023807723	29.152417033947863	22.173251395887984
48	22.030179048898045	26.257670663695503	29.895592296475748	21.816557990930704
49	22.44233493192543	25.79866517011236	30.151290229497263	21.607709668464945
50	21.014903003751257	26.434261877254023	30.337207477943927	22.213627641050792
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1148.0
1	1980.5
2	2813.0
3	22839.0
4	42865.0
5	31418.0
6	19971.0
7	19894.5
8	19818.0
9	19970.5
10	20123.0
11	20223.5
12	20324.0
13	19656.0
14	18988.0
15	18257.0
16	17526.0
17	16525.0
18	15524.0
19	14689.5
20	13855.0
21	13446.5
22	13038.0
23	13593.5
24	14149.0
25	15332.5
26	16516.0
27	18203.0
28	19890.0
29	22294.0
30	24698.0
31	29355.0
32	34012.0
33	38779.5
34	43547.0
35	48777.5
36	54008.0
37	57897.0
38	61786.0
39	68422.0
40	75058.0
41	81205.5
42	87353.0
43	94946.5
44	102540.0
45	105517.0
46	108494.0
47	111606.5
48	114719.0
49	119437.5
50	124156.0
51	120186.0
52	116216.0
53	109161.0
54	102106.0
55	97505.0
56	92904.0
57	91195.0
58	89486.0
59	85300.0
60	81114.0
61	75867.5
62	70621.0
63	62791.5
64	54962.0
65	47232.5
66	39503.0
67	33833.0
68	28163.0
69	25177.0
70	22191.0
71	18963.5
72	15736.0
73	12963.0
74	10190.0
75	8089.5
76	5989.0
77	4639.5
78	3290.0
79	2583.5
80	1877.0
81	1345.5
82	814.0
83	601.5
84	389.0
85	311.0
86	233.0
87	171.5
88	110.0
89	68.0
90	26.0
91	18.5
92	11.0
93	6.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1822854.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.896238486422916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4163409684771	24.37393835790356
2	5.7035533885006675	3.6384379819932566
3	2.6674169313160814	2.5524169975194035
4	1.7478399028292295	2.2299807350690943
5	1.3394057237806842	2.1361002197894305
6	1.1530488687865044	2.20667530231887
7	0.9620247146992021	2.1479478810915107
8	0.8370580539070114	2.135920265151927
9	0.7766785397122549	2.22958115369539
>10	8.240887592291314	50.707669887583506
>50	0.1292829697047644	2.601614820488861
>100	0.024885146215620352	1.3959157096347274
>500	0.0014019553595713574	0.28535101030391974
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.7524441994641967E-4	1.3584496774566428
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24303	1.3332389758038767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010423215463224153	0.0
2	0.0	0.0	0.0	0.0392242055589751	0.0
3	0.0	0.0	0.0	0.0803684771243336	0.0
4	0.0	0.0	0.0	0.12452999527115172	0.0
5	0.0	0.0	0.0	0.2028138293028405	0.0
6	0.0	0.0	0.0	0.4292718999985737	0.0
7	0.0	0.0	0.0	0.5770072644325875	0.0
8	0.0	0.0	0.0	0.9350172860799604	0.0
9	0.0	0.0	0.0	1.152588194117576	0.0
10	0.0	0.0	0.0	1.4082312681103368	0.0
11	0.0	0.0	0.0	1.543403914959728	0.0
12	0.0	0.0	0.0	1.6639292011318514	0.0
13	0.0	0.0	0.0	1.7427616254510783	0.0
14	0.0	0.0	0.0	1.7896660950355872	0.0
15	0.0	0.0	0.0	1.8226912303453815	0.0
16	0.0	0.0	0.0	1.8700345721599207	0.0
17	0.0	0.0	0.0	1.9205048786134271	0.0
18	0.0	0.0	0.0	2.0119548795460305	0.0
19	0.0	0.0	0.0	2.054360908772727	0.0
20	0.0	0.0	0.0	2.1037888936799107	0.0
21	0.0	0.0	0.0	2.15875764049123	0.0
22	0.0	0.0	0.0	2.2177859554303305	0.0
23	0.0	0.0	0.0	2.282135596158551	0.0
24	0.0	0.0	0.0	2.336281457538563	0.0
25	0.0	0.0	0.0	2.390756473091098	0.0
26	0.0	0.0	0.0	2.4435857177810183	0.0
27	0.0	0.0	0.0	2.50003565836869	0.0
28	0.0	0.0	0.0	2.5618069247454813	0.0
29	0.0	0.0	0.0	2.6295578252564384	0.0
30	0.0	0.0	0.0	2.717771143492567	0.0
31	0.0	0.0	0.0	2.7865095065210927	0.0
32	0.0	0.0	0.0	2.8725833226358226	0.0
33	0.0	0.0	0.0	2.955365597025324	0.0
34	0.0	0.0	0.0	3.0363923824947032	0.0
35	0.0	0.0	0.0	3.124331405587063	0.0
36	0.0	0.0	0.0	3.2110086710180847	0.0
37	0.0	0.0	0.0	3.30075804205932	0.0
38	0.0	0.0	0.0	3.4108052537394657	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGTA	20	7.857699E-4	44.0	23
CGTTTTT	18950	0.0	42.25857	1
CGTTAGG	215	0.0	37.860466	2
CTATGCG	70	0.0	37.714287	1
TACGGGA	560	0.0	37.32143	4
GTTTTTT	21740	0.0	37.068077	2
GGGCGAT	4025	0.0	36.785095	7
GACCGAT	1255	0.0	36.63745	9
ACGGGAT	640	0.0	36.4375	5
ACCCGCT	850	0.0	35.97647	34
TCACGAC	160	0.0	35.75	25
GTATACG	80	0.0	35.75	1
CGTAAGG	265	0.0	35.698112	2
GCCAGTA	980	0.0	35.69388	25
TAGGGAC	1495	0.0	35.61204	5
TAGGGCG	960	0.0	35.520832	5
AGGGCGA	2270	0.0	35.471367	6
CTATACG	50	5.948823E-8	35.2	1
GCGCGAC	865	0.0	35.098267	9
TATACGG	195	0.0	34.974358	2
>>END_MODULE