FastQCFastQC Report
Thu 26 May 2016
SRR1547202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547202_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3078332
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242780.7886738662366503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT206700.042.605711
ACGGGTA2500.039.65
TAGTACG1350.039.111111
TAACGCG404.1275416E-738.51
AGGGCGA32800.038.56
TCGATAG1150.038.260871
ACGGGAT13750.038.085
TACGGGA11100.037.8558584
AACGGGA12000.037.5833324
AAGGGCG16050.037.146425
GGGCGAT66650.037.035267
CGTTAGG5100.036.6666682
TGGGCGA19850.036.4634786
GTTTTTT241650.036.3525732
AGGGATT64150.036.3180056
AGTACGG3350.036.1194042
GGCGATT19150.035.9582258
TAGGGAC33550.035.868855
AAGGGAT59700.035.4137345
CGGGATC7900.035.367096
Produced by FastQC (version 0.11.5)