##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547202_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3078332
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.670184047724547	31.0	30.0	31.0	28.0	34.0
2	31.004818843451584	31.0	31.0	33.0	28.0	34.0
3	31.138461998251	31.0	31.0	34.0	28.0	34.0
4	34.97596230685969	35.0	35.0	37.0	32.0	37.0
5	34.79140196703929	35.0	35.0	37.0	32.0	37.0
6	34.83350496307741	35.0	35.0	37.0	32.0	37.0
7	35.490507846457106	35.0	35.0	37.0	35.0	37.0
8	34.46771758211915	37.0	35.0	37.0	33.0	37.0
9	36.58423717779629	39.0	37.0	39.0	32.0	39.0
10	35.9867113098912	38.0	35.0	39.0	30.0	39.0
11	35.74454867116347	37.0	35.0	39.0	30.0	39.0
12	35.55000987547802	37.0	35.0	39.0	30.0	39.0
13	35.45775731792412	37.0	35.0	39.0	30.0	39.0
14	36.41062529967528	38.0	35.0	40.0	30.0	41.0
15	36.54896645326105	38.0	35.0	40.0	30.0	41.0
16	36.592072589961056	38.0	35.0	40.0	31.0	41.0
17	36.49734401617499	38.0	35.0	40.0	30.0	41.0
18	36.38035241163072	38.0	35.0	40.0	30.0	41.0
19	36.32250290092167	38.0	35.0	40.0	30.0	41.0
20	36.16565464673726	38.0	34.0	40.0	30.0	41.0
21	36.014603363120024	38.0	34.0	40.0	30.0	41.0
22	35.98144222260627	38.0	34.0	40.0	30.0	41.0
23	35.85498445261915	38.0	34.0	40.0	29.0	41.0
24	35.75829800034564	38.0	34.0	40.0	29.0	41.0
25	35.577667710955154	37.0	34.0	40.0	29.0	41.0
26	35.413791624815	37.0	34.0	40.0	27.0	41.0
27	35.235164693087036	37.0	34.0	40.0	27.0	41.0
28	35.23511271688694	37.0	34.0	40.0	27.0	41.0
29	35.08983663880309	37.0	33.0	40.0	27.0	41.0
30	35.079965708701984	37.0	33.0	40.0	27.0	41.0
31	35.00428576254933	37.0	33.0	40.0	26.0	41.0
32	34.70281957891481	37.0	33.0	40.0	25.0	41.0
33	34.46030415172892	37.0	33.0	40.0	24.0	41.0
34	34.27835561596345	37.0	33.0	40.0	23.0	41.0
35	34.05180727744766	37.0	33.0	40.0	23.0	41.0
36	33.98868867945368	37.0	33.0	40.0	22.0	41.0
37	33.994367729016886	37.0	33.0	40.0	22.0	41.0
38	33.994060419733806	37.0	33.0	40.0	22.0	41.0
39	33.90050748262371	37.0	33.0	40.0	21.0	41.0
40	33.84236690519411	37.0	33.0	40.0	21.0	41.0
41	33.85645960214818	37.0	33.0	40.0	21.0	41.0
42	33.705811783784206	37.0	32.0	40.0	21.0	41.0
43	33.75555138302171	37.0	32.0	40.0	21.0	41.0
44	33.39255317490121	36.0	32.0	40.0	19.0	41.0
45	33.3556490982779	36.0	32.0	40.0	19.0	41.0
46	33.32098974379632	36.0	32.0	40.0	20.0	41.0
47	33.28044018643863	36.0	31.0	40.0	20.0	41.0
48	33.2856504756472	36.0	32.0	40.0	20.0	41.0
49	33.282629034165254	36.0	32.0	40.0	20.0	41.0
50	32.74121082456343	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	6.0
13	16.0
14	27.0
15	115.0
16	403.0
17	1098.0
18	2305.0
19	4607.0
20	7795.0
21	12008.0
22	17858.0
23	25361.0
24	35962.0
25	49871.0
26	65925.0
27	77829.0
28	84986.0
29	93909.0
30	109906.0
31	131139.0
32	159125.0
33	193588.0
34	255656.0
35	292569.0
36	287334.0
37	338589.0
38	410505.0
39	419837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.823695429862664	23.695949624666866	27.319437929372143	17.160917016098328
2	33.1160186750487	25.261245375742448	28.466162844033715	13.15657310517514
3	27.560250161451073	25.531359190626613	33.528157456700576	13.380233191221741
4	24.181992065833054	28.129487007899083	33.16114051375875	14.52738041250911
5	21.24874769842889	32.62861185862994	32.573971878276936	13.54866856466424
6	19.89518999250243	41.03410548309929	29.31516158750908	9.7555429368892
7	87.18205183846317	2.841961165982097	8.777058484919756	1.1989285106349803
8	85.34115228636807	5.093375243476013	8.347150339859379	1.2183221302965372
9	82.05148112679204	5.2604787267910025	10.205786770237909	2.4822533761790475
10	35.34202938474473	35.85851038809329	16.99517790803591	11.804282319126072
11	27.444538145982953	25.716426948100462	30.814674960335665	16.02435994558092
12	26.541646580031003	23.173653783932338	32.46209310756605	17.822606528470615
13	22.073220172483023	27.761755392205913	32.89492491388194	17.270099521429138
14	19.338654829953363	30.412314201327213	32.39387434493745	17.85515662378197
15	18.64301836189209	25.789518479488244	38.61792685129479	16.949536307324877
16	21.231595552396556	24.30062774255668	36.63711386556096	17.8306628394858
17	21.134302602838158	23.93975048825143	32.3170795092927	22.608867399617715
18	22.29665286265419	24.07073051249833	34.24360985104921	19.38900677379828
19	24.5836056669651	25.67750976827711	30.611577958452823	19.127306606304973
20	26.075972312278207	25.4194154496656	30.29968827273991	18.20492396531628
21	23.616913315392882	26.30362806870734	32.15842215849363	17.921036457406153
22	23.526214846221915	24.053253515215385	31.05961280329737	21.360918835265334
23	20.56519569689039	27.85229143575157	31.557090008485115	20.025422858872922
24	20.321589744056194	25.61796453403986	35.35700502739795	18.703440694505986
25	20.39247228693981	26.95056933430182	33.035390594646714	19.621567784111654
26	20.123820302683402	29.056092715145738	30.845016067142854	19.97507091502801
27	19.97562316215405	27.996655331523694	32.14796194822391	19.879759558098346
28	19.0647727405621	27.155128166812414	34.50985793605108	19.270241156574404
29	19.690046427740736	25.988100048987565	34.381736602809575	19.94011692046212
30	20.423073274747495	27.12475457488016	32.98750752030645	19.464664630065894
31	22.970329386174072	26.681397588044437	30.55037598283746	19.797897042944037
32	23.536480145741265	26.34192803115453	31.108762797515016	19.012829025589184
33	23.022240616021925	27.75922155245113	29.56035931147128	19.658178520055667
34	20.235731558519355	27.711208537610627	31.209174319079292	20.843885584790726
35	20.67619736922463	27.73752148891023	31.126662101423758	20.459619040441382
36	23.290795144903147	28.423574845078438	28.6761791775546	19.609450832463814
37	20.85905613819432	29.700077834359647	29.742243526689126	19.698622500756905
38	21.29354468588833	30.02359719484448	28.61341791593629	20.069440203330895
39	21.020539694873715	29.453548220269937	28.83022364059497	20.69568844426137
40	22.518331356072053	27.368328042589297	29.245643419878036	20.86769718146061
41	20.100073676263637	26.94481946716599	29.9026550742415	23.052451782328873
42	21.183127745805198	27.561094774702667	28.739200320173392	22.51657715931875
43	20.85960838532036	27.92229688025853	29.353169183830722	21.86492555059038
44	20.735807573712	28.945058557686437	29.544539055566453	20.77459481303511
45	20.250642230922463	30.118713641023774	28.648534336127483	20.982109791926277
46	21.058807172195852	29.756764377591498	28.76064050271381	20.423787947498838
47	21.069689689091366	28.557413560330723	29.17014798923573	21.202748761342182
48	21.75584050063476	27.238452512594485	30.13222745304925	20.873479533721508
49	21.084080599493493	27.147364221922782	30.297771650361298	21.470783528222427
50	20.13723665933369	29.79990462367282	29.242167511496486	20.820691205497003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1570.0
1	2381.5
2	3193.0
3	24059.5
4	44926.0
5	33405.5
6	21885.0
7	21971.0
8	22057.0
9	22527.5
10	22998.0
11	22755.0
12	22512.0
13	22382.0
14	22252.0
15	21506.5
16	20761.0
17	19864.5
18	18968.0
19	18742.5
20	18517.0
21	19119.5
22	19722.0
23	21391.0
24	23060.0
25	27442.5
26	31825.0
27	37484.0
28	43143.0
29	50790.5
30	58438.0
31	67026.0
32	75614.0
33	88896.0
34	102178.0
35	111779.5
36	121381.0
37	131161.5
38	140942.0
39	155990.5
40	171039.0
41	190897.5
42	210756.0
43	226664.5
44	242573.0
45	247502.0
46	252431.0
47	244172.5
48	235914.0
49	223842.5
50	211771.0
51	201976.0
52	192181.0
53	176272.0
54	160363.0
55	145665.0
56	130967.0
57	122606.0
58	114245.0
59	104505.0
60	94765.0
61	84517.0
62	74269.0
63	63565.5
64	52862.0
65	43656.0
66	34450.0
67	28737.5
68	23025.0
69	19444.5
70	15864.0
71	12720.0
72	9576.0
73	7992.0
74	6408.0
75	5341.5
76	4275.0
77	3384.0
78	2493.0
79	1809.5
80	1126.0
81	880.5
82	635.0
83	405.5
84	176.0
85	151.5
86	127.0
87	85.0
88	43.0
89	42.5
90	42.0
91	24.5
92	7.0
93	6.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3078332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.590666313933756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10421304551954	18.897117602971107
2	5.823996348809119	2.7478390895664893
3	2.1147553620134194	1.4966546424258227
4	1.25434897386176	1.1836371233439196
5	0.8578573493585	1.0118713236836032
6	0.6474131302723266	0.9163744274108269
7	0.5564202833835176	0.9188427664923514
8	0.4731405650605631	0.8929360951943848
9	0.40053896132113487	0.850408288403086
>10	5.725467443150275	32.909313047621666
>50	1.774432303767039	28.842589233979915
>100	0.2658612798029534	8.234378934059698
>500	0.0011308754035420383	0.15623364823423058
>1k	2.8271885088550946E-4	0.13219000674503517
>5k	0.0	0.0
>10k+	1.4135942544275473E-4	0.8096137698678013
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24278	0.7886738662366503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01588522615494365	0.0
2	0.0	0.0	0.0	0.06298865749373361	0.0
3	0.0	0.0	0.0	0.11022202933276852	0.0
4	0.0	0.0	0.0	0.16765573044103105	0.0
5	0.0	9.745537518370338E-5	0.0	0.2739795447664514	0.0
6	0.0	9.745537518370338E-5	0.0	0.4974447200626833	0.0
7	0.0	9.745537518370338E-5	0.0	0.6206932845450068	0.0
8	0.0	9.745537518370338E-5	0.0	0.9239744121166917	0.0
9	0.0	9.745537518370338E-5	0.0	1.0899734011795998	0.0
10	0.0	9.745537518370338E-5	0.0	1.3140882789770565	0.0
11	0.0	9.745537518370338E-5	0.0	1.4615057765049384	0.0
12	0.0	9.745537518370338E-5	0.0	1.6017115762692262	0.0
13	0.0	9.745537518370338E-5	0.0	1.6799357574166789	0.0
14	0.0	9.745537518370338E-5	0.0	1.7197950058668137	0.0
15	0.0	9.745537518370338E-5	0.0	1.758354849314499	0.0
16	0.0	9.745537518370338E-5	0.0	1.8307317079509293	0.0
17	0.0	9.745537518370338E-5	0.0	1.9128865892307911	0.0
18	0.0	9.745537518370338E-5	0.0	2.043964068852872	0.0
19	0.0	9.745537518370338E-5	0.0	2.1054908957188503	0.0
20	0.0	9.745537518370338E-5	0.0	2.1749765782248307	0.0
21	0.0	9.745537518370338E-5	0.0	2.2535256106228956	0.0
22	0.0	9.745537518370338E-5	0.0	2.3406832011621876	0.0
23	0.0	9.745537518370338E-5	0.0	2.444862997233567	0.0
24	0.0	9.745537518370338E-5	0.0	2.5236719106321215	0.0
25	0.0	9.745537518370338E-5	0.0	2.5973481742710014	0.0
26	0.0	9.745537518370338E-5	0.0	2.6684581130300433	0.0
27	0.0	9.745537518370338E-5	0.0	2.743206385795944	0.0
28	0.0	9.745537518370338E-5	0.0	2.824809019949765	0.0
29	0.0	9.745537518370338E-5	0.0	2.911999095614118	0.0
30	0.0	9.745537518370338E-5	0.0	3.0288156053343176	0.0
31	0.0	9.745537518370338E-5	0.0	3.127537900395409	0.0
32	0.0	9.745537518370338E-5	0.0	3.2283717285854805	0.0
33	3.248512506123446E-5	9.745537518370338E-5	0.0	3.3249500053925307	0.0
34	3.248512506123446E-5	9.745537518370338E-5	0.0	3.4293896824643997	0.0
35	3.248512506123446E-5	9.745537518370338E-5	0.0	3.5464335880600273	0.0
36	3.248512506123446E-5	9.745537518370338E-5	0.0	3.6559084595163873	0.0
37	3.248512506123446E-5	9.745537518370338E-5	0.0	3.7726924841115252	0.0
38	3.248512506123446E-5	9.745537518370338E-5	0.0	3.8911982203349087	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	20670	0.0	42.60571	1
ACGGGTA	250	0.0	39.6	5
TAGTACG	135	0.0	39.11111	1
TAACGCG	40	4.1275416E-7	38.5	1
AGGGCGA	3280	0.0	38.5	6
TCGATAG	115	0.0	38.26087	1
ACGGGAT	1375	0.0	38.08	5
TACGGGA	1110	0.0	37.855858	4
AACGGGA	1200	0.0	37.583332	4
AAGGGCG	1605	0.0	37.14642	5
GGGCGAT	6665	0.0	37.03526	7
CGTTAGG	510	0.0	36.666668	2
TGGGCGA	1985	0.0	36.463478	6
GTTTTTT	24165	0.0	36.352573	2
AGGGATT	6415	0.0	36.318005	6
AGTACGG	335	0.0	36.119404	2
GGCGATT	1915	0.0	35.958225	8
TAGGGAC	3355	0.0	35.86885	5
AAGGGAT	5970	0.0	35.413734	5
CGGGATC	790	0.0	35.36709	6
>>END_MODULE