FastQCFastQC Report
Thu 26 May 2016
SRR1547201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547201_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1719324
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136960.7965921490074006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG302.5284517E-644.0000041
CGTTTTT90950.041.9681171
CGGTCTA2200.038.031
GTTGATC10400.037.86538316
GGCGATA7950.037.081768
TTATACG301.3013635E-436.6666681
CGAATAT601.9826984E-1036.66666814
CGTAAGG2700.036.6666682
CGGTAGT3850.036.5714312
GGTACCT10950.036.3652958
CATATGC10350.036.34782433
GGGCGAT38000.036.37
TAGGGAC15400.036.2857135
ACGGGTA850.036.2352945
TAACGCC1100.036.012
GCCCTAC10150.035.98029326
GGACTAA11700.035.914538
ATTGGGA12400.035.838714
GTAGCAT10650.035.7370929
GTTTTTT109200.035.659342
Produced by FastQC (version 0.11.5)