##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547201_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1719324
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.560759926575795	31.0	30.0	31.0	27.0	33.0
2	30.894221217176053	31.0	31.0	33.0	28.0	34.0
3	31.0482608280929	31.0	31.0	34.0	28.0	34.0
4	34.89322431374191	35.0	35.0	37.0	32.0	37.0
5	34.696111378658124	35.0	35.0	37.0	32.0	37.0
6	34.718156670877626	35.0	35.0	37.0	31.0	37.0
7	35.344945455306856	35.0	35.0	37.0	33.0	37.0
8	34.312521665491786	35.0	35.0	37.0	32.0	37.0
9	36.39999150829047	39.0	37.0	39.0	32.0	39.0
10	35.8299296700331	37.0	35.0	39.0	30.0	39.0
11	35.61991049970803	37.0	35.0	39.0	30.0	39.0
12	35.30072167898546	37.0	34.0	39.0	30.0	39.0
13	35.215613229385504	37.0	34.0	39.0	30.0	39.0
14	36.11145077949241	38.0	34.0	40.0	29.0	41.0
15	36.250726448301776	38.0	34.0	40.0	30.0	41.0
16	36.29727730200939	38.0	34.0	40.0	30.0	41.0
17	36.197257759444994	38.0	34.0	40.0	30.0	41.0
18	36.10208488917738	38.0	34.0	40.0	30.0	41.0
19	36.0513730977989	38.0	34.0	40.0	30.0	41.0
20	35.89375649964754	38.0	34.0	40.0	29.0	41.0
21	35.717871675146746	38.0	34.0	40.0	29.0	41.0
22	35.675553298854666	37.0	34.0	40.0	29.0	41.0
23	35.533639965474805	37.0	34.0	40.0	28.0	41.0
24	35.41537313502283	37.0	34.0	40.0	27.0	41.0
25	35.179698532679126	37.0	33.0	40.0	27.0	41.0
26	34.98333414760685	37.0	33.0	40.0	27.0	41.0
27	34.76826531822972	37.0	33.0	40.0	26.0	41.0
28	34.74687260807154	37.0	33.0	40.0	25.0	41.0
29	34.54009075659969	36.0	33.0	40.0	25.0	41.0
30	34.53901998692509	36.0	33.0	40.0	25.0	41.0
31	34.47572301672052	37.0	33.0	40.0	25.0	41.0
32	34.219154155935705	36.0	33.0	40.0	24.0	41.0
33	34.01690780795243	36.0	32.0	40.0	23.0	41.0
34	33.83768969664822	36.0	32.0	40.0	23.0	41.0
35	33.60872761620265	36.0	32.0	40.0	22.0	41.0
36	33.54196649380803	36.0	32.0	40.0	21.0	41.0
37	33.54662471994807	36.0	32.0	40.0	21.0	41.0
38	33.52853098078082	36.0	32.0	40.0	21.0	41.0
39	33.404595643404036	36.0	31.0	40.0	21.0	41.0
40	33.30481689315103	36.0	31.0	40.0	20.0	41.0
41	33.28729954330888	36.0	31.0	40.0	20.0	41.0
42	33.10901377518141	35.0	31.0	40.0	20.0	41.0
43	33.14121131328359	35.0	31.0	39.0	20.0	41.0
44	32.82411808361891	35.0	31.0	39.0	18.0	41.0
45	32.739021266497765	35.0	31.0	39.0	19.0	41.0
46	32.669367728246684	35.0	31.0	39.0	19.0	41.0
47	32.5823794700708	35.0	31.0	39.0	19.0	41.0
48	32.556926443183485	35.0	31.0	39.0	19.0	40.0
49	32.5386774104241	35.0	31.0	39.0	19.0	40.0
50	31.98582582456826	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	1.0
12	2.0
13	10.0
14	35.0
15	98.0
16	269.0
17	752.0
18	1607.0
19	3098.0
20	5183.0
21	8206.0
22	11610.0
23	16397.0
24	22586.0
25	30334.0
26	39747.0
27	47312.0
28	53306.0
29	59951.0
30	68611.0
31	80793.0
32	95438.0
33	114962.0
34	149258.0
35	164981.0
36	163750.0
37	186782.0
38	209740.0
39	184500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.21090149384293	23.303926426898013	26.77220814692286	16.712963932336198
2	33.40842098406117	25.444418852991056	27.869209061235694	13.277951101712068
3	28.998955403402732	25.62792120624152	31.86502369535934	13.508099694996407
4	25.556672273521453	28.364229197056517	30.82188115794347	15.257217371478557
5	22.784478085573166	32.15164797327322	30.294522731026845	14.769351210126771
6	20.995519169161835	41.06741952069534	27.66866512652647	10.268396183616352
7	86.70884603483695	3.5155095839992927	8.296051238742669	1.479593142421091
8	84.65385232800799	5.706894104892387	7.926254737326996	1.7129988297726315
9	81.60812040080869	5.236011362605303	10.020391735356455	3.135476501229553
10	38.72894230523159	30.974266630373332	16.459724868611154	13.837066195783922
11	32.89153178807484	25.424759963799726	24.761650509153597	16.92205773897183
12	28.95888151389732	22.251477906432992	29.69614802096638	19.093492558703304
13	24.66672948205225	25.28540286763868	30.782505217166744	19.26536243314233
14	21.301976823449216	27.951450686432576	30.967112655904298	19.77945983421391
15	20.911997971295694	23.400301513850792	35.73346268649772	19.954237828355794
16	23.75648801505708	21.745639565317532	33.69161368072568	20.806258738899707
17	23.031842747498434	22.12049619501618	30.185002943017142	24.662658114468243
18	24.98982158103999	22.54042868010916	31.28752928476541	21.182220454085442
19	27.10640926317553	24.500036060684316	27.41891580644486	20.9746388696953
20	29.283602159918665	23.592062927057377	26.475521774837084	20.64881313818687
21	27.14561071677008	24.588268412469088	28.2740193238738	19.99210154688703
22	26.084728649166767	22.816932701457084	27.959360772024354	23.138977877351795
23	24.19258964569796	25.648452531343715	27.320854010064423	22.838103812893902
24	23.616432970167345	24.241969518252525	31.00235906670296	21.139238444877172
25	24.265350800663516	24.498814650409116	29.267142202400475	21.96869234652689
26	22.929011634805306	25.891455013714694	27.924812309954376	23.254721041525624
27	22.624822313886156	25.124583848070518	29.235269210457133	23.01532462758619
28	22.285677394138624	25.590697274044917	31.113623726534385	21.010001605282074
29	23.221103177760565	24.581405249970338	30.676242523224246	21.521249049044858
30	24.061317122310864	24.26221003138443	30.499545170078473	21.17692767622624
31	26.552121647810417	24.619676105259973	27.387449951259914	21.440752295669693
32	27.248848966221605	24.691041362768157	27.423975934727835	20.6361337362824
33	26.28503993429976	24.771247304173034	26.8844034050592	22.059309356468006
34	23.800284297782152	25.830558987136804	28.404884710502497	21.964272004578543
35	23.656972158825212	25.22712414879336	28.899439547170864	22.21646414521056
36	26.426665363829038	26.22088681365467	26.144635915045683	21.20781190747061
37	24.36760028941607	27.655694912651718	26.19843612954859	21.77826866838362
38	24.3645758449251	27.985708336532266	25.562430350533116	22.087285468009522
39	24.02275545505094	26.79279763441911	25.47041744313463	23.714029467395324
40	24.848137989116655	24.79334901391477	27.443634823919165	22.91487817304941
41	22.53606650055487	25.005699914617608	27.592530552705597	24.865703032121928
42	23.760792032217314	26.138296214093447	25.874529757044044	24.226381996645195
43	23.328412794796094	25.60709906916905	27.200167042395734	23.86432109363913
44	23.237563135278748	25.55527637606408	28.112676842759132	23.09448364589804
45	22.759061119370173	27.004101612028915	27.28851571896862	22.948321549632297
46	23.546230960540303	26.30952630219784	27.486616833127435	22.657625904134417
47	22.817456163003598	27.004683235969484	27.48155670484446	22.696303896182453
48	23.343302367674738	25.727088088109046	28.227314921445874	22.702294622770346
49	23.447878352189583	25.602097103280126	28.169501501753015	22.78052304277728
50	22.488140687851736	26.68316152162129	28.26506231518899	22.563635475337982
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1092.0
1	1701.0
2	2310.0
3	12878.0
4	23446.0
5	16857.5
6	10269.0
7	10448.5
8	10628.0
9	10728.0
10	10828.0
11	10930.5
12	11033.0
13	10724.0
14	10415.0
15	10040.5
16	9666.0
17	9230.5
18	8795.0
19	8503.0
20	8211.0
21	8702.5
22	9194.0
23	9468.5
24	9743.0
25	11220.0
26	12697.0
27	14341.5
28	15986.0
29	18311.5
30	20637.0
31	23852.5
32	27068.0
33	31095.5
34	35123.0
35	39626.0
36	44129.0
37	48320.0
38	52511.0
39	58415.0
40	64319.0
41	71985.0
42	79651.0
43	87353.0
44	95055.0
45	100404.5
46	105754.0
47	111080.0
48	116406.0
49	120779.0
50	125152.0
51	122450.5
52	119749.0
53	112100.0
54	104451.0
55	101190.5
56	97930.0
57	96193.0
58	94456.0
59	92296.5
60	90137.0
61	83868.0
62	77599.0
63	69806.5
64	62014.0
65	54684.5
66	47355.0
67	40768.5
68	34182.0
69	30005.5
70	25829.0
71	22172.5
72	18516.0
73	15221.5
74	11927.0
75	9714.5
76	7502.0
77	5572.5
78	3643.0
79	2850.5
80	2058.0
81	1579.5
82	1101.0
83	755.0
84	409.0
85	318.5
86	228.0
87	143.5
88	59.0
89	54.0
90	49.0
91	27.0
92	5.0
93	5.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1719324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.22135129737496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63836213036453	21.134211297034653
2	5.363756278280003	2.9201738784912092
3	2.2445276219830523	1.832972246839869
4	1.4296626568715791	1.5566939767775876
5	1.123543310863631	1.529218358141733
6	0.9087866730105861	1.4843040768236277
7	0.7616289779769686	1.4512798977440202
8	0.6865133944468379	1.4950257824472575
9	0.5948050721740005	1.457225804079804
>10	8.7211159059494	54.175806240184464
>50	0.4940315521743933	8.233220152244465
>100	0.03043986931941507	1.398523009275269
>500	0.0023917017262944096	0.45017392189211514
>1k	2.1742742966312815E-4	0.07055052785897992
>5k	0.0	0.0
>10k+	2.1742742966312815E-4	0.8106208301649237
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13696	0.7965921490074006	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013551837815327419	0.0
2	0.0	0.0	0.0	0.0597327786967436	0.0
3	0.0	0.0	0.0	0.09672406131712231	0.0
4	0.0	0.0	0.0	0.13691427561064698	0.0
5	0.0	0.0	0.0	0.21543350758786592	0.0
6	0.0	0.0	0.0	0.42074675860977917	0.0
7	0.0	0.0	0.0	0.5245666320018798	0.0
8	0.0	0.0	0.0	0.7953125763381422	0.0
9	0.0	0.0	0.0	0.974220100458087	0.0
10	0.0	0.0	0.0	1.2166409588884934	0.0
11	0.0	0.0	0.0	1.3653622004927517	0.0
12	0.0	0.0	0.0	1.4942500657235054	0.0
13	0.0	0.0	0.0	1.5742815199462115	0.0
14	0.0	0.0	0.0	1.6202298112513989	0.0
15	0.0	0.0	0.0	1.6596639144221799	0.0
16	0.0	0.0	0.0	1.720443616211953	0.0
17	5.816239405719923E-5	0.0	0.0	1.7829681898234422	0.0
18	5.816239405719923E-5	0.0	0.0	1.8868462256096001	0.0
19	5.816239405719923E-5	0.0	0.0	1.9337251152197026	0.0
20	5.816239405719923E-5	0.0	0.0	1.9908987485779295	0.0
21	5.816239405719923E-5	0.0	0.0	2.053248835007247	0.0
22	5.816239405719923E-5	0.0	0.0	2.114842810313821	0.0
23	5.816239405719923E-5	0.0	0.0	2.182020375449886	0.0
24	5.816239405719923E-5	0.0	0.0	2.24175315414663	0.0
25	5.816239405719923E-5	0.0	0.0	2.2963094797722827	0.0
26	5.816239405719923E-5	0.0	0.0	2.3497607199108486	0.0
27	5.816239405719923E-5	0.0	0.0	2.412227131128281	0.0
28	5.816239405719923E-5	0.0	0.0	2.4759149526209137	0.0
29	5.816239405719923E-5	0.0	0.0	2.542161919452064	0.0
30	5.816239405719923E-5	0.0	0.0	2.630626920813064	0.0
31	5.816239405719923E-5	0.0	0.0	2.703678887748906	0.0
32	5.816239405719923E-5	0.0	0.0	2.790166367711961	0.0
33	5.816239405719923E-5	0.0	0.0	2.8722335057266695	0.0
34	5.816239405719923E-5	0.0	0.0	2.9547077804997777	0.0
35	5.816239405719923E-5	0.0	0.0	3.037996328789687	0.0
36	5.816239405719923E-5	0.0	0.0	3.1145380393689615	0.0
37	5.816239405719923E-5	0.0	0.0	3.1952092799262966	0.0
38	5.816239405719923E-5	0.0	0.0	3.293794537853249	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	30	2.5284517E-6	44.000004	1
CGTTTTT	9095	0.0	41.968117	1
CGGTCTA	220	0.0	38.0	31
GTTGATC	1040	0.0	37.865383	16
GGCGATA	795	0.0	37.08176	8
TTATACG	30	1.3013635E-4	36.666668	1
CGAATAT	60	1.9826984E-10	36.666668	14
CGTAAGG	270	0.0	36.666668	2
CGGTAGT	385	0.0	36.57143	12
GGTACCT	1095	0.0	36.365295	8
CATATGC	1035	0.0	36.347824	33
GGGCGAT	3800	0.0	36.3	7
TAGGGAC	1540	0.0	36.285713	5
ACGGGTA	85	0.0	36.235294	5
TAACGCC	110	0.0	36.0	12
GCCCTAC	1015	0.0	35.980293	26
GGACTAA	1170	0.0	35.91453	8
ATTGGGA	1240	0.0	35.83871	4
GTAGCAT	1065	0.0	35.73709	29
GTTTTTT	10920	0.0	35.65934	2
>>END_MODULE